微生物学

分类

    现刊
    Visualizing Hypoxia in a Murine Model of Candida albicans Infection Using in vivo Biofluorencence
    用活体生物荧光法观察小鼠白念珠菌感染模型中的缺氧情况
    作者:José Pedro Lopes and Constantin F. Urban日期:08/05/2019,浏览量:272,Q&A: 0
    [Abstract] Candida albicans is a leading human fungal pathogen that uses several metabolic adaptations to escape immune cells and causes systemic disease. Here, we describe a protocol for measuring one of these adaptations, the ability to thrive in hypoxic niches. Hypoxia was generated after successful subdermal infection with C. albicans ...
    Image-based Quantification of Direct Cell-to-cell Transmission of Bovine Viral Diarrhea Virus
    基于成像量化分析牛病毒性腹泻病毒的细胞间传播
    作者:Fernando Merwaiss and Diego E. Alvarez日期:08/05/2019,浏览量:175,Q&A: 0
    [Abstract] Different viruses rely on direct cell-to-cell transmission to propagate infection within the infected host. Measuring this mode of transmission in cultured cells is often complicated by the contribution of cell free viruses to spread, and the difficulty to distinguish between primary infected cells that produce the virus and neighboring cells that ...
    Endocytosis Detection in Magnaporthe oryzae
    稻瘟病菌内吞作用的检测
    作者:Muxing Liu and Zhengguang Zhang日期:08/05/2019,浏览量:181,Q&A: 0
    [Abstract] Endocytosis is an intracellular trafficking pathway that occurs in nutrient uptake, signal transduction and reconstruction of cell polarity and is conserved in eukaryotic cells. In fungi, endocytosis plays crucial roles in the physiology of hyphal growth and pathogenicity. vidence for endocytosis in filamentous fungi is detected by the ...
    Quantification of Blumenol Derivatives as Leaf Biomarkers for Plant-AMF Association
    蓝甲醇衍生物作为丛枝菌根真菌与植物共生标志物的定量研究
    作者:Elisabeth Mindt, Ming Wang, Martin Schäfer, Rayko Halitschke and Ian T. Baldwin日期:07/20/2019,浏览量:605,Q&A: 0
    [Abstract] Symbiotic interactions between arbuscular mycorrhizal fungi (AMF) and plants are widespread among land plants and can be beneficial for both partners. The plant is provided with mineral nutrients such as nitrogen and phosphorous, whereas it provides carbon resources for the fungus in return. Due to the large economic and environmental impact, ...
    Isolation of Powdery Mildew Haustoria from Infected Barley
    感染大麦中白粉病吸器的分离提取
    作者:Linhan Li, Benjamin Collier and Pietro D. Spanu日期:07/20/2019,浏览量:555,Q&A: 0
    [Abstract] Blumeria graminis is a fungus that causes powdery mildews on grasses, such as barley. Investigations of this pathogen present many challenges due to its obligate biotrophic nature. This means that the fungus can only grow in the presence of a living host plant. B. graminis forms epiphytic mycelia on the plant surface and feeding ...
    Plaque Assay to Determine Invasion and Intercellular Dissemination of Shigella flexneri in TC7 Human Intestinal Epithelial Cells
    蚀斑测定弗氏志贺在TC7人肠上皮细胞中的侵袭和细胞间传播
    作者:Atin Sharma and Andrea Puhar日期:07/05/2019,浏览量:436,Q&A: 0
    [Abstract] Shigella flexneri invades the epithelial cells lining the gut lumen and replicates intracellularly. The specialized Type III Secretion System (T3SS) and its effector proteins, encoded on a large virulence plasmid, assist the bacterium to gain access to the cytosol. Thereafter Shigella disseminates to neighboring cells in an ...
    Gentamicin Protection Assay to Determine the Number of Intracellular Bacteria during Infection of Human TC7 Intestinal Epithelial Cells by Shigella flexneri
    庆大霉素保护试验确定福氏志贺菌侵染人TC7肠上皮细胞的胞内寄生数目
    作者:Atin Sharma and Andrea Puhar日期:07/05/2019,浏览量:513,Q&A: 0
    [Abstract] Shigella flexneri is an intracellular bacterial pathogen that gains access to the gut epithelium using a specialized Type III Secretion System (T3SS). Various determinants mediating this invasive infection have been experimentally verified using the classical gentamicin protection assay presented here. In this assay epithelial cell lines ...
    Fluorescence Microscopy Assay to Measure HIV-1 Capsid Uncoating Kinetics in vitro
    HIV-1衣壳脱壳动力学的荧光显微体外检测
    [Abstract] The stability of the HIV-1 capsid and the spatiotemporal control of its disassembly, a process called uncoating, need to be finely tuned for infection to proceed. Biochemical methods for measuring capsid lattice disassembly in bulk are unable to resolve intermediates in the uncoating reaction. We have developed a single-particle fluorescence ...
    In vitro Infection of Primary Human Monocytes with HIV-1
    原代人单核细胞的体外HIV-1侵染
    作者:Patrick O’Connell, Yong-Hui Zheng, Andrea Amalfitano and Yasser A. Aldhamen日期:07/05/2019,浏览量:488,Q&A: 0
    [Abstract] Monocyte infection by HIV-1 is an important component of chronic HIV pathogenesis. Following infection by HIV-1, monocytes are able to cross the blood brain barrier and set up a viral reservoir in the central nervous system. Additionally, in the setting of chronic HIV-1 infection, monocytes can become activated either directly through HIV-1 ...
    Analysis of Functional Virus-generated PAMP RNAs Using IFNα/β ELISA Assay
    利用干扰素α/β酶联免疫吸附试验检测分析病毒产生的功能性PAMP RNAs
    作者:Margit Mutso, Xiang Liu, Andres Merits and Suresh Mahalingam日期:06/20/2019,浏览量:428,Q&A: 0
    [Abstract] Virus-generated PAMP RNAs are key factors that activate host immune response. The PAMP RNAs are therefore usually closely related with viral disease pathogenesis. Quantitative real time PCR is a conventional method to assess RNA. However, it cannot be used for detecting short dsRNAs generated by viral replicase. This protocol was established to ...