细胞生物学

分类

    现刊
    Production and Bioassay of a Diffusible Factor that Induces Gametophyte-to-Sporophyte Developmental Reprogramming in the Brown Alga Ectocarpus
    水云属褐藻配子体-孢子体发育重编程扩散因子的产生及生物测定
    [Abstract] The brown alga Ectocarpus has a haploid-diploid life cycle that involves alternation between two multicellular generations, the sporophyte and the gametophyte. Life cycle generation is not determined by ploidy but by a genetic system that includes two different three amino acid loop extension homeodomain transcription factors called ...
    Organotypic Slice Culture of the Embryonic Mouse Brain
    胚胎小鼠大脑的器官型切片培养
    作者:James M. Clegg and Thomas Pratt日期:07/05/2020,浏览量:619,Q&A: 0
    [Abstract] Organotypic slice culture is a powerful technique for exploring the embryonic development of the mammalian brain. In this protocol we describe a basic slice culture technique we have used for two sets of experiments: axon guidance transplant assays and bead culture assays.
    Quantitative Live-cell Reporter Assay for Noncanonical Wnt Activity
    可定量的活细胞报告基因用于检测非经典Wnt活动
    作者:Edith P. Karuna, Michael W. Susman and Hsin-Yi Henry Ho日期:03/20/2018,浏览量:5316,Q&A: 0
    [Abstract] Noncanonical Wnt signaling functions independently of the β-catenin pathway to control diverse developmental processes, and dysfunction of the pathway contributes to a number of human pathological conditions, including birth defects and metastatic cancer. Progress in the field, however, has been hampered by the scarcity of functional assays for ...
    1-MCP (1-methylcyclopropene) Treatment Protocol for Fruit or Vegetables
    水果或蔬菜的1-MCP(1-甲基环丙烯)处理实验方案
    作者:Gamrasni Dan, Goldway Martin, Stern Yosi, Breitel Dario and Aharoni Asaph日期:05/20/2017,浏览量:11311,Q&A: 4
    [Abstract] 1-MCP (1-methylcyclopropene) is a simple synthetic hydrocarbon molecule that interacts with the ethylene receptor and inhibits the response of fruit or plant to ethylene. 1-MCP has opened new opportunities in handling harvested crops and serves as a powerful tool to learn about plant response to ethylene (Watkins and Miller, 2006). 1-MCP is ...
    Detection of Wnt5 in Media Conditioned by Mouse Embryonic Fibroblast
    检测小鼠胚胎成纤维细胞调节培养基中的Wnt5
    作者:Juan Flores and Nan Gao日期:10/20/2016,浏览量:6039,Q&A: 0
    [Abstract] This protocol describes the procedure of visualizing secreted Wnt5 protein in serum free media via western blotting. This procedure can also be used to visualize other secreted proteins larger than 10,000 daltons. The work presented in this paper visualizes Wnt5 secreted by mouse embryonic fibroblast (MEF), but can be adapted to other cell lines ...
    TGFβ Release Co-culture Assay
    TGFβ 释放共培养试验
    作者:Emanuel Rognoni日期:12/05/2014,浏览量:8613,Q&A: 0
    [Abstract] TGFβ is a potent cytokine modulating various processes including proliferation, differentiation, ECM synthesis and apoptosis (Siegel and Massague, 2003). Thus in many tissues availability of TGFβ is tightly regulated. TGFβ is secreted as an inactive complex where it is encapsulated by the latency associated protein (LAP), a ligand trap protein, ...
    Separation of the Inner and Outer Mitochondrial Membrane in HeLa Cells
    HeLa细胞中线粒体内外膜的分离
    作者:Naotaka Nishimura and Masato Yano日期:11/20/2014,浏览量:20763,Q&A: 2
    [Abstract] Mitochondria are organelles that have important functions in oxidative phosphorylation, fatty acid oxidation and apoptosis signaling. They have two distinct membranes, outer membrane (OM) and inner membrane (IM). IM contains respiratory chain complexes that produce ATP. IM is rich in cardiolipin, a specific phospholipid reportedly having a ...
    Wnt Reporter Activity Assay
    Wnt基因活性报告实验
    作者:Chen Zhao日期:07/20/2014,浏览量:26681,Q&A: 2
    [Abstract] This protocol is for testing responses of a candidate cell line/cell lines to Wnt ligands or Wnt pathway agonists stimulation. This protocol can also be adapted to screen small molecule libraries or biologics that contain activities to either increase or decrease Wnt pathway responses. Canonical Wnt signaling activity transcriptionally induces Wnt ...
    Hedgehog (Hh) Reporter Activity Assay
    Hedgehog基因活性报告实验
    作者:Chen Zhao日期:07/20/2014,浏览量:11917,Q&A: 0
    [Abstract] This protocol is for testing responses of a candidate cell line/cell lines to Hh ligands or Hh pathway agonists stimulation. This protocol can also be adapted to screen small molecule libraries or biologics that contain activities to either increase or decrease Hh pathway responses. Canonical Hh signaling activity transcriptionally induces Hh ...