真菌

分类

    现刊
    In vitro Measurement of CMP-Sialic Acid Transporter Activity in Reconstituted Proteoliposomes
    重组蛋白脂质体中CMP-唾液酸转运体活性的体外检测
    作者:James Cahill, Shivani Ahuja and Matthew R. Whorton日期:03/20/2020,浏览量:489,Q&A: 0
    [Abstract] Nucleotide-sugar transporters (NSTs) facilitate eukaryotic cellular glycosylation by transporting nucleotide-sugar conjugates into the Golgi lumen and endoplasmic reticulum for use by glycosyltransferases, while also transferring nucleotide monophosphate byproducts to the cytoplasm. Mutations in this family of proteins can cause a number of ...
    Manganese Superoxide Dismutase Activity Assay in the Yeast Saccharomyces cerevisiae
    啤酒酵母中的锰超氧化物歧化酶活性测定
    作者:Louise Thines and Pierre Morsomme日期:03/05/2020,浏览量:597,Q&A: 0
    [Abstract] Superoxide dismutases (SODs) act as a primary defence against reactive oxygen species (ROS) by converting superoxide anion radicals (O2-) into molecular oxygen (O2) and hydrogen peroxide (H2O2). Members of this enzyme family include CuZnSODs, MnSODs, FeSODs, and NiSODs, depending on the nature ...
    Super-resolution Microscopy-based Bimolecular Fluorescence Complementation to Study Protein Complex Assembly and Co-localization
    基于高分辨率显微镜的双分子荧光互补用于蛋白复合体装配和共定位
    作者:Jingjing Chen, Zulin Yu, Jay R. Unruh, Brian D. Slaughter and Sue L. Jaspersen日期:02/20/2020,浏览量:1049,Q&A: 0
    [Abstract] Numerous experimental approaches exist to study interactions between two subunits of a large macromolecular complex. However, most methods do not provide spatial and temporal information about binding, which are critical for dissecting the mechanism of assembly of nanosized complexes in vivo. While recent advances in super-resolution ...
    Competition Assays to Quantify the Effect of Biocontrol Yeasts against Plant Pathogenic Fungi on Fruits
    竞争分析以量化酵母菌对水果上植物病原真菌的生防作用
    作者:Electine Magoye, Melanie Pfister, Maja Hilber-Bodmer and Florian M. Freimoser日期:02/05/2020,浏览量:548,Q&A: 0
    [Abstract] Yeasts such as Aureobasidium pullulans are unicellular fungi that occur in all environments and play important roles in biotechnology, medicine, food and beverage production, research, and agriculture. In the latter, yeasts are explored as biocontrol agents for the control of plant pathogenic fungi (e.g., Botrytis cinerea, ...
    Steady-state and Flux-based Trehalose Estimation as an Indicator of Carbon Flow from Gluconeogenesis or Glycolysis
    以基于稳态和通量的海藻糖测定作为糖异生或糖酵解碳流指标
    作者:Ritu Gupta and Sunil Laxman日期:01/05/2020,浏览量:991,Q&A: 0
    [Abstract] Trehalose (and glycogen) is a major storage carbohydrate in many cells, including S. cerevisiae. Typically, trehalose (a disaccharide of glucose) is synthesized and stored through gluconeogenesis. However, trehalose can also be made directly from glucose, if glucose-6-phosphate is channeled away from glycolysis or pentose phosphate ...
    Isolation and Characterization of Live Yeast Cells from Ancient Clay Vessels
    从古代陶皿中分离鉴定活酵母细胞
    [Abstract] Ancient fermented food has been studied mainly based on residue analysis and recipes and reconstruction attempts were performed using modern domesticated yeast. Furthermore, microorganisms which participated in fermentation were studied using ancient-DNA techniques. In a recent paper, we presented a novel approach based on the hypothesis that ...
    A Yeast Chromatin-enriched Fractions Purification Approach, yChEFs, from Saccharomyces cerevisiae
    酿酒酵母的染色质富集组分的纯化方法—yChEFs
    [Abstract] We have adapted a previous procedure and improved an approach that we named yChEFs (yeast Chromatin Enriched Fractions) for purifying chromatin fractions. This methodology allows the easy, reproducible and scalable recovery of proteins associated with chromatin. By using yChEFs, we bypass subcellular fractionation requirements involved when using ...
    Quantification of Mitochondrial Dynamics in Fission Yeast
    裂殖酵母内线粒体动力学的定量研究
    作者:Leeba Ann Chacko and Vaishnavi Ananthanarayanan日期:12/05/2019,浏览量:849,Q&A: 0
    [Abstract] Mitochondria are double-membraned organelles responsible for several functions in the cell including energy production, calcium signaling, and cellular metabolism. An equilibrium between fission and fusion events of mitochondria is required for their proper functioning. Mitochondrial morphologies have been quantified in yeast using image ...
    Intracellular cAMP Measurements in Candida albicans Biofilms
    白念珠菌生物膜细胞内cAMP的测定
    作者:Liuliu Jiang, Shengyan Chen, Kairui Sun, Peng Zhou and Xin Wei日期:12/05/2019,浏览量:868,Q&A: 0
    [Abstract] Candida albicans is the most common cause of fungal infections worldwide. Infection by C. albicans is closely associated with its ability to form a biofilm, closely packed communities of cells attached to the surfaces of human tissues and implanted devices, in or on the host. When tested for susceptibility to antifungals, such as ...
    Imaging Cryptococcus spp. Capsule by Differential Interference Contrast Microscopy Using Percoll®
    利用 Percoll®在微分干涉显微镜下对隐球菌荚膜成像
    [Abstract] The most important virulence factor in the Cryptococcus genus is the polysaccharide capsule. This genus includes several species that cause life-threatening invasive disease. An increase in capsule thickness is important during fungal infection. The capsule is usually imaged using India ink, and crucial insights on the dynamics of its ...