植物

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    现刊
    RNA Purification from the Unicellular Green Alga, Chromochloris zofingiensis
    单细胞绿藻佐夫色绿藻中的RNA纯化
    作者:Sean D. Gallaher and Melissa S. Roth日期:04/05/2018,浏览量:2341,Q&A: 0
    [Abstract] Chromochloris zofingiensis is a unicellular green alga that is an emerging model species for studies in fields such as biofuel production, ketocarotenoid biosynthesis and metabolism. The recent availability of a high-quality genome assembly facilitates systems-level analysis, such as RNA-Seq. However, cells of this alga have a tough cell ...
    Fatty Acid Content and Composition of Triacylglycerols of Chlorella kessleri
    凯氏小球藻中脂肪酸含量及三酰甘油组成
    作者:Motohide Aoki and Norihiro Sato日期:01/05/2018,浏览量:3094,Q&A: 0
    [Abstract] Triacylglycerols (TAGs) are esters formed from one glycerol and three fatty acids. TAGs are induced to accumulate in algal cells under environmental stress conditions including nutrient-limitation, hyperosmosis, and low temperature, for the storage of metabolic energy and carbon, and also for the consumption of excess energy (e.g., Hirai ...
    Extraction and Molybdenum Blue-based Quantification of Total Phosphate and Polyphosphate in Parachlorella
    拟小球藻中总磷酸盐和多磷酸盐的提取和钼蓝法定量
    作者:Shuhei Ota and Shigeyuki Kawano日期:09/05/2017,浏览量:4572,Q&A: 0
    [Abstract] Inorganic phosphorus is a non-renewable resource and an essential element for life on Earth. Organisms such as algae, protists, and animals can store phosphate (Pi) through uptake of Pi as polyphosphate (poly-P), which is a linear polymer of orthophosphate residues linked by high-energy phosphoanhydride bonds. Here, we describe procedures for ...
    Extraction and Activity of O-acetylserine(thiol)lyase (OASTL) from Microalga Chlorella sorokiniana
    从小球藻Chlorella sorokiniana提取O-乙酰丝氨酸 (硫醇) 裂解酶 (OASTL) 并测定其活性
    作者:Giovanna Salbitani and Simona Carfagna日期:06/20/2017,浏览量:5156,Q&A: 0
    [Abstract] O-acetylserine(thiol)lyase (OASTL) is an enzyme catalysing the reaction of inorganic sulphide with O-acetylserine to form the S-containing amino acid L-cysteine. Here we describe an improved protocol to evaluate the activity of this enzyme from the microalga Chlorella sorokiniana. It is a colorimetric assay based on the reaction between ...
    Heat Shock Treatment of Chlamydomonas reinhardtii and Chlorella Cells
    热击处理莱茵衣藻和小球藻细胞
    作者:Stephanie Chankova, Zhana Mitrovska and Nadezhda Yurina日期:08/05/2013,浏览量:6288,Q&A: 0
    [Abstract] The protocol is very reliable and simple for inducing heat shock in unicellular green algae cells. The main purpose was to compare cellular response of three Chlorella species, isolated from different habitats: Chlorella vulgaris 8/1- thermophilic, Chlorella kesslery- mesophilic and C. vulgaris- extremophilic. ...