植物科学

分类

    现刊
    Extraction of RNA from Recalcitrant Tree Species Paulownia elongata
    从顽拗型树种泡桐中提取RNA
    作者:Niveditha Ramadoss and Chhandak Basu日期:07/20/2018,浏览量:1640,Q&A: 0
    [Abstract] Isolation of pure RNA is the basic requisite for most molecular biology work. Plants contain polyphenols and polysaccharides, which can interfere with isolation of pure RNA from them. Especially hardwood tree species like Paulownia elongata have surplus amount of RNA-binding alkaloids, proteins and secondary metabolites that can further ...
    RNA Purification from the Unicellular Green Alga, Chromochloris zofingiensis
    单细胞绿藻佐夫色绿藻中的RNA纯化
    作者:Sean D. Gallaher and Melissa S. Roth日期:04/05/2018,浏览量:2132,Q&A: 0
    [Abstract] Chromochloris zofingiensis is a unicellular green alga that is an emerging model species for studies in fields such as biofuel production, ketocarotenoid biosynthesis and metabolism. The recent availability of a high-quality genome assembly facilitates systems-level analysis, such as RNA-Seq. However, cells of this alga have a tough cell ...
    Isolation of Nuclei in Tagged Cell Types (INTACT), RNA Extraction and Ribosomal RNA Degradation to Prepare Material for RNA-Seq
    从标记的细胞类型中分离细胞核、提取RNA并去除核糖体RNA以用于RNA-Seq 分析
    [Abstract] Gene expression is dynamically regulated on many levels, including chromatin accessibility and transcription. In order to study these nuclear regulatory events, we describe our method to purify nuclei with Isolation of Nuclei in TAgged Cell Types (INTACT). As nuclear RNA is low in polyadenylated transcripts and conventional pulldown methods would ...
    Total RNA Extraction from Grape Berry Skin for Quantitative Reverse Transcription PCR and Microarray Analysis
    从葡萄果皮上提取总RNA进行定量反转录PCR和基因芯片分析
    作者:Mami Suzuki and Katsuhiro Shiratake日期:04/05/2016,浏览量:4722,Q&A: 0
    [Abstract] Extraction of high quality RNA is an essential step for quantitative reverse transcription PCR (qRT-PCR) and microarray analysis. However, it is not easy to extract high quality RNA from fruit materials, which contain high amounts of polysaccharides, lipids and secondary metabolites. Wan and Wilkins (1994) had developed ‘Hot Borate Method’ to ...
    Isolation of Polysome-bound mRNA from Rice Solid Tissues Amenable for RT-PCR and Profiling Experiments
    分离水稻固体组织的多核糖体结合mRNA用于RT-PCR和图谱实验
    作者:Mehdi Jabnoune, David Secco, Cécile Lecampion, Christophe Robaglia, Qingyao Shu and Yves Poirier日期:03/05/2015,浏览量:9243,Q&A: 0
    [Abstract] Polysome profile analysis is a frequently performed task in translational control research that not only enables direct monitoring of the efficiency of translation but can easily be extended with a wide range of downstream applications such as Northern and western blotting, genome-wide microarray analysis or qRT-PCR. Here, we describe a method for ...
    Polysomal-mRNA Extraction from Arabidopsis by Sucrose-gradient Separation
    采用甘蔗梯度分离法提取拟南芥多聚核糖体-mRNA
    作者:Shih-Yun Lin and Guang-Yuh Jauh日期:12/20/2014,浏览量:7359,Q&A: 0
    [Abstract] mRNAs surrounded by polysomes are ready for translation into proteins (Warner et al., 1963); these mRNAs are defined as polysomal-mRNAs (Mustroph et al., 2009). The process is affected by various growth conditions or surrounding situations. Microarray analysis is a powerful tool for detecting genome-wide gene expression. ...
    RNA Extraction from RNase-Rich Senescing Leaf Samples
    一种从富含RNA酶的衰老叶片样本中提取RNA的方法
    作者:Susheng Gan日期:08/20/2012,浏览量:8153,Q&A: 0
    [Abstract] Isolation of intact, full-length high quality RNAs is essential for RNA sequencing, reverse transcription PCR analysis of gene expression as well as RNA gel blot analysis. This simple yet easy protocol is developed to meet this need; in addition to regular samples, this protocol is especially good for isolating RNAs from RNase-rich samples such ...