植物

分类

    现刊
    Transient Expression Assay in Strawberry Fruits
    草莓果实中的瞬时表达检测
    作者:Mengting Pi, Qi Gao and Chunying Kang日期:06/05/2019,浏览量:488,Q&A: 0
    [Abstract] Strawberry, including the woodland strawberry Fragaria vesca (2x) and the cultivated strawberry (Fragaria × ananassa, 8x), has emerged as a model system for studying fruit development and ripening. Transient expression provides a quick assay for gene functions or gene interactions. In strawberry, ...
    Simple Method to Determine Protein Redox State in Arabidopsis thaliana
    一种确定拟南芥蛋白氧化还原状态的简单方法
    作者:Keisuke Yoshida and Toru Hisabori日期:06/05/2019,浏览量:541,Q&A: 0
    [Abstract] Thiol-based redox regulation is a posttranslational protein modification that plays a key role in many biological aspects. To understand its regulatory functions, we need a method to directly assess protein redox state in vivo. Here we present a simple procedure to determine protein redox state in a model plant Arabidopsis thaliana ...
    An Adjustable Protocol to Analyze Chemical Profiles of Non-sterile Rhizosphere Soil
    一种可调整的用于分析研究非无菌根际土壤化学特性的方法
    作者:Alex Williams, Jurriaan Ton and Pierre Pétriacq日期:05/20/2019,浏览量:757,Q&A: 0
    [Abstract] The analysis of chemical diversity in non-sterile rhizosphere soil has been a pressing methodological challenge for years. Rhizosphere-enriched chemicals (i.e., rhizochemicals) include root exudation chemicals, (microbial) breakdown products thereof, and de novo produced metabolites by rhizosphere-inhabiting microbes, all of ...
    An Improved Bioassay to Study Arabidopsis Induced Systemic Resistance (ISR) Against Bacterial Pathogens and Insect Pests
    一种的可用于检测拟南芥中细菌性病原体和害虫诱导的系统抗性的改进生物学方法
    作者:Nicolás M. Cecchini, Yi Song, Suruchi Roychoudhry, Jean T. Greenberg and Cara H. Haney日期:05/20/2019,浏览量:1014,Q&A: 0
    [Abstract] The plant immune system is essential for plants to perceive and defend against bacterial, fungal and insect pests and pathogens. Induced systemic resistance (ISR) is a systemic immune response that occurs upon root colonization by beneficial microbes. A well-studied model for ISR is the association of specific beneficial strains of Pseudomonas ...
    Determination of Root Exudate Concentration in the Rhizosphere Using 13C Labeling
    13C标记测定根际根系分泌物浓度
    作者:Laurent Simon and Feth el Zahar Haichar日期:05/05/2019,浏览量:571,Q&A: 0
    [Abstract] One of the most remarkable metabolic features of plant roots is their ability to secrete a wide range of compounds into the rhizosphere, defined as the volume of soil around living roots. Around 5%-21% of total photosynthetically fixed carbon is transferred into the rhizosphere through root exudates. Until recently, studies on the quantity and ...
    Tracking Root Interactions System (TRIS) Experiment and Quality Control
    根相互作用系统的示踪实验和质量控制
    作者:Hassan Massalha, Elisa Korenblum, Orr H. Shapiro and Asaph Aharoni日期:04/20/2019,浏览量:950,Q&A: 0
    [Abstract] Soil organisms are diverse taxonomically and functionally. This ecosystem experiences highly complex networks of interactions, but may also present functionally independent entities. Plant roots, a metabolically active hotspot in the soil, take an essential part in shaping the rhizosphere. Tracking the dynamics of root-microbe interactions at high ...
    Quantification of the Humidity Effect on HR by Ion Leakage Assay
    离子渗漏实验定量分析湿度对超敏反应的影响
    作者:Musoki Mwimba and Xinnian Dong日期:04/05/2019,浏览量:621,Q&A: 0
    [Abstract] We describe a protocol to measure the contribution of humidity on cell death during the effector-triggered immunity (ETI), the plant immune response triggered by the recognition of pathogen effectors by plant resistance genes. This protocol quantifies tissue cell death by measuring ion leakage due to loss of membrane integrity during the ...
    Visualization of Plant Cell Wall Epitopes Using Immunogold Labeling for Electron Microscopy
    利用电子显微镜免疫金标记法进行植物细胞壁表位的可视化观察
    作者:Mateusz Majda日期:04/05/2019,浏览量:857,Q&A: 0
    [Abstract] Plant cell walls consist of different polysaccharides and structural proteins, which form a rigid layer located outside of the plasma membrane. The wall is also a very dynamic cell composite, which is characterized by complex polysaccharide interactions and various modifications during cell development. The visualization of cell wall components in ...
    Extraction and Purification of Laccases from Rice Stems
    水稻茎秆酶的提取与纯化
    作者:Chenna Swetha and P. V. Shivaprasad日期:04/05/2019,浏览量:589,Q&A: 0
    [Abstract] Laccases are found in cell walls of plants in very low amounts. This protocol provides an efficient method to purify laccases from rice stems. The method involves three steps: 1) Isolation of total protein from rice stems using buffers with high salt concentration to extract protein from cell walls; 2) Purification of laccases using concanavalin-A ...
    Quantitative Plasmodesmata Permeability Assay for Pavement Cells of Arabidopsis Leaves
    拟南芥叶片扁平细胞中胞间连丝渗透性定量测定实验
    作者:Min Diao, Qiannan Wang and Shanjin Huang日期:04/05/2019,浏览量:766,Q&A: 0
    [Abstract] Plasmodesmata (PD) are intercellular channels between walled plant cells that enable the transportation of materials between adjacent cells, which are important for plant growth and development. The permeability of PD must be tightly regulated. Assays to determine the permeability of PD are crucial for related studies on the regulation of PD ...