分子生物学

分类

    现刊
    BRIDGE: An Open Platform for Reproducible Protein-Ligand Simulations and Free Energy of Binding Calculations
    BRIDGE:一个可重复的蛋白质配体模拟和结合自由能计算的开放平台
    作者:Tharindu Senapathi, Christopher B. Barnett and Kevin J. Naidoo日期:09/05/2020,浏览量:788,Q&A: 0
    [Abstract] Protein-ligand binding prediction is central to the drug-discovery process. This often follows an analysis of genomics data for protein targets and then protein structure discovery. However, the complexity of performing reproducible protein conformational analysis and ligand binding calculations, using vetted methods and protocols can be a ...
    Microtubule Seeded-assembly in the Presence of Poorly Nucleating Nucleotide Analogues
    存在不良成核核苷酸类似物的微管种晶组装
    作者:Siou Ku, Claire Heichette, Laurence Duchesne and Denis Chrétien日期:08/20/2020,浏览量:472,Q&A: 0
    [Abstract] Microtubule dynamic instability is driven by the hydrolysis of the GTP bound to the β-subunit of the α-β tubulin heterodimer. Nucleotide analogues are commonly used to mimic the different steps of the tubulin GTPase cycle, but most of them are poor microtubule nucleators. Usually, microtubule assembly is seeded by guanylyl-(α, ...
    Generation and Testing of Fluorescent Adaptable Simple Theranostic (FAST) Proteins
    FAST蛋白的生成与检测
    [Abstract] This protocol provides a step-by-step method to create recombinant fluorescent fusion proteins that can be secreted from mammalian cell lines. This builds on many other recombinant protein and fluorescent protein techniques, but is among the first to harness fluorescent fusion proteins secreted directly into cell culture supernatant. This opens ...
    In vitro Assessment of Pathogen Effector Binding to Host Proteins by Surface Plasmon Resonance
    利用表面等离子体共振技术评价病原菌与宿主蛋白的结合
    作者:Marina Franceschetti, Mark J. Banfield, Clare E. M. Stevenson and Juan Carlos De la Concepcion日期:07/05/2020,浏览量:1146,Q&A: 0
    [Abstract] The mechanisms of virulence and immunity are often governed by molecular interactions between pathogens and host proteins. The study of these interactions has major implications on understanding virulence activities, and how the host immune system recognizes the presence of pathogens to initiate an immune response. Frequently, the association ...
    Molecular Size Analysis of Recombinant Importin-histone Complexes Using Analytical Ultracentrifugation
    重组输入蛋白-组蛋白配合物分子大小的超离心分析
    作者:Abhilash Padavannil, Chad A. Brautigam and Yuh Min Chook日期:05/20/2020,浏览量:941,Q&A: 0
    [Abstract] Histones constitute the protein components of nucleosomes. Despite their small sizes, histones do not diffuse through the nuclear pore complex. Instead, they are transported to the nucleus by importins, either alone or in complex with histone chaperones. Determining the molecular size of the importin-histone complexes is key to understanding the ...
    Generation, Analyzing and in-vivo Drug Treatment of Drosophila Models with IBMPFD
    果蝇IBMPFD模型的建立、分析及体内药物治疗
    作者:Ting Zhang, Bruce A. Hay and Ming Guo日期:05/20/2020,浏览量:929,Q&A: 0
    [Abstract] Missense mutations of p97/cdc48/Valosin-containing protein (VCP) cause inclusion body myopathy, Paget disease with frontotemporal dementia (IBMPFD) and other neurodegenerative diseases. The pathological mechanism of IBMPFD is not clear and there is no treatment. We generated Drosophila models of IBMPFD in adult flight muscle in ...
    Split Nano Luciferase-based Assay to Measure Assembly of Japanese Encephalitis Virus
    基于分割纳米荧光素酶的乙型脑炎病毒组装检测
    作者:Simon Goto, Kotaro Ishida, Ryosuke Suzuki and Eiji Morita日期:05/05/2020,浏览量:656,Q&A: 0
    [Abstract] Cells infected with flavivirus release various forms of infectious and non-infectious particles as products and by-products. Comprehensive profiling of the released particles by density gradient centrifugation is informative for understanding viral particle assembly. However, it is difficult to detect low-abundance minor particles in such ...
    Bimolecular Fluorescence Complementation (BiFC) for Studying Sarcomeric Protein Interactions in Drosophila
    双分子荧光互补法研究果蝇肌节蛋白相互作用
    作者:Océane Marescal, Frieder Schöck and Nicanor González-Morales日期:04/05/2020,浏览量:1237,Q&A: 0
    [Abstract] Protein-protein interactions in Drosophila myofibrils are essential for their function and formation. Bimolecular Fluorescence Complementation (BiFC) is an effective method for studying protein interactions and localization. BiFC relies on the reconstitution of a monomeric fluorescent protein from two half-fragments when in proximity. Two ...
    Super-resolution Microscopy-based Bimolecular Fluorescence Complementation to Study Protein Complex Assembly and Co-localization
    基于高分辨率显微镜的双分子荧光互补用于蛋白复合体装配和共定位
    作者:Jingjing Chen, Zulin Yu, Jay R. Unruh, Brian D. Slaughter and Sue L. Jaspersen日期:02/20/2020,浏览量:1566,Q&A: 0
    [Abstract] Numerous experimental approaches exist to study interactions between two subunits of a large macromolecular complex. However, most methods do not provide spatial and temporal information about binding, which are critical for dissecting the mechanism of assembly of nanosized complexes in vivo. While recent advances in super-resolution ...
    Assessing Self-interaction of Mammalian Nuclear Proteins by Co-immunoprecipitation
    共免疫沉淀法评价哺乳动物核蛋白的自身相互作用
    作者:Claudia Cattoglio, Iryna Pustova, Xavier Darzacq, Robert Tjian and Anders S. Hansen日期:02/20/2020,浏览量:1781,Q&A: 0
    [Abstract] Protein-protein interactions constitute the molecular foundations of virtually all biological processes. Co-immunoprecipitation (CoIP) experiments are probably the most widely used method to probe both heterotypic and homotypic protein-protein interactions. Recent advances in super-resolution microscopy have revealed that several nuclear proteins ...