植物科学

分类

    现刊
    Production and Bioassay of a Diffusible Factor that Induces Gametophyte-to-Sporophyte Developmental Reprogramming in the Brown Alga Ectocarpus
    水云属褐藻配子体-孢子体发育重编程扩散因子的产生及生物测定
    [Abstract] The brown alga Ectocarpus has a haploid-diploid life cycle that involves alternation between two multicellular generations, the sporophyte and the gametophyte. Life cycle generation is not determined by ploidy but by a genetic system that includes two different three amino acid loop extension homeodomain transcription factors called ...
    PEG-mediated, Stable, Nuclear and Chloroplast Transformation of Cyanidioschizon merolae
    PEG介导的稳定的Cyanidioschizon merolae细胞核和叶绿体转化
    作者:Maksymilian Zienkiewicz, Tomasz Krupnik, Anna Drożak and Kinga Kania日期:09/05/2019,浏览量:1451,Q&A: 0
    [Abstract] The ability to achieve nuclear or chloroplast transformation in plants has been a long standing goal, especially in microalgae research. Over past years there has been only little success, but transient and stable nuclear transformation has been achieved in multiple species. Our newly developed method allows for relatively simple transformation of ...
    Multiple Modification of Chromosomal Loci Using URA5.3 Selection Marker in the Unicellular Red Alga Cyanidioschyzon merolae
    在单细胞红藻 Cyanidioschyzon merolae中利用URA5.3选择标记对染色体基因进行多重修饰
    作者:Tokiaki Takemura, Sousuke Imamura, Yuki Kobayashi and Kan Tanaka日期:04/05/2019,浏览量:2522,Q&A: 0
    [Abstract] The unicellular red alga Cyanidioschyzon merolae has been used as a eukaryotic photosynthetic model for various basic and applied studies. Although the nuclear genome of C. merolae can be modified by homologous recombination with exogenously introduced DNA, it has been difficult to modify multiple chromosome loci within the same ...
    Top Starch Plating Method for the Efficient Cultivation of Unicellular Red Alga Cyanidioschyzon merolae
    高效培养单细胞红藻花青素的顶部淀粉平板法
    作者:Tokiaki Takemura, Yuki Kobayashi, Sousuke Imamura and Kan Tanaka日期:02/20/2019,浏览量:3084,Q&A: 0
    [Abstract] The unicellular red alga Cyanidioschyzon merolae has been used as a model photosynthetic eukaryote for various basic and applied studies, and several of these molecular genetics techniques have been reported. However, there are still improvements to be made concerning the plating method. The conventional plating method often generates ...
    Determination of Storage (Starch/Glycogen) and Total Saccharides Content in Algae and Cyanobacteria by a Phenol-Sulfuric Acid Method
    苯酚-硫酸法测定藻类和蓝藻中淀粉/糖原的储存和总糖含量
    作者:Tomáš Zavřel, Petra Očenášová, Maria A. Sinetova and Jan Červený日期:08/05/2018,浏览量:4956,Q&A: 0
    [Abstract] This is a protocol for quantitative determination of storage and total carbohydrates in algae and cyanobacteria. The protocol is simple, fast and sensitive and it requires only few standard chemicals. Great advantage of this protocol is that both storage and total saccharides can be determined in the cellular pellets that were already used for ...
    Genome Editing in Diatoms Using CRISPR-Cas to Induce Precise Bi-allelic Deletions
    通过CRISPR-Cas精确诱导双等位基因缺失以对硅藻进行基因组编辑
    作者:Amanda Hopes, Vladimir Nekrasov, Nigel Belshaw, Irina Grouneva, Sophien Kamoun and Thomas Mock日期:12/05/2017,浏览量:7816,Q&A: 1
    [Abstract] Genome editing in diatoms has recently been established for the model species Phaeodactylum tricornutum and Thalassiosira pseudonana. The present protocol, although developed for T. pseudonana, can be modified to edit any diatom genome as we utilize the flexible, modular Golden Gate cloning system. The main steps include ...
    ROS Detection in Botryococcus braunii Colonies with CellROX Green Reagent
    使用CellROX绿色试剂检测布朗葡萄藻集落中的ROS
    作者:Edmundo Lozoya-Gloria, Ivette Cornejo-Corona, Hem R. Thapa, Daniel R. Browne and Timothy P. Devarenne日期:08/20/2017,浏览量:5286,Q&A: 0
    [Abstract] We analyzed the reactive oxygen species (ROS) accumulation in the colony-forming green microalga Botryococcus braunii in response to several stress inducers such as NaCl, NaHCO3, salicylic acid (SA), methyl jasmonate, and acetic acid. A staining assay using the fluorescent dye CellROX Green was used. CellROX Green is a ...
    Determination of Reduced and Total Glutathione Content in Extremophilic Microalga Galdieria phlegrea
    极端环境微藻Galdieria phlegrea中还原型和总谷胱甘肽含量的测定
    作者:Giovanna Salbitani, Claudia Bottone and Simona Carfagna日期:07/05/2017,浏览量:12900,Q&A: 2
    [Abstract] Glutathione is an important molecule involved in the primary and secondary metabolism of all organisms. The Glutathione redox status is an indicator of the cellular redox state. Therefore, it is important to have precise methods on hand to determine the glutathione redox status in the cell. In this protocol, we describe an improved ...
    Cryopreservation Protocol for Chlamydomonas reinhardtii
    莱茵衣藻的冷冻保存方案
    作者:Duanpeng Yang and Weiqi Li日期:11/20/2016,浏览量:5937,Q&A: 0
    [Abstract] Cryopreservation is commonly used for storing viable cells, tissues, organs or organisms at ultralow temperatures, and usually involves immersion in liquid nitrogen at -196 °C. Here we provide a detailed cryopreservation protocol for C. reinhardtii based on Crutchfield’s work (Crutchfield et al., 1999), with minor changes (Yang ...
    Determination of Recombinant Mannitol-1-phosphate Dehydrogenase Activity from Ectocarpus sp.
    水云属中重组甘露醇-1-磷酸脱氢酶活性的测定
    作者:Agnès Groisillier and Thierry Tonon日期:11/05/2016,浏览量:5668,Q&A: 0
    [Abstract] Brown algae belong to a phylogenetic lineage distantly related to green plants and animals, and are found predominantly, but not exclusively, in the intertidal zone, a harsh and frequently changing environment. Because of their unique evolutionary history and of their habitat, brown algae feature several peculiarities in their metabolism. One of ...