分子生物学

分类

    现刊
    Axon-seq for in Depth Analysis of the RNA Content of Neuronal Processes
    神经元突起RNA含量的Axon-seq 深入分析
    作者:Jik Nijssen, Julio Aguila and Eva Hedlund日期:07/20/2019,浏览量:40,Q&A: 0
    [Abstract] Neuronal processes have an RNA composition that is distinct from the cell body. Therefore, to fully understand neuronal biology in health and disease we need to study both somas, dendrites and axons. Here we describe a detailed protocol of a newly refined method, Axon-seq, for RNA sequencing of axons (and dendrites) grown in isolation using single ...
    Enterovirus Competition Assay to Assess Replication Fitness
    用于检测复制适应性的肠道病毒竞争实验
    作者:Valeria Lulla and Andrew E. Firth日期:05/20/2019,浏览量:393,Q&A: 0
    [Abstract] In virology the difference between the fitness of two viruses can be determined by using various methods, such as virus titer, growth curve analysis, measurement of virus infectivity, analysis of produced RNA copies and viral protein production. However, for closely performing viruses, it is often very hard to distinguish the differences. In vitro ...
    Preparation of RNA 3’ End Sequencing Libraries of Total and 4-thiouracil Labeled RNA for Simultaneous Measurement of Transcription, RNA Synthesis and Decay in S. cerevisiae
    制备酿酒酵母总RNA和4-硫尿RNA的RNA3'端测序文库用于转录,RNA合成和降解的同步测定
    作者:Manfred Schmid, Agnieszka Tudek and Torben Heick Jensen日期:03/20/2019,浏览量:775,Q&A: 0
    [Abstract] Cellular RNA levels are determined by the rates of RNA transcription from the gene template and subsequent RNA stability. Knowledge about both transcription and RNA decay is, therefore, necessary to interpret RNA levels and gene expression, especially during cellular processes where these parameters change. Numerous experimental strategies have ...
    QsRNA-seq: A protocol for Generating Libraries for High-throughput Sequencing of Small RNAs
    QsRNA-seq: 小RNA高通量测序文库构建方案
    作者:Alla Fishman and Ayelet T. Lamm日期:03/05/2019,浏览量:1059,Q&A: 0
    [Abstract] Small RNAs (sRNAs) are 20-30 nt long non-coding RNA molecules that regulate essentially all cellular processes. Besides being an intensively studied topic in academic research, sRNAs also hold a promise as clinical biomarkers. While the need for expressional profiling of sRNAs is growing, preparation of sRNA libraries for high-throughput ...
    Gene Mapping by RNA-sequencing: A Direct Way to Characterize Genes and Gene Expression through Targeted Queries of Large Public Databases
    通过RNA测序进行基因定位:一种通过大型公共数据库目标查询来鉴定基因和基因表达的直接方法
    作者:Peter Rotwein日期:01/05/2019,浏览量:1107,Q&A: 0
    [Abstract] Recent advances in genomics present new opportunities for enhancing knowledge about gene regulation and function across a wide spectrum of organisms and species. Understanding and evaluating this information at the individual gene level is challenging, and not only requires extracting, collating and interpreting data from public genetic ...
    Preparation of Sequencing RNA Libraries through Chemical Cross-linking Coupled to Affinity Purification (cCLAP) in Saccharomyces cerevisiae
    通过化学交联联合亲和纯化(cCLAP) 技术制备酿酒酵母RNA测序文库
    作者:Congwei Wang, Julie Weidner and Anne Spang日期:10/05/2018,浏览量:1837,Q&A: 0
    [Abstract] Ribonucleoprotein particles (mRNPs) are complexes consisting of mRNAs and RNA-binding proteins (RBPs) which control mRNA transcription localization, turnover, and translation. Some mRNAs within the mRNPs have been shown to undergo degradation or storage. Those transcripts can lack general mRNA elements, like the poly(A) tail or 5’ cap structure, ...
    In planta Transcriptome Analysis of Pseudomonas syringae
    丁香假单胞菌的植物转录组分析
    作者:Tatsuya Nobori and Kenichi Tsuda日期:09/05/2018,浏览量:2447,Q&A: 0
    [Abstract] Profiling bacterial transcriptome in planta is challenging due to the low abundance of bacterial RNA in infected plant tissues. Here, we describe a protocol to profile transcriptome of a foliar bacterial pathogen, Pseudomonas syringae pv. tomato DC3000, in the leaves of Arabidopsis thaliana at an early stage of ...
    Characterizing the Transcriptional Effects of Endolysin Treatment on Established Biofilms of Staphylococcus aureus
    鉴定内溶素处理对已建立的金黄色葡萄球菌生物膜转录的影响
    [Abstract] Biofilms are the most common lifestyle of bacteria in both natural and human environments. The organized structure of these multicellular communities generally protects bacterial cells from external challenges, thereby enhancing their ability to survive treatment with antibiotics or disinfectants. For this reason, the search for new antibiofilm ...
    A Modified Low-quantity RNA-Seq Method for Microbial Community and Diversity Analysis Using Small Subunit Ribosomal RNA
    改良的低量 RNA-Seq法通过检测小亚基核糖体RNA以分析微生物群落和多样性
    作者:Yong-Wei Yan, Ting Zhu, Bin Zou and Zhe-Xue Quan日期:05/05/2018,浏览量:1665,Q&A: 0
    [Abstract] We propose a modified RNA-Seq method for small subunit ribosomal RNA (SSU rRNA)-based microbial community analysis that depends on the direct ligation of a 5’ adaptor to RNA before reverse-transcription. The method requires only a low-input quantity of RNA (10-100 ng) and does not require a DNA removal step. Using this method, we could obtain more ...
    Isolation of Nuclei in Tagged Cell Types (INTACT), RNA Extraction and Ribosomal RNA Degradation to Prepare Material for RNA-Seq
    从标记的细胞类型中分离细胞核、提取RNA并去除核糖体RNA以用于RNA-Seq 分析
    [Abstract] Gene expression is dynamically regulated on many levels, including chromatin accessibility and transcription. In order to study these nuclear regulatory events, we describe our method to purify nuclei with Isolation of Nuclei in TAgged Cell Types (INTACT). As nuclear RNA is low in polyadenylated transcripts and conventional pulldown methods would ...