分子生物学

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    现刊
    Paper Lateral Flow Biosensor for Nodavirus Reverse Transcribed RNA Detection
    用于诺达病毒逆转录RNA检测的纸侧向流生物传感器
    作者:Dimitra K. Toubanaki and Evdokia Karagouni日期:08/05/2020,浏览量:169,Q&A: 0
    [Abstract] Paper nanobiosensors have been established as an excellent platform for analysis of veterinary and human pathogens causing various diseases. Especially, lateral flow assays or biosensors ideal for sensitive, rapid, robust and accurate analysis in laboratory setups and on-site analysis. Viral RNA detection is of great importance for public health ...
    Preparation of Yeast tRNA Sample for NMR Spectroscopy
    酵母tRNA样品的核磁共振制备
    作者:Marjorie Catala, Alexandre Gato, Carine Tisné and Pierre Barraud日期:06/20/2020,浏览量:639,Q&A: 0
    [Abstract] Transfer RNAs (tRNAs) are heavily decorated with post-transcriptional modifications during their biosynthesis. To fulfil their functions within cells, tRNAs undergo a tightly controlled biogenesis process leading to the formation of mature tRNAs. In addition, functions of tRNAs are often modulated by their modifications. Although the biological ...
    Low-cost and Multiplexable Whole mRNA-Seq Library Preparation Method with Oligo-dT Magnetic Beads for Illumina Sequencing Platforms
    Oligo-dT磁珠用于Illumina测序平台的低成本和可复用的完整mRNA-Seq文库制备方法
    作者:Makoto Kashima, Ayumi Deguchi, Ayumi Tezuka and Atsushi J. Nagano日期:06/20/2020,浏览量:1107,Q&A: 0
    [Abstract] RNA-Seq is a powerful method for transcriptome analysis used in varied field of biology. Although several commercial products and hand-made protocols enable us to prepare RNA-Seq library from total RNA, their cost are still expensive. Here, we established a low-cost and multiplexable whole mRNA-Seq library preparation method for illumine ...
    Biochemical Pulldown of mRNAs and Long Noncoding RNAs from Cellular Lysates Coupled with Mass Spectrometry to Identify Protein Binding Partners
    通过细胞裂解液中mRNAs和长链非编码RNA的生化沉降结合质谱法鉴定蛋白结合物
    作者:Anca F. Savulescu, Stoyan Stoychev, Sipho Mamputha and Musa M. Mhlanga日期:06/05/2020,浏览量:978,Q&A: 0
    [Abstract] RNA binding proteins (RBPs) interact with cellular mRNAs, controlling various steps throughout the lifetime of these transcripts, including transcription, cellular transport, subcellular localization, translation and degradation. In addition to binding mRNA transcripts, a growing number of RBPs are shown to bind long noncoding RNAs (lncRNAs), ...
    RNA Extraction from Ears and Draining Lymph Nodes of Mice Infected with Leishmania amazonensis
    亚马逊利什曼原虫感染小鼠耳及引流淋巴结RNA的提取
    作者:Emilie Giraud and Evie Melanitou日期:06/05/2020,浏览量:642,Q&A: 0
    [Abstract] Parasites of the genus Leishmania infect the mammalian hosts, including mice and humans and cause cutaneous or visceral leishmaniasis depending upon the parasite species transmitted by the vector sandfly. Leishmania amazonensis is one of the Leishmania species responsible for the cutaneous form of the disease. We have ...
    Confocal and Super-resolution Imaging of RNA in Live Bacteria Using a Fluorogenic Silicon Rhodamine-binding Aptamer
    含氟硅-罗丹明适配体用于活性菌中RNA的激光共聚焦超分辨率成像
    作者:Regina Wirth, Peng Gao, G. Ulrich Nienhaus, Murat Sunbul and Andres Jäschke日期:05/05/2020,浏览量:1391,Q&A: 0
    [Abstract] Genetically encoded light-up RNA aptamers have been shown to be promising tools for the visualization of RNAs in living cells, helping us to advance our understanding of the broad and complex life of RNA. Although a handful of light-up aptamers spanning the visible wavelength region have been developed, none of them have yet been reported to be ...
    Viral Double-Stranded RNA Detection by DNase I and Nuclease S1 digestions in Leishmania parasites
    在利什曼原虫中利用DNase I和 Nuclease S1酶解进行病毒双链RNA鉴定
    作者:Nathalie Isorce and Nicolas Fasel日期:05/05/2020,浏览量:606,Q&A: 0
    [Abstract] Many RNA viruses are found in protozoan parasites. They can be responsible for more serious pathology or treatment failure. For the detection of viral double-stranded RNA (dsRNA), sequence-dependent and -independent methods are available, such as quantitative real-time PCR and immunofluorescence, dot blot, ELISA or sequencing. The technique ...
    High-throughput Flow Cytometry Assay to Investigate TDP43 Splicing Function
    高通量流式细胞术检测TDP43剪接功能
    作者:H. Broder Schmidt and Rajat Rohatgi日期:04/20/2020,浏览量:1190,Q&A: 0
    [Abstract] Mutations in RNA-binding proteins (RBPs) such as TDP43 are associated with transcriptome-wide splicing defects and cause severe neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). The impact of RBP mutations on splicing function is routinely studied using PCR-based bulk measurements. ...
    Circadian Gene Profiling in Laser Capture Microdissected Mouse Club Cells
    激光捕获显微解剖小鼠棒状细胞的昼夜节律基因分析
    作者:Zhenguang Zhang and Andrew Loudon日期:04/20/2020,浏览量:870,Q&A: 0
    [Abstract] Cell heterogeneity is high in tissues like lung. Research conducted on pure population of cells usually offers more insights than bulk tissues, such as circadian clock work. In this protocol, we provide a detailed work flow on how to do circadian clock study by RNA seq in laser capture micro-dissected mouse lung club cells. The method uses frozen ...
    Real-time Fluorescence Measurement of Enterovirus Uncoating
    肠道病毒脱壳的实时荧光检测
    作者:Visa Ruokolainen, Mira Laajala and Varpu Marjomäki日期:04/05/2020,浏览量:769,Q&A: 0
    [Abstract] Viruses need to open, i.e., uncoat, in order to release their genomes for efficient replication and translation. Especially for non-enveloped viruses, such as enteroviruses, the cues leading to uncoating are less well known. The status of the virus has previously been observed mainly by transmission electron microscopy using negative ...