原核生物

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    现刊
    Probabilistic Models for Predicting Mutational Routes to New Adaptive Phenotypes
    用于预测新的适应性表型突变路径的概率模型
    作者:Eric Libby and Peter A. Lind日期:10/20/2019,浏览量:1087,Q&A: 0
    [Abstract] Understanding the translation of genetic variation to phenotypic variation is a fundamental problem in genetics and evolutionary biology. The introduction of new genetic variation through mutation can lead to new adaptive phenotypes, but the complexity of the genotype-to-phenotype map makes it challenging to predict the phenotypic effects of ...
    Cryo-transmission Electron Microscopy of Outer-inner Membrane Vesicles Naturally Secreted by Gram-negative Pathogenic Bacteria
    革兰阴性致病菌自然分泌的外-内膜囊泡低温透射电子显微镜观察
    作者:Lidia Delgado, Nicolás Baeza, Carla Pérez-Cruz, Carmen López-Iglesias and Elena Mercadé日期:09/20/2019,浏览量:1068,Q&A: 0
    [Abstract] A protocol was developed to visualize and analyze the structure of membrane vesicles (MVs) from Gram-negative bacteria. It is now accepted that these micrometric spherical vesicles are commonly produced by cells from all three domains of life, so the protocol could be useful in the study of vesicles produced by eukaryotes and archaea as well as ...
    Imaging Gene Expression Dynamics in Pseudomonas fluorescens In5 during Interactions with the Fungus Fusarium graminearum PH-1
    荧光假单胞菌In5与小麦赤霉菌PH-1互作过程中的基因表达动态成像
    作者:Rosanna C. Hennessy, Peter Stougaard and Stefan Olsson日期:06/20/2019,浏览量:1537,Q&A: 0
    [Abstract] Genomics, transcriptomics and metabolomics are powerful technologies for studying microbial interactions. The main drawback of these methods is the requirement for destructive sampling. We have established an alternative but complementary technique based on a microplate system combined with promoter fusions for visualizing gene expression in space ...
    Biofilm Formation Assay in Pseudomonas syringae
    丁香假单胞菌的生物膜形成实验
    作者:Xiaolong Shao, Yingpeng Xie, Yingchao Zhang and Xin Deng日期:05/20/2019,浏览量:3551,Q&A: 0
    [Abstract] Pseudomonas syringae is a model plant pathogen that infects more than 50 plant species worldwide, thus leading to significant yield loss. Pseudomonas biofilm always adheres to the surfaces of medical devices or host cells, thereby contributing to infection. Biofilm formation can be visualized on numerous matrixes, including ...
    An Improved Bioassay to Study Arabidopsis Induced Systemic Resistance (ISR) Against Bacterial Pathogens and Insect Pests
    一种的可用于检测拟南芥中细菌性病原体和害虫诱导的系统抗性的改进生物学方法
    作者:Nicolás M. Cecchini, Yi Song, Suruchi Roychoudhry, Jean T. Greenberg and Cara H. Haney日期:05/20/2019,浏览量:2683,Q&A: 0
    [Abstract] The plant immune system is essential for plants to perceive and defend against bacterial, fungal and insect pests and pathogens. Induced systemic resistance (ISR) is a systemic immune response that occurs upon root colonization by beneficial microbes. A well-studied model for ISR is the association of specific beneficial strains of Pseudomonas ...
    Assembly of a Custom-made Device to Study Spreading Patterns of Pseudomonas putida Biofilms
    用于恶臭假单胞菌生物膜扩散模式研究的定制设备的组装
    作者:David R. Espeso, Esteban Martínez-García and Víctor de Lorenzo日期:05/20/2019,浏览量:1720,Q&A: 0
    [Abstract] Biofilms are bacterial communities in the shape of exopolysaccharide matrix-encased aggregates attached onto interphases able to resist environmental aggressions. The development of bacteria in the shape of biofilms deeply affects the performance of many industrial processes which work with fluidic systems, where bacteria may settle and prosper. ...
    Extraction and Quantification of Polyphosphate (polyP) from Gram-negative Bacteria
    从革兰氏阴性菌中提取和测定多聚磷酸盐
    作者:Jan-Ulrik Dahl, Lihan Xie and Ursula Jakob日期:09/20/2018,浏览量:2832,Q&A: 0
    [Abstract] Polyphosphate (polyP), a universally conserved biomolecule, is composed of up to 1,000 phosphate monomers linked via phosphoanhydride bonds. Reaching levels in bacteria that are in the high nmoles per mg protein range, polyP plays important roles in biofilm formation and colonization, general stress protection and virulence. Various protocols for ...
    In planta Transcriptome Analysis of Pseudomonas syringae
    丁香假单胞菌的植物转录组分析
    作者:Tatsuya Nobori and Kenichi Tsuda日期:09/05/2018,浏览量:4352,Q&A: 0
    [Abstract] Profiling bacterial transcriptome in planta is challenging due to the low abundance of bacterial RNA in infected plant tissues. Here, we describe a protocol to profile transcriptome of a foliar bacterial pathogen, Pseudomonas syringae pv. tomato DC3000, in the leaves of Arabidopsis thaliana at an early stage of ...
    Detection of Catalase Activity by Polyacrylamide Gel Electrophoresis (PAGE) in Cell Extracts from Pseudomonas aeruginosa
    聚丙烯酰胺凝胶电泳(PAGE)检测铜绿假单胞菌细胞提取物中的过氧化氢酶活性
    作者:Magdalena Pezzoni, Ramón A. Pizarro and Cristina S. Costa日期:06/05/2018,浏览量:4295,Q&A: 0
    [Abstract] Bacteria in nature and as pathogens commonly face oxidative stress which causes damage to proteins, lipids and DNA. This damage is produced by the action of reactive oxygen species (ROS) such as hydrogen peroxide (H2O2), singlet oxygen, superoxide anion and hydroxyl radical. ROS are generated by antimicrobials, environmental ...
    Quantification of Bacterial Twitching Motility in Dense Colonies Using Transmitted Light Microscopy and Computational Image Analysis
    利用透射光学显微镜技术和计算机图像定量分析密集菌落中的细菌蹭行运动
    作者:Benjamin Smith, Jianfang Li, Matteo Metruccio, Stephanie Wan, David Evans and Suzanne Fleiszig日期:04/20/2018,浏览量:3483,Q&A: 0
    [Abstract] A method was developed to allow the quantification and mapping of relative bacterial twitching motility in dense samples, where tracking of individual bacteria was not feasible. In this approach, movies of bacterial films were acquired using differential interference contrast microscopy (DIC), and bacterial motility was then indirectly quantified ...