分子生物学

分类

    现刊
    Circadian Gene Profiling in Laser Capture Microdissected Mouse Club Cells
    激光捕获显微解剖小鼠棒状细胞的昼夜节律基因分析
    作者:Zhenguang Zhang and Andrew Loudon日期:04/20/2020,浏览量:400,Q&A: 0
    [Abstract] Cell heterogeneity is high in tissues like lung. Research conducted on pure population of cells usually offers more insights than bulk tissues, such as circadian clock work. In this protocol, we provide a detailed work flow on how to do circadian clock study by RNA seq in laser capture micro-dissected mouse lung club cells. The method uses frozen ...
    mRNA Extraction from Gill Tissue for RNA-sequencing
    鳃组织mRNA 提取用于RNA测序
    作者:Jukka-Pekka Verta and Felicity Jones日期:03/05/2020,浏览量:515,Q&A: 0
    [Abstract] Adaptation is thought to proceed in part through spatial and temporal changes in gene expression. Fish species such as the threespine stickleback are powerful vertebrate models to study the genetic architecture of adaptive changes in gene expression since divergent adaptation to different environments is common, they are abundant and easy to study ...
    RNA Sequencing of Single Myofibers from Mus musculus
    小鼠单肌纤维的RNA序列分析
    作者:Darren M. Blackburn, Felicia Lazure and Vahab D. Soleimani日期:02/20/2020,浏览量:1379,Q&A: 0
    [Abstract] Whole transcriptome analysis is a key method in biology that allows researchers to determine the effect a condition has on gene regulation. One difficulty in RNA sequencing of muscle is that traditional methods are performed on the whole muscle, but this captures non-myogenic cells that are part of the muscle. In order to analyze only the ...
    Structural Alignment and Covariation Analysis of RNA Sequences
    RNA序列的结构比对和协变分析
    作者:Nicolas J. Tourasse and Fabien Darfeuille日期:02/05/2020,浏览量:679,Q&A: 0
    [Abstract] RNA molecules adopt defined structural conformations that are essential to exert their function. During the course of evolution, the structure of a given RNA can be maintained via compensatory base-pair changes that occur among covarying nucleotides in paired regions. Therefore, for comparative, structural, and evolutionary studies of RNA ...
    Application of a Modified Smart-seq2 Sample Preparation Protocol for Rare Cell Full-length Single-cell mRNA Sequencing to Mouse Oocytes
    一种改良的Smart-seq2样品制备方案在小鼠卵母细胞中应用稀有细胞全长单细胞mRNA测序
    作者:Rebecca S. Treger, Scott D. Pope, Xiaojun Xing and Akiko Iwasaki日期:08/20/2019,浏览量:1564,Q&A: 0
    [Abstract] Endogenous retroviruses (ERV) are transposable retroelements that form ~10% of the murine genome and whose family members are differentially expressed throughout embryogenesis. However, precise regulation of ERV in germ cells remains unclear. To investigate ERV expression in oocytes, we adapted a single-cell mRNA-sequencing library preparation ...
    Axon-seq for in Depth Analysis of the RNA Content of Neuronal Processes
    神经元突起RNA含量的Axon-seq 深入分析
    作者:Jik Nijssen, Julio Aguila and Eva Hedlund日期:07/20/2019,浏览量:2946,Q&A: 0
    [Abstract] Neuronal processes have an RNA composition that is distinct from the cell body. Therefore, to fully understand neuronal biology in health and disease we need to study both somas, dendrites and axons. Here we describe a detailed protocol of a newly refined method, Axon-seq, for RNA sequencing of axons (and dendrites) grown in isolation using single ...
    Enterovirus Competition Assay to Assess Replication Fitness
    用于检测复制适应性的肠道病毒竞争实验
    作者:Valeria Lulla and Andrew E. Firth日期:05/20/2019,浏览量:1979,Q&A: 0
    [Abstract] In virology the difference between the fitness of two viruses can be determined by using various methods, such as virus titer, growth curve analysis, measurement of virus infectivity, analysis of produced RNA copies and viral protein production. However, for closely performing viruses, it is often very hard to distinguish the differences. In vitro ...
    Preparation of RNA 3’ End Sequencing Libraries of Total and 4-thiouracil Labeled RNA for Simultaneous Measurement of Transcription, RNA Synthesis and Decay in S. cerevisiae
    制备酿酒酵母总RNA和4-硫尿RNA的RNA3'端测序文库用于转录,RNA合成和降解的同步测定
    作者:Manfred Schmid, Agnieszka Tudek and Torben Heick Jensen日期:03/20/2019,浏览量:1990,Q&A: 0
    [Abstract] Cellular RNA levels are determined by the rates of RNA transcription from the gene template and subsequent RNA stability. Knowledge about both transcription and RNA decay is, therefore, necessary to interpret RNA levels and gene expression, especially during cellular processes where these parameters change. Numerous experimental strategies have ...
    QsRNA-seq: A protocol for Generating Libraries for High-throughput Sequencing of Small RNAs
    QsRNA-seq: 小RNA高通量测序文库构建方案
    作者:Alla Fishman and Ayelet T. Lamm日期:03/05/2019,浏览量:2747,Q&A: 0
    [Abstract] Small RNAs (sRNAs) are 20-30 nt long non-coding RNA molecules that regulate essentially all cellular processes. Besides being an intensively studied topic in academic research, sRNAs also hold a promise as clinical biomarkers. While the need for expressional profiling of sRNAs is growing, preparation of sRNA libraries for high-throughput ...
    Gene Mapping by RNA-sequencing: A Direct Way to Characterize Genes and Gene Expression through Targeted Queries of Large Public Databases
    通过RNA测序进行基因定位:一种通过大型公共数据库目标查询来鉴定基因和基因表达的直接方法
    作者:Peter Rotwein日期:01/05/2019,浏览量:2469,Q&A: 0
    [Abstract] Recent advances in genomics present new opportunities for enhancing knowledge about gene regulation and function across a wide spectrum of organisms and species. Understanding and evaluating this information at the individual gene level is challenging, and not only requires extracting, collating and interpreting data from public genetic ...