植物

分类

    现刊
    Live Cell Imaging of Male Meiosis in Arabidopsis by a Landmark-based System
    地标系统在拟南芥雄性减数分裂活体细胞成像中的应用
    作者:Maria Ada Prusicki, Emma Mathilde Keizer, Rik Peter van Rosmalen, Christian Fleck and Arp Schnittger日期:05/05/2020,浏览量:803,Q&A: 0
    [Abstract] Live cell imaging has tremendously promoted our understanding of cellular and subcellular processes such as cell division. Here, we present a step-by-step protocol for a robust and easy-to-use live cell imaging approach to study male meiosis in the plant Arabidopsis thaliana as recently established. Our method relies on the concomitant ...
    MAMP-triggered Medium Alkalinization of Plant Cell Cultures
    MAMP诱导植物细胞培养基质碱化
    [Abstract] Plants recognize a wide variety of microbial molecules to detect and respond to potential invaders. Recognition of Microbe-Associated Molecular Patterns (MAMPs) by cell surface receptors initiate a cascade of biochemical responses that include, among others, ion fluxes across the plasma membrane. A consequence of such event is a decrease in the ...
    Accelerated Snowmelt Protocol to Simulate Climate Change Induced Impacts on Snowpack Dependent Ecosystems
    加速雪融诱发气候变化对依赖雪被斑块生态系统的影响
    作者:Laura T. Leonard, Chelsea Wilmer, Heidi Steltzer, Kenneth H. Williams and Jonathan O. Sharp日期:03/20/2020,浏览量:686,Q&A: 0
    [Abstract] Field studies that simulate the effects of climate change are important for a predictive understanding of ecosystem responses to a changing environment. Among many concerns, regional warming can result in advanced timing of spring snowmelt in snowpack dependent ecosystems, which could lead to longer snow-free periods and drier summer soils. Past ...
    High-resolution and Deep Phylogenetic Reconstruction of Ancestral States from Large Transcriptomic Data Sets
    从大型转录组数据集中进行原始状态的高通量深度系统发育重建
    作者:Sumanth Kumar Mutte and Dolf Weijers日期:03/20/2020,浏览量:609,Q&A: 0
    [Abstract] Phylogenetics is an important area of evolutionary biology that helps to understand the origin and divergence of genes, genomes and species. Building meaningful phylogenetic trees is needed for the accurate reconstruction of the past. To achieve a correct phylogenetic understanding of genes or proteins, reliable and robust methods are needed to ...
    Experiments for in silico evaluation of Optimality of Photosynthetic Nitrogen Distribution and Partitioning in the Canopy: an Example Using Greenhouse Cucumber Plants
    树冠中光合氮分配的最适生物信息学评估实验:温室黄瓜应用实例
    作者:Yi-Chen Pao, Tsu-Wei Chen, Dany Pascal Moualeu-Ngangue and Hartmut Stützel日期:03/20/2020,浏览量:567,Q&A: 0
    [Abstract] Acclimation of leaf traits to fluctuating environments is a key mechanism to maximize fitness. One of the most important strategies in acclimation to changing light is to maintain efficient utilization of nitrogen in the photosynthetic apparatus by continuous modifications of between-leaf distribution along the canopy depth and within-leaf ...
    Single-run HPLC Quantification of Plant Cell Wall Monosaccharides
    Single-run HPLC定量分析植物细胞壁单糖
    [Abstract] The plant cell wall is a complex network of polysaccharides and proteins that provides strength and structural integrity to plant cells, as well as playing a vital role in growth, development, and defense response. Cell wall polysaccharides can be broadly grouped into three categories: cellulose, pectins, and hemicelluloses. Dynamic interactions ...
    Insect Feeding Assays with Spodoptera exigua on Arabidopsis thaliana
    甜菜夜蛾对拟南芥的取食试验
    作者:Yanrong You, Chunpeng An and Chuanyou Li日期:03/05/2020,浏览量:525,Q&A: 0
    [Abstract] Plant-insect interaction is an important field for studying plant immunity. The beet armyworm, Spodoptera exigua, is one of the best-known agricultural pest insects and is usually used to study plant interactions with chewing insects. Here, we describe a protocol for insect feeding assays with Spodoptera exigua lavae using model ...
    Quantification of Protein Enrichment at Plasmodesmata
    胞间连丝富集蛋白的量化分析
    作者:Magali S. Grison, Jules D. Petit, Marie Glavier and Emmanuelle M. Bayer日期:03/05/2020,浏览量:584,Q&A: 0
    [Abstract] Intercellular communication plays a crucial role in the establishment of multicellular organisms by organizing and coordinating growth, development and defence responses. In plants, cell-to-cell communication takes place through nanometric membrane channels called plasmodesmata (PD). Understanding how PD dictate cellular connectivity greatly ...
    Tandem Tag Assay Optimized for Semi-automated in vivo Autophagic Activity Measurement in Arabidopsis thaliana roots
    优化的串联标签检测用于拟南芥根组织中体内半自动化自噬活性测定
    作者:Adrian N. Dauphinee, Jonas A. Ohlsson and Elena A. Minina日期:03/05/2020,浏览量:705,Q&A: 0
    [Abstract] Autophagy is the main catabolic process in eukaryotes and plays a key role in cell homeostasis. In vivo measurement of autophagic activity (flux) is a powerful tool for investigating the role of the pathway in organism development and stress responses. Here we describe a significant optimization of the tandem tag assay for detection of ...
    Competition Assays to Quantify the Effect of Biocontrol Yeasts against Plant Pathogenic Fungi on Fruits
    竞争分析以量化酵母菌对水果上植物病原真菌的生防作用
    作者:Electine Magoye, Melanie Pfister, Maja Hilber-Bodmer and Florian M. Freimoser日期:02/05/2020,浏览量:546,Q&A: 0
    [Abstract] Yeasts such as Aureobasidium pullulans are unicellular fungi that occur in all environments and play important roles in biotechnology, medicine, food and beverage production, research, and agriculture. In the latter, yeasts are explored as biocontrol agents for the control of plant pathogenic fungi (e.g., Botrytis cinerea, ...