分子生物学

分类

    现刊
    Construction of Viral Vectors for Cell Type-specific CRISPR Gene Editing in the Adult Mouse Brain
    用于成年小鼠脑组织的细胞特异性CRISPR基因编辑的病毒载体的构建
    作者:Hiroshi Yamaguchi and Luis de Lecea日期:08/20/2019,浏览量:6,Q&A: 0
    [Abstract] Recently developed gene editing technologies based on engineered CRISPR/Cas9 systems enables researchers to disrupt genes in a cell type-specific manner in the adult mouse brain. Using these technologies, we recently showed that the dopamine beta-hydroxylase gene in Locus Coeruleus (LC) norepinephrine neurons plays a vital role in the maintenance ...
    Measurement of the Length of the Integrated Donor DNA during Bacillus subtilis Natural Chromosomal Transformation
    枯草芽孢杆菌自然遗传转化过程中完整供体DNA长度的测定
    作者:Ester Serrano and Begoña Carrasco日期:08/20/2019,浏览量:6,Q&A: 0
    [Abstract] For natural transformation to occur, bacterial cells must first develop a programmed physiological state called competence. Competence in Bacillus subtilis, which occurs only in a fraction of cells, is a transient stress response that allows cells to take up DNA from the environment. During natural chromosomal transformation, the ...
    Genotyping of the OATP1B1 c. 521 T>C Polymorphism from the Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Specimens: An Optimized Protocol
    对FFPE组织样品中OATP1B1 c. 521 T>C多态性进行基因分型的优化实验方法
    作者:Alexandra R Crowe, Jonathan Miller and Wei Yue日期:08/20/2019,浏览量:7,Q&A: 0
    [Abstract] Organic anion transporting polypeptide (OATP) 1B1 is a liver-specific transport protein that plays an important role in hepatic drug disposition. It transports many drugs from the blood into the liver, including lipid-lowering statins. The c. 521 T>C polymorphism of OATP1B1 has reduced transport activity and is associated with statin-induced ...
    Solid Phase PCR on 3D Microstructure ArrayChip for Pathogen Detection Application
    用于病原体检测的三维显微组织列阵中固相PCR
    作者:Krishna Kant and Tien Anh Ngo日期:08/05/2019,浏览量:196,Q&A: 0
    [Abstract] Advanced free angle photolithography (FAPL) is presented for making 3D supercritical angle fluorescence (SAF) microstructures and transfer them on to polymeric chips using injection molding technique for low-cost microfluidic devices embedded with optical sensing structures. A solid phase polymerase chain reaction (SP-PCR) is used as model ...
    Strand-specific Single-stranded DNA Sequencing (4S-seq) of E. coli genomes
    大肠杆菌特异性单链DNA测序(4S-seq)
    作者:Takahiro Masuda, Nobuaki Kono, Masaru Tomita and Kazuharu Arakawa日期:08/05/2019,浏览量:205,Q&A: 0
    [Abstract] Most bacterial genomes have biased nucleotide composition, and the asymmetry is considered to be caused by a single-stranded DNA (ssDNA) deamination arising from the bacterial replication machinery. In order to evaluate the relationship experimentally, the position and frequency of ssDNA formed during replication must be verified clearly. Although ...
    Ex vivo Analysis of DNA Repair Capacity of Human Peripheral Blood Mononuclear Cells by a Modified Host Cell Reactivation Assay
    利用改进的宿主细胞再活化反应进行人外周血单核细胞的DNA修复能力的离体检测
    作者:Katja Matt and Jörg Bergemann日期:08/05/2019,浏览量:139,Q&A: 0
    [Abstract] The ability of humans to repair DNA damages decreases with increasing age. In order to be able to repair daily occurring DNA damages, it becomes more and more important to preserve repair capability of cells with aging. The preservation of DNA repair processes contributes to preventing DNA mutations and subsequently the onset of age-related ...
    Detection of Heteroplasmic Variants in the Mitochondrial Genome through Massive Parallel Sequencing
    大规模平行测序检测线粒体基因组异质粒
    作者:Joke Mertens, Filippo Zambelli, Dorien Daneels, Ben Caljon, Karen Sermon and Claudia Spits日期:07/05/2019,浏览量:452,Q&A: 0
    [Abstract] Detecting heteroplasmies in the mitochondrial DNA (mtDNA) has been a challenge for many years. In the past, Sanger sequencing was the main option to perform this analysis, however, this method could not detect low frequency heteroplasmies. Massive Parallel Sequencing (MPS) provides the opportunity to study the mtDNA in depth, but a controlled ...
    A High-throughput qPCR-based Method to Genotype the SOD1G93A Mouse Model for Relative Copy Number
    一种基于qPCR 的高通量研究 SOD1G93A小鼠模型中相对拷贝数的基因分型方法
    作者:Valerie R. Tassinari and Fernando G. Vieira日期:06/20/2019,浏览量:706,Q&A: 0
    [Abstract] The most commonly used mouse model in ALS preclinical research expresses multiple copies of the human SOD1 (G93A) transgene. During the course of breeding, successive generations of mice can lose copies of the transgene. Because shorter lifespan of these mice is dependent on transgene copy number, it is essential to ensure that no low-copy, and ...
    Minigene Assay to Evaluate CRISPR/Cas9-based Excision of Intronic Mutations that Cause Aberrant Splicing in Human Cells
    Minigene技术评估会导致人细胞产生异常剪切的基于CRISPR/Cas9 的内含子突变切除
    作者:David J. Sanz and Patrick T. Harrison日期:06/05/2019,浏览量:480,Q&A: 0
    [Abstract] The construction of Hybrid minigenes provides a robust and simple strategy to study the effects of disease-causing mutations on mRNA splicing when biological material from patient cells is not available. Hybrid minigenes can be used as splicing reporter plasmids allow RNA expression and heterologous splicing reactions between synthetic splicing ...
    Improved HTGTS for CRISPR/Cas9 Off-target Detection
    改进的HTGTS用于 CRISPR/Cas9脱靶检测
    作者:Jianhang Yin, Mengzhu Liu, Yang Liu and Jiazhi Hu日期:05/05/2019,浏览量:725,Q&A: 0
    [Abstract] Precise genome editing is essential for scientific research and clinical application. At present, linear amplification-mediated high-throughput genome-wide translocation sequencing (LAM-HTGTS) is one of most effective methods to evaluate the off-target activity of CRISPR-Cas9, which is based on chromosomal translocation and employs a “bait” DNA ...