微生物学

分类

    现刊
    Bacterial Cell Wall Precursor Phosphatase Assays Using Thin-layer Chromatography (TLC) and High Pressure Liquid Chromatography (HPLC)
    使用薄层色谱法(TLC)和高压液相色谱法(HPLC)进行细菌细胞壁前体磷酸酶测定
    作者:Manuel Pazos, Christian Otten and Waldemar Vollmer日期:03/20/2018,浏览量:2281,Q&A: 0
    [Abstract] Peptidoglycan encases the bacterial cytoplasmic membrane to protect the cell from lysis due to the turgor. The final steps of peptidoglycan synthesis require a membrane-anchored substrate called lipid II, in which the peptidoglycan subunit is linked to the carrier lipid undecaprenol via a pyrophosphate moiety. Lipid II is the target of ...
    Fatty Acid Content and Composition of Triacylglycerols of Chlorella kessleri
    凯氏小球藻中脂肪酸含量及三酰甘油组成
    作者:Motohide Aoki and Norihiro Sato日期:01/05/2018,浏览量:3073,Q&A: 0
    [Abstract] Triacylglycerols (TAGs) are esters formed from one glycerol and three fatty acids. TAGs are induced to accumulate in algal cells under environmental stress conditions including nutrient-limitation, hyperosmosis, and low temperature, for the storage of metabolic energy and carbon, and also for the consumption of excess energy (e.g., Hirai ...
    Isolation, BODIPY Labeling and Uptake of Exosomes in Hepatic Stellate Cells
    肝星状细胞中外泌体的分离、BODIPY标记和摄取
    作者:Shubham Shrivastava日期:12/05/2017,浏览量:4409,Q&A: 1
    [Abstract] Exosomes have emerged as an important mediator of intercellular communication. They are present in extracellular milieu and therefore, easily accessible by neighboring or distant cells. They carry mRNA, microRNAs and proteins within their vesicles and once internalized by recipient cells; they can modulate multiple signaling pathways with ...
    Crude Preparation of Lipopolysaccharide from Helicobacter pylori for Silver Staining and Western Blot
    粗制备幽门螺杆菌脂多糖用于银染和蛋白质印迹
    作者:Hong Li and Mohammed Benghezal日期:10/20/2017,浏览量:4525,Q&A: 0
    [Abstract] This protocol provides an easy and rapid method to prepare lipopolysaccharide from the gastric pathogen Helicobacter pylori for visualization on acrylamide gels by silver staining and for detecting the presence of Lewis antigens by Western blot. The silver staining is a four-step procedure, involving a 20 min-oxidation step, a 10 ...
    Liposome Disruption Assay to Examine Lytic Properties of Biomolecules
    采用脂质体破碎测定法检测生物分子的裂解性质
    作者:John R. Jimah, Paul H. Schlesinger and Niraj H. Tolia日期:08/05/2017,浏览量:6074,Q&A: 0
    [Abstract] Proteins may have three dimensional structural or amino acid features that suggest a role in targeting and disrupting lipids within cell membranes. It is often necessary to experimentally investigate if these proteins and biomolecules are able to disrupt membranes in order to conclusively characterize the function of these biomolecules. Here, we ...
    Loading of Extracellular Vesicles with Chemically Stabilized Hydrophobic siRNAs for the Treatment of Disease in the Central Nervous System
    将化学稳定的疏水siRNA加载到细胞外囊泡中用于治疗中枢神经系统疾病
    作者:Reka A. Haraszti, Andrew Coles, Neil Aronin, Anastasia Khvorova and Marie-Cécile Didiot日期:06/20/2017,浏览量:4400,Q&A: 0
    [Abstract] Efficient delivery of oligonucleotide therapeutics, i.e., siRNAs, to the central nervous system represents a significant barrier to their clinical advancement for the treatment of neurological disorders. Small, endogenous extracellular vesicles were shown to be able to transport lipids, proteins and RNA between cells, including neurons. ...
    Preparation of Everted Membrane Vesicles from Escherichia coli Cells
    利用大肠埃希杆菌细胞制备反转膜囊泡
    作者:Marina Verkhovskaya日期:05/05/2017,浏览量:4193,Q&A: 0
    [Abstract] The protocol for obtaining electrically sealed membrane vesicles from E. coli cells is presented. Proton pumps such as Complex I, quinol oxidase, and ATPase are active in the obtained vesicles. Quality of the preparation was tested by monitoring the electric potential generated by these pumps.
    Procedure for Rhamnolipids Quantification Using Methylene-blue
    使用亚甲蓝进行鼠李糖脂量化的方法
    作者:Tsiry Rasamiravaka, Olivier M. Vandeputte and Mondher El Jaziri日期:04/05/2016,浏览量:8483,Q&A: 2
    [Abstract] Rhamnolipids produced by Pseudomonas aeruginosa (P. aeruginosa) represent a group of biosurfactants with various applications (e.g., bioremediation of oil spills, cosmetics, detergents and cleaners). The commonly used colorimetric methods for rhamnolipid quantification, including anthrone, phenol−sulfuric acid and ...
    [14C] Linoleic Acid Uptake and Fractionation Assay in Vibrio cholerae
    霍乱弧菌中[14C] 亚油酸的摄取和分级分离试验
    作者:Sarah C. Plecha and Jeffrey H. Withey日期:12/20/2015,浏览量:4143,Q&A: 0
    [Abstract] The gram-negative curved bacillus Vibrio cholerae (V. cholerae) causes the severe diarrheal illness cholera. The work presented here is to assess whether unsaturated fatty acids (UFAs), such as linoleic acid, have the potential to directly affect proteins involved in DNA binding because they are able to enter the cell. In this ...
    Extraction and Purification of Mycobacterial Mycolic Acids
    分枝杆菌霉菌酸的提取和纯化
    作者:Christian M. Dupont and Laurent Kremer日期:10/20/2014,浏览量:8828,Q&A: 0
    [Abstract] Mycolic acids are major long-chain fatty acids, containing up to 80-90 carbon atoms that represent essential components of the mycobacterial cell wall (Pawelczyk and Kremer, 2014). Each mycobacterial species possesses a specific mycolic acid profile characterized by various chemical modifications that decorate the lipid. Mycolic acids play a ...