植物科学

分类

    现刊
    MAMP-triggered Medium Alkalinization of Plant Cell Cultures
    MAMP诱导植物细胞培养基质碱化
    [Abstract] Plants recognize a wide variety of microbial molecules to detect and respond to potential invaders. Recognition of Microbe-Associated Molecular Patterns (MAMPs) by cell surface receptors initiate a cascade of biochemical responses that include, among others, ion fluxes across the plasma membrane. A consequence of such event is a decrease in the ...
    An Adjustable Protocol to Analyze Chemical Profiles of Non-sterile Rhizosphere Soil
    一种可调整的用于分析研究非无菌根际土壤化学特性的方法
    作者:Alex Williams, Jurriaan Ton and Pierre Pétriacq日期:05/20/2019,浏览量:2875,Q&A: 0
    [Abstract] The analysis of chemical diversity in non-sterile rhizosphere soil has been a pressing methodological challenge for years. Rhizosphere-enriched chemicals (i.e., rhizochemicals) include root exudation chemicals, (microbial) breakdown products thereof, and de novo produced metabolites by rhizosphere-inhabiting microbes, all of ...
    An Inexpensive and Comprehensive Method to Examine and Quantify Field Insect Community Influenced by Host Plant Olfactory Cues
    一种分析量化寄主植物嗅觉引诱性物质对田间昆虫群落影响的低成本、综合方法
    作者:Rupesh Kariyat, Jesus Chavana and Jasleen Kaur日期:08/20/2018,浏览量:3035,Q&A: 0
    [Abstract] Insect pollinators, herbivores and their natural enemies use olfactory cues emitted by their host plants to locate them. In insect-plant ecology, understanding the mechanisms underlying these interactions are of critical importance, as this bio-communication has both ecological and agricultural applications. However, the first step in such ...
    Analysis of Metals in Whole Cells, Thylakoids and Photosynthetic Protein Complexes in Synechocystis sp. PCC6803
    分析集胞藻属PCC6803全细胞、类囊体和光合蛋白复合体中的金属
    作者:Chiara Gandini, Søren Husted and Sidsel Birkelund Schmidt日期:06/20/2018,浏览量:2901,Q&A: 0
    [Abstract] Metals are essential in many biological processes, including oxygenic photosynthesis. Here we described a method to measure the metal pool in whole cells and thylakoids, including the bioactive pool in intact photosynthetic protein complexes in the model oxygenic cyanobacterium Synechocystis PCC6803. In the first part of the protocol, ...
    In vitro Nitrate Reductase Activity Assay from Arabidopsis Crude Extracts
    拟南芥粗提物中硝酸还原酶体外活性测定
    作者:Joo Yong Kim and Hak Soo Seo日期:04/05/2018,浏览量:6135,Q&A: 0
    [Abstract] Nitrate reductase (NR) reduces the major plant nitrogen source, NO3-, into NO2-. NR activity can be measured by its final product, nitrite through its absorbance under optimized condition. Here, we present a detailed protocol for measuring relative enzyme activity of NR from Arabidopsis crude ...
    Estimation of Silica Cell Silicification Level in Grass Leaves Using in situ Charring Method
    使用原位炭化法估算草叶中二氧化硅细胞的硅化水平
    作者:Santosh Kumar and Rivka Elbaum日期:11/20/2017,浏览量:4603,Q&A: 0
    [Abstract] Silica cells are specialized leaf epidermal cells in grasses with almost the whole cell volume filled with solid silica. In sorghum, silica deposition in silica cells takes place in young, elongating leaves around the mid-length of the leaf. We developed a protocol for estimating the level of silica cell silicification in Sorghum bicolor ...
    Extraction and Molybdenum Blue-based Quantification of Total Phosphate and Polyphosphate in Parachlorella
    拟小球藻中总磷酸盐和多磷酸盐的提取和钼蓝法定量
    作者:Shuhei Ota and Shigeyuki Kawano日期:09/05/2017,浏览量:6484,Q&A: 0
    [Abstract] Inorganic phosphorus is a non-renewable resource and an essential element for life on Earth. Organisms such as algae, protists, and animals can store phosphate (Pi) through uptake of Pi as polyphosphate (poly-P), which is a linear polymer of orthophosphate residues linked by high-energy phosphoanhydride bonds. Here, we describe procedures for ...
    Determination of Reduced and Total Glutathione Content in Extremophilic Microalga Galdieria phlegrea
    极端环境微藻Galdieria phlegrea中还原型和总谷胱甘肽含量的测定
    作者:Giovanna Salbitani, Claudia Bottone and Simona Carfagna日期:07/05/2017,浏览量:12995,Q&A: 2
    [Abstract] Glutathione is an important molecule involved in the primary and secondary metabolism of all organisms. The Glutathione redox status is an indicator of the cellular redox state. Therefore, it is important to have precise methods on hand to determine the glutathione redox status in the cell. In this protocol, we describe an improved ...
    Acetyl Bromide Soluble Lignin (ABSL) Assay for Total Lignin Quantification from Plant Biomass
    采用乙酰溴可溶性木质素(ABSL)测定法定量植物生物量中的总木质素
    作者:William J. Barnes and Charles T. Anderson日期:03/05/2017,浏览量:10625,Q&A: 0
    [Abstract] Lignin is the second most abundant biopolymer on Earth, providing plants with mechanical support in secondary cell walls and defense against abiotic and biotic stresses. However, lignin also acts as a barrier to biomass saccharification for biofuel generation (Carroll and Somerville, 2009; Zhao and Dixon, 2011; Wang et al., 2013). For ...
    Rice Root Organic Acid Efflux Measurement by Using Ion Chromatography
    采用离子色谱法测定水稻根有机酸分泌
    作者:Chun-quan Zhu, Xiao-fang Zhu and Ren-fang Shen日期:02/20/2017,浏览量:5843,Q&A: 0
    [Abstract] Organic acids secreted from plant roots play important roles in various biological processes including nutrient acquisition, metal detoxification, and pathogen attraction. The secretion of organic acids may be affected by various conditions such as plant growth stage, nutrient deficiency, and abiotic stress. For example, when white lupin (Lupinus ...