分子生物学

分类

    现刊
    Genomic Edition of Ashbya gossypii Using One-vector CRISPR/Cas9
    利用一个CRISPR/Cas9载体对棉囊阿舒氏酵母进行基因组编辑
    作者:Gloria Muñoz-Fernández, Alberto Jiménez and José Luis Revuelta日期:06/20/2020,浏览量:371,Q&A: 0
    [Abstract] The CRISPR/Cas9 system is a novel genetic tool which allows the precise manipulation of virtually any genomic sequence. In this protocol, we use a specific CRISPR/Cas9 system for the manipulation of Ashbya gossypii. The filamentous fungus A. gossypii is currently used for the industrial production of riboflavin (vitamina B2). In ...
    Safe DNA-extraction Protocol Suitable for Studying Tree-fungus Interactions
    适于研究树-真菌互作的安全DNA提取方法
    作者:Susanna Keriö, Eeva Terhonen and Jared M. LeBoldus日期:06/05/2020,浏览量:953,Q&A: 0
    [Abstract] We present a safe and low-cost method suitable for DNA extraction from mycelium and tree tissue samples. After sample preparation, the extraction takes about 60 min. Method performance was tested by extracting DNA from various tree tissue samples and from mycelium grown on solid and liquid media. DNA was extracted from juvenile and mature host ...
    Modeling NOTCH1 driven T-cell Acute Lymphoblastic Leukemia in Mice
    NOTCH1诱导小鼠T细胞急性淋巴细胞白血病模型的建立
    作者:Agnieszka A. Wendorff and Adolfo A. Ferrando日期:05/20/2020,浏览量:645,Q&A: 0
    [Abstract] T-cell acute lymphoblastic leukemia (T-ALL) is an aggressive hematological malignancy that arises from transformation of T-cell primed hematopoietic progenitors. Although T-ALL is a heterogenous and molecularly complex disease, more than 65% of T-ALL patients carry activating mutations in the NOTCH1 gene. The majority of T-ALL–associated ...
    Evaluation of the Efficiency of Genome Editing Tools by a Frameshift Fluorescence Protein Reporter
    通过移码荧光蛋白报告基因评估基因组编辑工具的效率
    作者:Balaji T. Moorthy, Akhilesh Kumar, Lauren X. Lotenfoe and Fangliang Zhang日期:05/20/2020,浏览量:757,Q&A: 0
    [Abstract] In the last decade, genome editing has been the center of attention as a novel tool for mechanistic investigations and for potential clinical applications. Various genome editing tools like meganucleases, zinc finger nucleases (ZFNs), transcription activator-like effector-based nucleases (TALEN), and the clustered regularly interspaced short ...
    Preparation of a Bacteriophage T4-based Prokaryotic-eukaryotic Hybrid Viral Vector for Delivery of Large Cargos of Genes and Proteins into Human Cells
    用于将基因和蛋白质转运至人体细胞中的基于噬菌体T4的大容量原核真核杂交病毒载体的制备
    作者:Jingen Zhu, Pan Tao, Marthandan Mahalingam and Venigalla B. Rao日期:04/05/2020,浏览量:482,Q&A: 0
    [Abstract] A viral vector that can safely and efficiently deliver large and diverse molecular cargos into cells is the holy grail of curing many human diseases. Adeno-associated virus (AAV) has been extensively used but has a very small capacity. The prokaryotic virus T4 has a large capacity but lacks natural mechanisms to enter mammalian cells. Here, we ...
    Conjugation Protocol Optimised for Roseburia inulinivorans and Eubacterium rectale
    食葡糖罗斯拜瑞氏菌和直肠真杆菌结合的优化方案
    作者:Paul O. Sheridan, Jennifer C. Martin and Karen P. Scott日期:04/05/2020,浏览量:449,Q&A: 0
    [Abstract] Roseburia and Eubacterium species of the human gut microbiota play an important role in the maintaince of human health, partly by producing butyrate, the main energy source of our colonic epithelial cells. However, our knowledge of the biochemistry and physiology of these bacteria has been limited by a lack of genetic ...
    Assembly of Genetic Circuits with the Mammalian ToolKit
    用哺乳动物工具包组装遗传电路
    作者:João P. Fonseca, Alain R Bonny, Jason Town and Hana El-Samad日期:03/05/2020,浏览量:892,Q&A: 0
    [Abstract] The ability to rapidly assemble and prototype cellular circuits is vital for biological research and its applications in biotechnology and medicine. The Mammalian ToolKit (MTK) is a Golden Gate-based cloning toolkit for fast, reproducible and versatile assembly of large DNA vectors and their implementation in mammalian models. The MTK consists of ...
    Construction of Antisense RNA-mediated Gene Knock-down Strains in the Cyanobacterium Anabaena sp. PCC 7120
    反义RNA介导的鱼腥藻PCC 7120基因敲除株的构建
    作者:Amit Srivastava, Anand Ballal, Karl Forchhammer and Anil Kumar Tripathi日期:02/20/2020,浏览量:933,Q&A: 0
    [Abstract] Anabaena sp. PCC 7120 (hereafter Anabaena) is a model cyanobacterium to study nitrogen fixation, cellular differentiation and several other key biological functions that are analogous in plants. As with any other organism, many genes in Anabaena encode an essential life function and hence cannot be deleted, causing a ...
    ChIP-Seq from Limited Starting Material of K562 Cells and Drosophila Neuroblasts Using Tagmentation Assisted Fragmentation Approach
    用Tagmentation Assisted Fragmentation法从K562细胞和果蝇神经母细胞的有限起始材料中提取芯片序列
    作者:Junaid Akhtar, Piyush More and Steffen Albrecht日期:02/20/2020,浏览量:630,Q&A: 0
    [Abstract] Chromatin immunoprecipitation is extensively used to investigate the epigenetic profile and transcription factor binding sites in the genome. However, when the starting material is limited, the conventional ChIP-Seq approach cannot be implemented. This protocol describes a method that can be used to generate the chromatin profiles from as low as ...
    Transcervical Mouse Infections with Chlamydia trachomatis and Determination of Bacterial Burden
    小鼠宫颈沙眼衣原体感染及细菌负荷测定
    作者:Karthika Rajeeve and Rajeeve Sivadasan日期:02/05/2020,浏览量:1045,Q&A: 0
    [Abstract] Chlamydia trachomatis is an obligate human pathogen. It infects the genital tract of humans ascending into the fallopian tube, exacerbated by chronic pelvic pain, pelvic inflammatory disease, and fallopian tube scaring resulting in infertility and other malignancies. The major hurdle in controlling chlamydial spread is that the ...