微生物学

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    现刊
    I Plate-based Assay for Studying How Fungal Volatile Compounds (VCs) Affect Plant Growth and Development and the Identification of VCs via SPME-GC-MS
    用于研究真菌挥发性化合物(VCs)如何影响植物生长发育并通过SPME-GC-MS技术鉴定VCs的I板检测法
    作者:Wenzhao Wang, Ningxiao Li, Xingzhong Liu and Seogchan Kang日期:02/20/2019,浏览量:2907,Q&A: 0
    [Abstract] Biogenic volatile compounds (VCs) mediate various types of crucial intra- and inter-species interactions in plants, animals, and microorganisms owing to their ability to travel through air, liquid, and porous soils. To study how VCs produced by Verticillium dahliae, a soilborne fungal pathogen, affect plant growth and development, we ...
    Soluble and Solid Iron Reduction Assays with Desulfitobacterium hafniense
    哈夫尼脱亚硫酸杆菌用于可溶和固体铁的还原试验
    作者:Lucrezia Comensoli, Julien Maillard, Wafa M. Kooli, Pilar Junier and Edith Joseph日期:09/05/2018,浏览量:2449,Q&A: 0
    [Abstract] There is a pressing need to develop sustainable and efficient methods to protect and stabilize iron objects. To develop a conservation-restoration method for corroded iron objects, this bio-protocol presents the steps to investigate reductive dissolution of ferric iron and biogenic production of stabilizing ferrous iron minerals in the strict ...
    Quantification of Bacterial Polyhydroxybutyrate Content by Flow Cytometry
    流式细胞术定量分析细菌聚羟基丁酸酯含量
    作者:Antonio Lagares (Jr.) and Claudio Valverde日期:12/05/2017,浏览量:4183,Q&A: 0
    [Abstract] We describe here a detailed protocol for the quantification of the intracellular content of polyhydroxybutyrate (PHB) in a population of bacterial cells by flow cytometry, which is based on a consensus of several previously reported works.
    A Protocol of Using White/Red Color Assay to Measure Amyloid-induced Oxidative Stress in Saccharomyces cerevisiae
    测定酿酒酵母中淀粉样蛋白诱导氧化应激的白/红色实验方案
    作者:Vidhya Bharathi, Amandeep Girdhar and Basant K Patel日期:08/05/2017,浏览量:6705,Q&A: 0
    [Abstract] The yeast Saccharomyces cerevisiae (S. cerevisiae) harboring ade1 or ade2 mutations manifest red colony color phenotype on rich yeast medium YPD. In these mutants, intermediate metabolites of adenine biosynthesis pathway are accumulated. Accumulated intermediates, in the presence of reduced glutathione, are ...
    Measuring Cyanobacterial Metabolism in Biofilms with NanoSIMS Isotope Imaging and Scanning Electron Microscopy (SEM)
    利用纳米SIMS同位素成像和扫描电子显微镜(SEM)测量蓝藻生物膜中的代谢
    作者:Rhona K. Stuart, Xavier Mayali, Michael P. Thelen, Jennifer Pett-Ridge and Peter K. Weber日期:05/05/2017,浏览量:5632,Q&A: 0
    [Abstract] To advance the understanding of microbial interactions, it is becoming increasingly important to resolve the individual metabolic contributions of microorganisms in complex communities. Organisms from biofilms can be especially difficult to separate, image and analyze, and methods to address these limitations are needed. High resolution imaging ...
    Antibiotic Disc Assay for Synechocystis sp. PCC6803
    采用抗生素纸盘试验法测定集胞藻PCC6803的细胞壁功能
    作者:Otilia Cheregi and Christiane Funk日期:12/20/2016,浏览量:6668,Q&A: 0
    [Abstract] This protocol describes how to investigate the integrity of the outer cell wall in the cyanobacterium Synechocystis sp. PCC6803 using antibiotics. It is adapted to the agar diffusion test (Bauer et al., 1966), in which filter paper discs impregnated with specified concentrations of antibiotics were placed on agar plates ...
    Pyocyanin Extraction and Quantitative Analysis in Swarming Pseudomonas aeruginosa
    群集铜绿假单胞菌中的绿脓菌素提取和定量分析
    作者:Michelle M. King, Manita Guragain, Svetlana A. Sarkisova and Marianna A. Patrauchan日期:12/05/2016,浏览量:9980,Q&A: 0
    [Abstract] This protocol describes the quantification of pyocyanin extracted from swarming colonies of Pseudomonas aeruginosa. Pyocyanin is a secondary metabolite and a major virulence factor, whose production is inducible and varies highly under different growth conditions. The protocol is based on the earlier developed chloroform/HCl extraction of ...
    59Fe Uptake Assays in Paracoccidioides Species
    副球孢子菌属的59Fe摄取实验
    [Abstract] Iron is an essential micronutrient required for virtually all organisms. This fact is related to the ability of the transition metal to exist in two oxidation states, the reduced ferrous (Fe2+) and the oxidized ferric (Fe3+). Given the relative availability of aqueous iron (the element which constitutes ~5% of the earth’s ...
    A Modified Chromogenic Assay for Determination of the Ratio of Free Intracellular NAD+/NADH in Streptococcus mutans
    改进型显色试验测定变异链球菌中的游离胞内NAD+/NADH比率
    作者:Jonathon L. Baker, Roberta C. Faustoferri and Robert G. Quivey, Jr日期:08/20/2016,浏览量:8566,Q&A: 2
    [Abstract] Nicotinamide adenine dinucleotide is a coenzyme present in all kingdoms of life and exists in two forms: oxidized (NAD+) and reduced (NADH). NAD(H) is involved in a multitude of essential metabolic redox reactions, providing oxidizing or reducing equivalents. The ratio of free intracellular NAD+/NADH is fundamentally ...
    Fluorescent Detection of Intracellular Nitric Oxide in Staphylococcus aureus
    荧光检测葡萄球菌属中的胞内一氧化氮
    作者:April M. Lewis, Silvia S. Matzdorf and Kelly C. Rice日期:07/20/2016,浏览量:6016,Q&A: 0
    [Abstract] Nitric Oxide (NO) is a highly-reactive radical gas that can modify a variety of cellular targets in both eukaryotes and bacteria. NO is produced endogenously by a wide variety of organisms: For example, as a cell-signaling molecule in mammals and bacteria via nitric oxide synthase (NOS) enzymes, and as a product of denitrification. As such, it is ...