系统生物学

分类

    现刊
    Delayed Alternation Task for the Study of Spatial Working and Long Term Memory in Rats
    延迟交替任务用于研究大鼠空间工作记忆和长期记忆
    作者:Megi Hoxha and Marta Sabariego日期:03/05/2020,浏览量:950,Q&A: 0
    [Abstract] Memory systems can hold previously presented information for several seconds, bridging gaps between discontinuous events. It has been previously demonstrated that the hippocampus and the medial entorhinal cortex (mEC) are necessary for memory retention over delay intervals in alternation tasks. Here we describe the delayed alternation task, a ...
    ChIP-Seq from Limited Starting Material of K562 Cells and Drosophila Neuroblasts Using Tagmentation Assisted Fragmentation Approach
    用Tagmentation Assisted Fragmentation法从K562细胞和果蝇神经母细胞的有限起始材料中提取芯片序列
    作者:Junaid Akhtar, Piyush More and Steffen Albrecht日期:02/20/2020,浏览量:700,Q&A: 0
    [Abstract] Chromatin immunoprecipitation is extensively used to investigate the epigenetic profile and transcription factor binding sites in the genome. However, when the starting material is limited, the conventional ChIP-Seq approach cannot be implemented. This protocol describes a method that can be used to generate the chromatin profiles from as low as ...
    Probabilistic Models for Predicting Mutational Routes to New Adaptive Phenotypes
    用于预测新的适应性表型突变路径的概率模型
    作者:Eric Libby and Peter A. Lind日期:10/20/2019,浏览量:1198,Q&A: 0
    [Abstract] Understanding the translation of genetic variation to phenotypic variation is a fundamental problem in genetics and evolutionary biology. The introduction of new genetic variation through mutation can lead to new adaptive phenotypes, but the complexity of the genotype-to-phenotype map makes it challenging to predict the phenotypic effects of ...
    Genome-wide Estimation of Evolutionary Distance and Phylogenetic Analysis of Homologous Genes
    进化距离的全基因组评估和同源基因的系统进化分析
    作者:Meixia Zhao, Biao Zhang, Jianxin Ma and Damon Lisch日期:12/05/2018,浏览量:3169,Q&A: 0
    [Abstract] Homologous genes, including paralogs and orthologs, are genes that share sequence homologies within or between different species. Homologous genes originate from a common origin through speciation, genetic duplication or horizontal gene transfer. Estimation of the sequence divergence of homologous genes help us to understand divergence time, which ...
    Bacterial Microcolonies in Gel Beads for High-throughput Screening
    凝胶珠细菌微菌落的高通量筛选
    作者:Yolanda Schaerli日期:07/05/2018,浏览量:4599,Q&A: 0
    [Abstract] High-throughput screening of a DNA library expressed in a bacterial population for identifying potentially rare members displaying a property of interest is a crucial step for success in many experiments such as directed evolution of proteins and synthetic circuits and deep mutational scanning to identify gain- or loss-of-function mutants.
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    Extraction and 16S rRNA Sequence Analysis of Microbiomes Associated with Rice Roots
    水稻根系相关微生物群落的分离及其16S rRNA序列分析
    作者:Joseph Edwards, Christian Santos-Medellín and Venkatesan Sundaresan日期:06/20/2018,浏览量:7993,Q&A: 0
    [Abstract] Plant roots associate with a wide diversity of bacteria and archaea across the root-soil spectrum. The rhizosphere microbiota, the communities of microbes in the soil adjacent to the root, can contain up to 10 billion bacterial cells per gram of soil (Raynaud and Nunan, 2014) and can play important roles for the fitness of the host plant. Subsets ...
    Single-step Precision Genome Editing in Yeast Using CRISPR-Cas9
    使用CRISPR-Cas9在酵母中进行单步精确基因组编辑
    [Abstract] Genome modification in budding yeast has been extremely successful largely due to its highly efficient homology-directed DNA repair machinery. Several methods for modifying the yeast genome have previously been described, many of them involving at least two-steps: insertion of a selectable marker and substitution of that marker for the intended ...
    Extraction of DNA from Murine Fecal Pellets for Downstream Phylogenetic Microbiota Analysis by Next-generation Sequencing
    采用新一代测序技术从小鼠粪粒中提取DNA进行下游微生物群进化分析
    作者:Elien Eeckhout and Andy Wullaert日期:02/05/2018,浏览量:6789,Q&A: 2
    [Abstract] Mouse models are widely used to evaluate the potential impact of the gut microbial composition on health and disease. Standardized protocols for sampling and storing murine feces, as well as for extracting DNA from these fecal pellets are needed to limit experimental variation between different studies. Both efficient lysis of the microbiota and ...
    Brief Protocol for EDGE Bioinformatics: Analyzing Microbial and Metagenomic NGS Data
    EDGE生物信息学工具简要使用方法:分析微生物和宏基因组学下一代测序数据
    [Abstract] Next-generation sequencing (NGS) offers unparalleled resolution for untargeted organism detection and characterization. However, the majority of NGS analysis programs require users to be proficient in programming and command-line interfaces. EDGE bioinformatics was developed to offer scientists with little to no bioinformatics expertise a ...
    Validating Candidate Congenital Heart Disease Genes in Drosophila
    果蝇中候选先天性心脏病基因的验证
    作者:Jun-yi Zhu, Yulong Fu, Adam Richman and Zhe Han日期:06/20/2017,浏览量:5422,Q&A: 0
    [Abstract] Genomic sequencing efforts can implicate large numbers of genes and de novo mutations as potential disease risk factors. A high throughput in vivo model system to validate candidate gene association with pathology is therefore useful. We present such a system employing Drosophila to validate candidate congenital heart ...