微生物学

分类

    现刊
    Quantification of Bacteria Residing in Caenorhabditis elegans Intestine
    秀丽隐杆线虫肠道细菌数量测定
    作者:M. Fernanda Palominos and Andrea Calixto日期:05/05/2020,浏览量:659,Q&A: 0
    [Abstract] Quantification of intestinal colonization by pathogenic or commensal bacteria constitute a critical part of the analysis to understand host-microbe interactions during different time points of their interplay. Here we detail a method to isolate non-pathogenic and pathogenic bacteria from C. elegans intestines, and classify gut phenotypes ...
    Zebrafish Bacterial Infection Assay to Study Host-Pathogen Interactions
    斑马鱼细菌感染检测用于研究宿主-病原相互作用
    作者:Faiza Basheer, Clifford Liongue and Alister C Ward日期:03/05/2020,浏览量:511,Q&A: 0
    [Abstract] The study of host-pathogen interactions has improved our understanding of both pathogenesis and the response of the host to infection, including both innate and adaptive responses. Neutrophils and macrophages represent the first line of innate host defense against any infection. The zebrafish is an ideal model to study the response of these cells ...
    Transcervical Mouse Infections with Chlamydia trachomatis and Determination of Bacterial Burden
    小鼠宫颈沙眼衣原体感染及细菌负荷测定
    作者:Karthika Rajeeve and Rajeeve Sivadasan日期:02/05/2020,浏览量:808,Q&A: 0
    [Abstract] Chlamydia trachomatis is an obligate human pathogen. It infects the genital tract of humans ascending into the fallopian tube, exacerbated by chronic pelvic pain, pelvic inflammatory disease, and fallopian tube scaring resulting in infertility and other malignancies. The major hurdle in controlling chlamydial spread is that the ...
    Study of Microbial Extracellular Vesicles: Separation by Density Gradients, Protection Assays and Labelling for Live Tracking
    微生物胞外囊泡的研究:密度梯度分离,保护性分析和活体示踪标记
    作者:Carolina Coelho, Raghav Vij, Daniel Q. Smith, Nathan R. Brady, Anne Hamacher-Brady and Arturo Casadevall日期:01/20/2020,浏览量:686,Q&A: 0
    [Abstract] Extracellular vesicles (EVs) are produced by all domains of life including Bacteria, Archaea and Eukarya. EVs are critical for cellular physiology and contain varied cargo: virulence factors, cell wall remodeling enzymes, extracellular matrix components and even nucleic acids and metabolites. While various protocols for isolating EVs have been ...
    A Microbial Bioassay for Direct Contact Assessment of Soil Toxicity Based on Oxygen Consumption of Sulfur Oxidizing Bacteria
    基于硫氧化细菌耗氧的直接接触土壤毒性的微生物检定方法
    作者:Ebenezer Ashun, Umair Ali Toor, Heonseop Eom and Sang-Eun Oh日期:01/05/2020,浏览量:630,Q&A: 0
    [Abstract] A new direct contact assessment of soil toxicity using sulfur oxidizing bacteria (SOB) is proposed for analyzing the toxicity of soils. The proposed method is based on the ability of SOB to oxidize elemental sulfur to sulfuric acid in the presence of oxygen. Since sulfate ions are produced from sulfur by SOB oxidation activity, changes in ...
    Propagation and Purification of Chlamydia trachomatis Serovar L2 Transformants and Mutants
    沙眼衣原体血清型L2转化子和突变体的繁殖及纯化
    作者:Robert Faris and Mary M. Weber日期:12/20/2019,浏览量:741,Q&A: 0
    [Abstract] Chlamydia trachomatis (C.t.) is an obligate intracellular pathogen that cannot be cultured axenically and must be propagated within eukaryotic host cells. There are at least 15 distinct chlamydial serovariants that belong to 2 major biovars commonly referred to as trachoma and lymphogranuloma venereum (LGV). The invasive ...
    Lysosome Targeting RedGreen-assay: Selective Autophagy Sensing Assay for Mammalian Cells
    溶酶体靶向红绿试验
    作者:Victoria Riccio, Miluska Vissa, Angus McQuibban and Peter Kijun Kim日期:12/20/2019,浏览量:1181,Q&A: 0
    [Abstract] The process of autophagy is an essential cellular mechanism, required to maintain general cell health through the removal of dysfunctional organelles, such as the ER, peroxisomes and mitochondria, as well as protein aggregates, and bacteria. Autophagy is an extremely dynamic process, and tools are constantly being developed to study the various ...
    Assessing Different Ways of Bacillus subtilis Spreading over Abiotic Surfaces
    枯草芽孢杆菌在非生物表面传播途径的不同检测方法
    作者:Marco Bartolini and Roberto Grau日期:11/20/2019,浏览量:1100,Q&A: 0
    [Abstract] Surface-associate motility on biotic and abiotic environments is a key mechanism used by the model bacterium Bacillus subtilis and its closest relatives (i.e., B. amyloliquefaciens, B. thuringiensis, B. cereus, B. pumilus) for surface colonization and spreading across surfaces. The study of ...
    Plaque Assay to Determine Invasion and Intercellular Dissemination of Shigella flexneri in TC7 Human Intestinal Epithelial Cells
    蚀斑测定弗氏志贺在TC7人肠上皮细胞中的侵袭和细胞间传播
    作者:Atin Sharma and Andrea Puhar日期:07/05/2019,浏览量:1394,Q&A: 0
    [Abstract] Shigella flexneri invades the epithelial cells lining the gut lumen and replicates intracellularly. The specialized Type III Secretion System (T3SS) and its effector proteins, encoded on a large virulence plasmid, assist the bacterium to gain access to the cytosol. Thereafter Shigella disseminates to neighboring cells in an ...
    Gentamicin Protection Assay to Determine the Number of Intracellular Bacteria during Infection of Human TC7 Intestinal Epithelial Cells by Shigella flexneri
    庆大霉素保护试验确定福氏志贺菌侵染人TC7肠上皮细胞的胞内寄生数目
    作者:Atin Sharma and Andrea Puhar日期:07/05/2019,浏览量:2233,Q&A: 0
    [Abstract] Shigella flexneri is an intracellular bacterial pathogen that gains access to the gut epithelium using a specialized Type III Secretion System (T3SS). Various determinants mediating this invasive infection have been experimentally verified using the classical gentamicin protection assay presented here. In this assay epithelial cell lines ...