植物

分类

    现刊
    Maintenance and Quantitative Phenotyping of the Oomycete-plant Model Pathosystem Hyaloperonospora arabidopsidisArabidopsis
    卵菌-植物模型病理系统(活体营养型卵菌–拟南芥)的维持和定量表型分析
    作者:Fang-Yu Hwu and Martin Parniske日期:06/20/2020,浏览量:496,Q&A: 0
    [Abstract] The interaction between the host plant Arabidopsis thaliana (Arabidopsis) and the oomycete Hyaloperonospora arabidopsidis (Hpa) is an established model system for the study of an obligate biotrophic downy mildew interaction. The evaluation of the developmental success of Hpa is often based on the quantification ...
    Determination of Ureides Content in Plant Tissues
    植物组织中酰脲含量的测定
    作者:Ignacio Lescano日期:06/05/2020,浏览量:612,Q&A: 0
    [Abstract] The ureides allantoin and allantoate are the main organic nitrogen compounds transported in several legumes, predominantly from N2 fixation. Moreover, recent studies point out a remarkable role for allantoin during several stress responses of plants other than legumes. The goal of this protocol is to determine ureides concentration in ...
    Live Cell Imaging of Male Meiosis in Arabidopsis by a Landmark-based System
    地标系统在拟南芥雄性减数分裂活体细胞成像中的应用
    作者:Maria Ada Prusicki, Emma Mathilde Keizer, Rik Peter van Rosmalen, Christian Fleck and Arp Schnittger日期:05/05/2020,浏览量:1048,Q&A: 0
    [Abstract] Live cell imaging has tremendously promoted our understanding of cellular and subcellular processes such as cell division. Here, we present a step-by-step protocol for a robust and easy-to-use live cell imaging approach to study male meiosis in the plant Arabidopsis thaliana as recently established. Our method relies on the concomitant ...
    Single-run HPLC Quantification of Plant Cell Wall Monosaccharides
    Single-run HPLC定量分析植物细胞壁单糖
    [Abstract] The plant cell wall is a complex network of polysaccharides and proteins that provides strength and structural integrity to plant cells, as well as playing a vital role in growth, development, and defense response. Cell wall polysaccharides can be broadly grouped into three categories: cellulose, pectins, and hemicelluloses. Dynamic interactions ...
    Insect Feeding Assays with Spodoptera exigua on Arabidopsis thaliana
    甜菜夜蛾对拟南芥的取食试验
    作者:Yanrong You, Chunpeng An and Chuanyou Li日期:03/05/2020,浏览量:800,Q&A: 0
    [Abstract] Plant-insect interaction is an important field for studying plant immunity. The beet armyworm, Spodoptera exigua, is one of the best-known agricultural pest insects and is usually used to study plant interactions with chewing insects. Here, we describe a protocol for insect feeding assays with Spodoptera exigua lavae using model ...
    Quantification of Protein Enrichment at Plasmodesmata
    胞间连丝富集蛋白的量化分析
    作者:Magali S. Grison, Jules D. Petit, Marie Glavier and Emmanuelle M. Bayer日期:03/05/2020,浏览量:801,Q&A: 0
    [Abstract] Intercellular communication plays a crucial role in the establishment of multicellular organisms by organizing and coordinating growth, development and defence responses. In plants, cell-to-cell communication takes place through nanometric membrane channels called plasmodesmata (PD). Understanding how PD dictate cellular connectivity greatly ...
    Tandem Tag Assay Optimized for Semi-automated in vivo Autophagic Activity Measurement in Arabidopsis thaliana roots
    优化的串联标签检测用于拟南芥根组织中体内半自动化自噬活性测定
    作者:Adrian N. Dauphinee, Jonas A. Ohlsson and Elena A. Minina日期:03/05/2020,浏览量:950,Q&A: 0
    [Abstract] Autophagy is the main catabolic process in eukaryotes and plays a key role in cell homeostasis. In vivo measurement of autophagic activity (flux) is a powerful tool for investigating the role of the pathway in organism development and stress responses. Here we describe a significant optimization of the tandem tag assay for detection of ...
    Sulfatase Assay to Determine Influence of Plants on Microbial Activity in Soil
    硫酸酯酶法测定植物对土壤微生物活性的影响
    作者:Anna Koprivova, Achim Schmalenberger and Stanislav Kopriva日期:01/20/2020,浏览量:1140,Q&A: 0
    [Abstract] Sulfatase activity is often used as a measure of the activity of soil microorganisms. It is thus a suitable tool to investigate the response of microbes to plants. Here we present a method to determine the influence of various Arabidopsis genotypes on the function of soil microbiota using the sulfatase as a quantitative measure. We grew ...
    Visualization of Actin Organization and Quantification in Fixed Arabidopsis Pollen Grains and Tubes
    固定拟南芥花粉粒及花粉管中肌动蛋白组织可视化及定量分析
    作者:Xiaolu Qu, Qiannan Wang, Haiyan Wang and Shanjin Huang日期:01/05/2020,浏览量:970,Q&A: 0
    [Abstract] Although it is widely accepted that actin plays an important role in regulating pollen germination and pollen tube growth, how actin exactly performs functions remains incompletely understood. As the function of actin is dictated by its spatial organization, it is the key to reveal how exactly actin distributes in space in pollen cells. Here we ...
    Measurement of Chloroplastic NAD Kinase Activity and Whole Tissue NAD Kinase Assay
    叶绿体NAD激酶活性及全组织NAD激酶活性测定
    作者:Yuuma Ishikawa, Maki Kawai-Yamada and Shin-nosuke Hashida日期:01/05/2020,浏览量:1195,Q&A: 0
    [Abstract] Nicotinamide adenine dinucleotide phosphate (NADP) synthesis requires nicotinamide adenine dinucleotide (NAD) kinase activity, substrate NAD and ATP. The NAD kinase responds to various environmental stimuli and its activity is regulated via various regulatory pathways, such as Ca2+-dependent and redox-dependent signals. Conventional in ...