生物化学

分类

    现刊
    Gas Chromatography Detection Protocol of Short-chain Fatty Acids in Mice Feces
    小鼠粪便中短链脂肪酸的气相色谱检测
    作者:Chaozheng Zhang, An Liu, Tianshuang Zhang, Yi Li and Hua Zhao日期:07/05/2020,浏览量:115,Q&A: 0
    [Abstract] Short-chain fatty acids (SCFAs), which are formed mainly by bacteria fermenting undigested carbohydrates in the colon, they are based on the number of carbon atoms in the carbon chain. Organic fatty acids with less than 6 carbon atoms are called short-chain fatty acids. SCFAs are closely related to various aspects of the human body, so more and ...
    Pancreatic Acinar Cell Preparation for Oxygen Consumption and Lactate Production Analysis
    用于耗氧量和乳酸生产分析的胰腺腺泡细胞制备
    作者:Jane A. Armstrong, Robert Sutton and David N. Criddle日期:05/20/2020,浏览量:567,Q&A: 0
    [Abstract] Mitochondrial dysfunction is a principal feature of acute pancreatitis (AP) although the underlying mechanisms are still unclear. AP precipitants induce Ca2+-dependent formation of the mitochondrial permeability transition pore (MPTP) in pancreatic acinar cells (PACs), leading to ATP depletion and necrosis. Evaluations of mitochondrial ...
    Quantification of Fatty Acids in Mammalian Tissues by Gas Chromatography–Hydrogen Flame Ionization Detection
    气相色谱-氢火焰离子化检测法测定哺乳动物组织中的脂肪酸
    作者:Fumie Hamano, Suzumi M Tokuoka, Tomomi Hashidate-Yoshida, Hideo Shindou, Takao Shimizu and Yoshihiro Kita日期:05/05/2020,浏览量:820,Q&A: 0
    [Abstract] In mammalian organisms, fatty acids (FAs) exist mostly in esterified forms, as building blocks of phospholipids, triglycerides, and cholesteryl esters, while some exist as non-esterified free FAs. The absolute quantification of FA species in total lipids or in a specific lipid class is critical in lipid-metabolism studies. To quantify FAs in ...
    In vivo Quantification of Alkanes in Escherichia coli
    大肠杆菌中脂肪醛去甲酰加氧酶产生烷烃的体内量化
    作者:Tabinda Shakeel, Zia Fatma and Syed Shams Yazdani日期:04/20/2020,浏览量:840,Q&A: 0
    [Abstract] Microbial production of alkanes employing synthetic biology tools has gained tremendous attention owing to the high energy density and similarity of alkanes to existing petroleum fuels. One of the most commonly studied pathways includes the production of alkanes by AAR (acyl-ACP (acyl carrier protein) reductase)-ADO (aldehyde deformylating ...
    Quantitative Determination of Ca2+-binding to Ca2+-sensor Proteins by Isothermal Titration Calorimetry
    钙离子与钙离子传感器蛋白结合的等温滴定量热法定量测定
    作者:Seher Abbas and Karl-Wilhelm Koch日期:04/05/2020,浏览量:619,Q&A: 0
    [Abstract] Diverse and complex molecular recognitions are central elements of signal transduction cascades. The strength and nature of these interaction modes can be determined by different experimental approaches. Among those, Isothermal titration calorimetry (ITC) offers certain advantages by providing binding constants and thermodynamic parameters from ...
    Shared Pheromonal Communication of Specific Fear Between Adult Sprague Dawley Rats
    成年SD大鼠特定恐惧的信息素通讯
    作者:Samantha J. Carew and Abhinaba Ghosh日期:03/20/2020,浏览量:521,Q&A: 0
    [Abstract] Rats are highly social animals, and mainly communicate with one another in two ways: through ultrasonic vocalizations and pheromones. Most research on pheromones has been dedicated those regarding sexual behavior, but more recently pheromones which signal danger to conspecifics have been identified in rodents. In fact, rats are capable of ...
    Live Mitochondrial or Cytosolic Calcium Imaging Using Genetically-encoded Cameleon Indicator in Mammalian Cells
    利用基因编码Cameleon指示剂进行哺乳动物细胞中活体线粒体或胞质钙成像
    作者:Elisa Greotti and Tullio Pozzan日期:02/05/2020,浏览量:1237,Q&A: 0
    [Abstract] Calcium (Ca2+) imaging aims at investigating the dynamic changes in live cells of its concentration ([Ca2+]) in different pathophysiological conditions. Ca2+ is an ubiquitous and versatile intracellular signal that modulates a large variety of cellular functions thanks to a cell type-specific toolkit and a complex ...
    Measurement of Chloroplastic NAD Kinase Activity and Whole Tissue NAD Kinase Assay
    叶绿体NAD激酶活性及全组织NAD激酶活性测定
    作者:Yuuma Ishikawa, Maki Kawai-Yamada and Shin-nosuke Hashida日期:01/05/2020,浏览量:1160,Q&A: 0
    [Abstract] Nicotinamide adenine dinucleotide phosphate (NADP) synthesis requires nicotinamide adenine dinucleotide (NAD) kinase activity, substrate NAD and ATP. The NAD kinase responds to various environmental stimuli and its activity is regulated via various regulatory pathways, such as Ca2+-dependent and redox-dependent signals. Conventional in ...
    Intracellular cAMP Measurements in Candida albicans Biofilms
    白念珠菌生物膜细胞内cAMP的测定
    作者:Liuliu Jiang, Shengyan Chen, Kairui Sun, Peng Zhou and Xin Wei日期:12/05/2019,浏览量:1071,Q&A: 0
    [Abstract] Candida albicans is the most common cause of fungal infections worldwide. Infection by C. albicans is closely associated with its ability to form a biofilm, closely packed communities of cells attached to the surfaces of human tissues and implanted devices, in or on the host. When tested for susceptibility to antifungals, such as ...
    Amplex Red Assay for Measuring Hydrogen Peroxide Production from Caenorhabditis elegans
    利用Amplex Red检测秀丽隐杆线虫中过氧化氢的生成
    作者:Ozgur Karakuzu, Melissa R. Cruz, Yi Liu and Danielle A. Garsin日期:11/05/2019,浏览量:2291,Q&A: 0
    [Abstract] Reagents such as Amplex® Red have been developed for detecting hydrogen peroxide (H2O2) and are used to measure the release of H2O2 from biological samples such as mammalian leukocytes undergoing the oxidative burst. Caenorhabditis elegans is commonly used as a model host in the study ...