植物科学

分类

    现刊
    Quantification of Starch in Guard Cells of Arabidopsis thaliana
    定量测定拟南芥保卫细胞中的淀粉
    作者:Sabrina Flütsch, Luca Distefano and Diana Santelia日期:07/05/2018,浏览量:4989,Q&A: 0
    [Abstract] In this protocol, we describe how to quantify starch in guard cells of Arabidopsis thaliana using the fluorophore propidium iodide and confocal laser scanning microscopy. This simple method enables monitoring, with unprecedented resolution, the dynamics of starch in guard cells.
    Isolation of Intact Vacuoles from Petunia Petals and Extraction of Sequestered Glycosylated Phenylpropanoid Compounds
    从矮牵牛花瓣中分离完整的液泡并提取分离糖基化苯丙素类化合物
    作者:Oded Skaliter , Jasmin Ravid, Alon Cna'ani, Gony Dvir, Rafael Knafo and Alexander Vainstein日期:07/05/2018,浏览量:3860,Q&A: 0
    [Abstract] Plant vacuoles are the largest compartment in plant cells, occupying more than 80% of the cell volume. A variety of proteins, sugars, pigments and other metabolites are stored in these organelles (Paris et al., 1996; Olbrich et al., 2007). Flowers produce a variety of specialized metabolites, some of which are unique to this ...
    Radioactive Tracer Feeding Experiments and Product Analysis to Determine the Biosynthetic Capability of Comfrey (Symphytum officinale) Leaves for Pyrrolizidine Alkaloids
    放射性示踪剂喂养实验测定紫草(聚合草)叶的吡咯里西啶生物碱生物合成能力及其合成产物的分析
    作者:Thomas Stegemann, Lars H. Kruse and Dietrich Ober日期:02/05/2018,浏览量:3759,Q&A: 0
    [Abstract] This protocol delivers a method to determine the biosynthetic capability of comfrey leaves for pyrrolizidine alkaloids independently from other organs like roots or flowers.

    The protocol applies and combines radioactive tracer experiments with standard and modern techniques like thin layer chromatography (TLC), solid-phase extraction ...
    1-MCP (1-methylcyclopropene) Treatment Protocol for Fruit or Vegetables
    水果或蔬菜的1-MCP(1-甲基环丙烯)处理实验方案
    作者:Gamrasni Dan, Goldway Martin, Stern Yosi, Breitel Dario and Aharoni Asaph日期:05/20/2017,浏览量:11311,Q&A: 4
    [Abstract] 1-MCP (1-methylcyclopropene) is a simple synthetic hydrocarbon molecule that interacts with the ethylene receptor and inhibits the response of fruit or plant to ethylene. 1-MCP has opened new opportunities in handling harvested crops and serves as a powerful tool to learn about plant response to ethylene (Watkins and Miller, 2006). 1-MCP is ...
    Simple Spectroscopic Determination of Nitrate, Nitrite, and Ammonium in Arabidopsis thaliana
    拟南芥中硝酸盐、亚硝酸盐和铵的简单光谱测定
    作者:Takushi Hachiya and Yuki Okamoto日期:05/20/2017,浏览量:12448,Q&A: 1
    [Abstract] Plants use nitrate, nitrite, and ammonium as inorganic nitrogen (N) sources. These N compounds are included in plant tissues at various concentrations depending on the balance between their uptake and assimilation. Thus, the contents of nitrate, nitrite, and ammonium are physiological indicators of plant N economy. Here, we describe a protocol for ...
    Extraction and Analysis of Carotenoids from Escherichia coli in Color Complementation Assays
    在颜色互补分析实验中提取及分析大肠埃希杆菌中的类胡萝卜素
    作者:Andreas Blatt and Martin Lohr日期:03/20/2017,浏览量:8495,Q&A: 0
    [Abstract] A common method to investigate the function of genes putatively involved in carotenoid biosynthesis is the so called color complementation assay in Escherichia coli (see, e.g., Cunningham and Gantt, 2007). In this assay, the gene under investigation is expressed in E. coli strains genetically engineered to synthesize ...
    Acetyl Bromide Soluble Lignin (ABSL) Assay for Total Lignin Quantification from Plant Biomass
    采用乙酰溴可溶性木质素(ABSL)测定法定量植物生物量中的总木质素
    作者:William J. Barnes and Charles T. Anderson日期:03/05/2017,浏览量:10628,Q&A: 0
    [Abstract] Lignin is the second most abundant biopolymer on Earth, providing plants with mechanical support in secondary cell walls and defense against abiotic and biotic stresses. However, lignin also acts as a barrier to biomass saccharification for biofuel generation (Carroll and Somerville, 2009; Zhao and Dixon, 2011; Wang et al., 2013). For ...
    Rice Root Organic Acid Efflux Measurement by Using Ion Chromatography
    采用离子色谱法测定水稻根有机酸分泌
    作者:Chun-quan Zhu, Xiao-fang Zhu and Ren-fang Shen日期:02/20/2017,浏览量:5843,Q&A: 0
    [Abstract] Organic acids secreted from plant roots play important roles in various biological processes including nutrient acquisition, metal detoxification, and pathogen attraction. The secretion of organic acids may be affected by various conditions such as plant growth stage, nutrient deficiency, and abiotic stress. For example, when white lupin (Lupinus ...
    Metabolite Profiling of Mature Arabidopsis thaliana Seeds Using Gas Chromatography-Mass Spectrometry (GC-MS)
    采用气相色谱质谱仪(GC-MS)分析成熟拟南芥种子代谢谱
    作者:Hagai Cohen, Ifat Matityahu and Rachel Amir日期:11/05/2016,浏览量:7893,Q&A: 0
    [Abstract] Metabolite profiling using gas chromatography-mass spectrometry (GC-MS) permits the annotation and quantification of a relatively wide variety of metabolites, covering a wide range of biochemical groups of metabolites. Lisec et al. (2006) established a method for GC-MS profiling in plants. Based on this protocol, we provide here a ...
    Hydrogen Peroxide Measurement in Arabidopsis Root Tissue Using Amplex Red
    Amplex Red荧光法测定拟南芥根组织中的过氧化氢酶
    作者:Tzvetina Brumbarova, Cham Thi Tuyet Le and Petra Bauer日期:11/05/2016,浏览量:10639,Q&A: 0
    [Abstract] This protocol describes the measurement of hydrogen peroxide (H2O2) content in Arabidopsis root tissue by using the Amplex® Red Hydrogen Peroxide/Peroxidase Assay Kit. When root tissue is disrupted and resuspended in phosphate buffer, H2O2 is released from the cells. The obtained root ...