细胞生物学

分类

    现刊
    Analysis of Generalized Fibrosis in Mouse Tissue Sections with Masson’s Trichrome Staining
    Masson Trichrome染色法分析小鼠组织切片中的普遍纤维化
    作者:Diantha van de Vlekkert, Eda Machado and Alessandra d’Azzo日期:05/20/2020,浏览量:705,Q&A: 0
    [Abstract] Expansion of fibrous connective tissue and abnormal deposition of extracellular matrix (ECM) are at the basis of many fibrotic diseases. Fibrosis can occur in response to both physiological and pathological cues, including wound healing, tissue remodeling/repair and inflammation. Chronic fibrosis can lead to severe tissue damage, organ failure and ...
    Application of Mechanical Forces on Drosophila Embryos by Manipulation of Microinjected Magnetic Particles
    机械力应用于果蝇胚胎的磁性粒子显微注射操作
    作者:Arturo D’Angelo and Jérôme Solon日期:05/05/2020,浏览量:620,Q&A: 0
    [Abstract] Cells generate mechanical forces to shape tissues during morphogenesis. These forces can activate several biochemical pathways and trigger diverse cellular responses by mechano-sensation, such as differentiation, division, migration and apoptosis. Assessing the mechano-responses of cells in living organisms requires tools to apply controlled local ...
    Method for Measuring Mucociliary Clearance and Cilia-generated Flow in Mice by ex vivo Imaging
    小鼠中黏膜纤毛清除和纤毛产生流动的离体成像检测方法
    作者:Eric Song and Akiko Iwasaki日期:03/20/2020,浏览量:644,Q&A: 0
    [Abstract] Ex vivo biophysical measurements provide valuable insights into understanding both physiological and pathogenic processes. One critical physiological mechanism that is regulated by these biophysical properties is cilia-generated flow that mediates mucociliary clearance, which is known to provide protection against foreign particles and ...
    Testing Bone Formation Induction by Calvarial Injection Assay in vivo
    体内颅骨注射分析检测骨形成
    作者:Narelle E McGregor, Ingrid J Poulton, Emma C Walker and Natalie A Sims日期:03/20/2020,浏览量:831,Q&A: 0
    [Abstract] Bone formation occurs during embryogenesis, skeletal growth and during the process of skeletal renewal throughout life. In the process of bone formation, osteoblasts lay down a collagen-containing matrix, termed osteoid, which is gradually hardened by incorporation of mineral crystals. Although osteoblasts can be induced to differentiate and to ...
    Preparation of Single Epithelial Cells Suspension from Mouse Mammary Glands
    小鼠乳腺单个上皮细胞悬液制备
    作者:Heng Sun, Xiaoling Xu and Chuxia Deng日期:02/20/2020,浏览量:1014,Q&A: 0
    [Abstract] Single cell RNA sequencing is a very powerful means for cellular heterogeneous studies and so becoming wildly utilized nowadays. To guarantee the success of such analysis, it is very important, though sometimes difficult, to obtain single cells suspension with high quality, especially from the primary solid organs like mammary glands. Digestion of ...
    Bone-in-culture Array to Model Bone Metastasis in ex vivo Condition
    体外骨碎片培养列阵模拟骨转移
    作者:Hai Wang and Xiang H.-F. Zhang日期:01/20/2020,浏览量:888,Q&A: 0
    [Abstract] Bone is the most frequently affected organ by metastases of breast cancer and prostate cancer. Our knowledge on bone metastasis is extremely limited due to the lack of potent and efficient experimental models. We developed the “Bone-In-Culture Array (BICA)” platform to model the bone colonization of cancer cells in ex vivo cultures. The ...
    Characterization of Biological Motion Using Motion Sensing Superpixels
    基于运动传感超像素的生物运动特性研究
    作者:Felix Y. Zhou, Carlos Ruiz-Puig, Richard P. Owen, Michael J. White, Jens Rittscher and Xin Lu日期:09/20/2019,浏览量:1519,Q&A: 0
    [Abstract] Precise spatiotemporal regulation is the foundation for the healthy development and maintenance of living organisms. All cells must correctly execute their function in the right place at the right time. Cellular motion is thus an important dynamic readout of signaling in key disease-relevant molecular pathways. However despite the rapid ...
    A Novel Technique for Imaging and Analysis of Hair Cells in the Organ of Corti Using Modified Sca/eS and Machine Learning
    利用改进的Sca/eS和机器学习用于螺旋器中毛细胞的成像和分析
    [Abstract] Here, we describe a sorbitol-based optical clearing method, called modified Sca/eS that can be used to image all hair cells (HCs) in the mouse cochlea. This modification of Sca/eS is defined by three steps: decalcification, de-lipidation, and refractive index matching, which can all be completed within 72 h. Furthermore, we established automated ...
    Looking through Brains with Fast Passive CLARITY: Zebrafish, Rodents, Non-human Primates and Humans
    利用 Fast Passive CLARITY技术研究斑马鱼、啮齿动物、灵长类动物和人类的大脑
    作者:Farzad Mortazavi, Alexander J. Stankiewicz and Irina V. Zhdanova日期:08/05/2019,浏览量:2879,Q&A: 0
    [Abstract] Recently developed CLARITY (Clear Lipid-exchanged Acrylamide-hybridized Rigid Imaging/Immunostaining/In situ-hybridization-compatible Tis-sue-hYdrogel) technique renders the tissue transparent by removing lipids in the tissue, while preserving and stabilizing the cellular and subcellular structures. This provides effective penetration of diverse ...
    Precision Technique for Splenectomy Limits Mouse Stress Responses for Accurate and Realistic Measurements for Investigating Inflammation and Immunity
    脾切除术限制小鼠应激反应的精准技术用于精确现实检测炎症和免疫
    作者:Shengwen Calvin Li, Anthony D. Rangel and Mustafa H. Kabeer日期:08/05/2019,浏览量:1762,Q&A: 0
    [Abstract] Splenectomy in an animal model requires a standardized technique utilizing best practice to avoid variability which can result in adverse impact to the animal resulting in flawed physiologic responses simply due to technique rather than to the studied variables. In the case of the spleen, often investigators are analyzing the animal immune or ...