系统生物学

分类

    现刊
    Genome-wide Estimation of Evolutionary Distance and Phylogenetic Analysis of Homologous Genes
    进化距离的全基因组评估和同源基因的系统进化分析
    作者:Meixia Zhao, Biao Zhang, Jianxin Ma and Damon Lisch日期:12/05/2018,浏览量:1417,Q&A: 0
    [Abstract] Homologous genes, including paralogs and orthologs, are genes that share sequence homologies within or between different species. Homologous genes originate from a common origin through speciation, genetic duplication or horizontal gene transfer. Estimation of the sequence divergence of homologous genes help us to understand divergence time, which ...
    Bacterial Microcolonies in Gel Beads for High-throughput Screening
    凝胶珠细菌微菌落的高通量筛选
    作者:Yolanda Schaerli日期:07/05/2018,浏览量:2925,Q&A: 0
    [Abstract] High-throughput screening of a DNA library expressed in a bacterial population for identifying potentially rare members displaying a property of interest is a crucial step for success in many experiments such as directed evolution of proteins and synthetic circuits and deep mutational scanning to identify gain- or loss-of-function mutants.
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    Extraction and 16S rRNA Sequence Analysis of Microbiomes Associated with Rice Roots
    水稻根系相关微生物群落的分离及其16S rRNA序列分析
    作者:Joseph Edwards, Christian Santos-Medellín and Venkatesan Sundaresan日期:06/20/2018,浏览量:4557,Q&A: 0
    [Abstract] Plant roots associate with a wide diversity of bacteria and archaea across the root-soil spectrum. The rhizosphere microbiota, the communities of microbes in the soil adjacent to the root, can contain up to 10 billion bacterial cells per gram of soil (Raynaud and Nunan, 2014) and can play important roles for the fitness of the host plant. Subsets ...
    Single-step Precision Genome Editing in Yeast Using CRISPR-Cas9
    使用CRISPR-Cas9在酵母中进行单步精确基因组编辑
    [Abstract] Genome modification in budding yeast has been extremely successful largely due to its highly efficient homology-directed DNA repair machinery. Several methods for modifying the yeast genome have previously been described, many of them involving at least two-steps: insertion of a selectable marker and substitution of that marker for the intended ...
    Extraction of DNA from Murine Fecal Pellets for Downstream Phylogenetic Microbiota Analysis by Next-generation Sequencing
    采用新一代测序技术从小鼠粪粒中提取DNA进行下游微生物群进化分析
    作者:Elien Eeckhout and Andy Wullaert日期:02/05/2018,浏览量:4077,Q&A: 2
    [Abstract] Mouse models are widely used to evaluate the potential impact of the gut microbial composition on health and disease. Standardized protocols for sampling and storing murine feces, as well as for extracting DNA from these fecal pellets are needed to limit experimental variation between different studies. Both efficient lysis of the microbiota and ...
    Brief Protocol for EDGE Bioinformatics: Analyzing Microbial and Metagenomic NGS Data
    EDGE生物信息学工具简要使用方法:分析微生物和宏基因组学下一代测序数据
    [Abstract] Next-generation sequencing (NGS) offers unparalleled resolution for untargeted organism detection and characterization. However, the majority of NGS analysis programs require users to be proficient in programming and command-line interfaces. EDGE bioinformatics was developed to offer scientists with little to no bioinformatics expertise a ...
    Validating Candidate Congenital Heart Disease Genes in Drosophila
    果蝇中候选先天性心脏病基因的验证
    作者:Jun-yi Zhu, Yulong Fu, Adam Richman and Zhe Han日期:06/20/2017,浏览量:4195,Q&A: 0
    [Abstract] Genomic sequencing efforts can implicate large numbers of genes and de novo mutations as potential disease risk factors. A high throughput in vivo model system to validate candidate gene association with pathology is therefore useful. We present such a system employing Drosophila to validate candidate congenital heart ...
    A Method to Convert mRNA into a Guide RNA (gRNA) Library without Requiring Previous Bioinformatics Knowledge of the Organism
    不需预知生物体生物信息学条件下将其mRNA转化为向导RNA(gRNA)库的方法
    作者:Hiroshi Arakawa日期:05/20/2017,浏览量:4889,Q&A: 0
    [Abstract] While the diversity of species represents a diversity of special biological abilities, many of the genes that encode those special abilities in a variety of species are untouched, leaving an untapped gold mine of genetic information; however, despite current advances in genome bioinformatics, annotation of that genetic information is incomplete in ...
    Next-generation Sequencing of the DNA Virome from Fecal Samples
    粪便样本中DNA病毒组的下一代测序法
    作者:Cynthia L. Monaco and Douglas S. Kwon日期:03/05/2017,浏览量:5181,Q&A: 0
    [Abstract] Herein we describe a detailed protocol for DNA virome analysis of low input human stool samples (Monaco et al., 2016). This protocol is divided into four main steps: 1) stool samples are pulverized to evenly distribute microbial matter; 2) stool is enriched for virus-like particles and DNA is extracted by phenol-chloroform; 3) purified ...
    Sequencing of Ebola Virus Genomes Using Nanopore Technology
    采用纳米孔技术对埃博拉病毒测序
    作者:Thomas Hoenen日期:11/05/2016,浏览量:6035,Q&A: 0
    [Abstract] Sequencing of virus genomes during disease outbreaks can provide valuable information for diagnostics, epidemiology, and evaluation of potential countermeasures. However, particularly in remote areas logistical and technical challenges can be significant. Nanopore sequencing provides an alternative to classical Sanger and next-generation ...