植物科学

分类

    现刊
    Increasing the Membrane Permeability of a Fern with DMSO
    使用DMSO增加膜蕨类的膜通透性
    作者:Marcelo Garcés日期:06/20/2018,浏览量:1721,Q&A: 0
    [Abstract] Cell membrane prevents the entrance of extra molecules (e.g., transcription and translation inhibitors) into the cell. For studying the physiological effects of transcription and translation inhibitors on Hymenophyllum caudiculatum fronds, we incubate fronds with 0.1% DMSO to test if this increases cell membrane permeability ...
    Slow and Fast Desiccation of Single-cell Thick Fronds of Filmy Ferns
    膜蕨类植物单细胞厚叶状体的缓慢及快速干燥
    作者:Marcelo Garcés日期:06/05/2018,浏览量:1456,Q&A: 0
    [Abstract] Filmy ferns can desiccate and recover after rehydration to resume photosynthesis. Slow and fast desiccation rates were compared in filmy fern fronds to determine whether structural or physiological differences may occur between desiccation rates. Slow desiccation is considered to be more similar to natural conditions experienced by plants that ...
    Fluorescein Transport Assay to Assess Bulk Flow of Molecules Through the Hypocotyl in Arabidopsis thaliana
    荧光素转运测定法评估通过拟南芥下胚轴的分子总体流动
    作者:Salva Duran-Nebreda and George W. Bassel日期:04/05/2018,浏览量:2221,Q&A: 0
    [Abstract] The bulk transport of molecules through plant tissues underpins growth and development. The stem acts as a conduit between the upper and low domains of the plant, facilitating transport of solutes and water from the roots to the shoot system, and sugar plus other elaborated metabolites towards the non-photosynthetic organs. In order to perform ...
    Electron Tomography to Study the Three-dimensional Structure of Plasmodesmata in Plant Tissues–from High Pressure Freezing Preparation to Ultrathin Section Collection
    采用电子断层摄影术研究植物组织中的胞间连丝的三维结构 — 从高压冷冻制备到超薄切片采集
    作者:William J. Nicolas, Emmanuelle Bayer and Lysiane Brocard日期:01/05/2018,浏览量:3485,Q&A: 0
    [Abstract] Plasmodesmata (PD) are nanometric (~20 nm wide) membrane lined pores encased in the cell walls of the adjacent plant cells. They allow the cells to exchange all types of molecules ranging from nutrients like sugar, hormones, to RNAs and various proteins. Unfortunately, they are also hijacked by phyto-viruses, enabling them to spread from ...
    Preparation of Onion Epidermal Cell Walls for Imaging by Atomic Force Microscopy (AFM)
    制备洋葱表皮细胞壁用于原子力显微镜(AFM)成像
    作者:Tian Zhang and Daniel J. Cosgrove日期:12/20/2017,浏览量:4976,Q&A: 0
    [Abstract] The growing plant cell wall is comprised of long, thin cellulose microfibrils embedded in a hydrated matrix of polysaccharides and glycoproteins. These components are typically constructed in layers (lamellae) on the inner surface of the cell wall, i.e., between the existing wall and the plasma membrane. The organization of these ...
    Automatic Leaf Epidermis Assessment Using Fourier Descriptors in Texture Images
    在纹理图像中使用傅立叶描述符以进行自动叶表皮评估
    [Abstract] The identification of plant species is not a trivial task, since it is carried out by experts and depends on the presence of fruits, flowers and leaves. However, fruits and flowers are not available throughout the year, while leaves are accessible most of the year. In order to assist the specialized work of species identification, methods of ...
    Histochemical Preparations to Depict the Structure of Cauliflower Leaf Hydathodes
    用于研究花椰菜叶泌水孔结构的组织化学制备
    作者:Aude Cerutti, Marie-Christine Auriac, Laurent D. Noël and Alain Jauneau日期:10/20/2017,浏览量:3986,Q&A: 0
    [Abstract] Hydathodes are plant organs present on leaf margins of a wide range of vascular plants and are the sites of guttation. Both anatomy and physiology of hydathodes are poorly documented. We have recently reported on the anatomy of cauliflower and Arabidopsis thaliana hydathodes and on their infection by the vascular pathogenic bacterium ...
    Capturing Z-stacked Confocal Images of Living Bacteria Entering Hydathode Pores of Cauliflower
    采集活细菌进入花椰菜泌水孔的Z-栈共聚焦图像
    作者:Aude Cerutti and Alain Jauneau日期:10/20/2017,浏览量:4218,Q&A: 0
    [Abstract] The present protocol to visualize living bacteria at the pore level of cauliflower hydathodes is simple and trained users in confocal microscopy can execute it successfully. It can be easily adapted to capture images with other plant-microorganism interactions at the leaf surface and should be useful to obtain important information on pore and ...
    Scanning Electron Microscopy of Motile Male Gametes of Land Plants
    陆生植物游动雄配子的扫描电子显微镜观察
    作者:Karen Sue Renzaglia, Renee A. Lopez and Steven J. Schmitt日期:10/05/2017,浏览量:3621,Q&A: 0
    [Abstract] The only motile cells produced in land plants are male gametes (spermatozoids), which are reduced to non-flagellated cells in flowering plants and most gymnosperms. Although a coiled architecture is universal, the complexity of land plant flagellated cells varies from biflagellated in bryophytes to thousands of flagella per gametes in the seed ...
    Transmission Electron Microscopy of Centrioles, Basal Bodies and Flagella in Motile Male Gametes of Land Plants
    陆生植物游动雄配子中心粒、基体和鞭毛的透射电子显微镜观察
    作者:Karen Sue Renzaglia, Renee A. Lopez, Jason S. Henry, Nicholas D. Flowers and Kevin C. Vaughn日期:10/05/2017,浏览量:3601,Q&A: 0
    [Abstract] Motile male gametes (spermatozoids) of land plants are coiled and contain a modified and precisely organized complement of organelles that includes a locomotory apparatus with two to thousands of flagella. Each flagellum is generated from a basal body that originates de novo as a centriole in spermatogenous cell lineages. Much of what is ...