植物科学

分类

    现刊
    Acidified Blue Ink-staining Procedure for the Observation of Fungal Structures Inside Roots of Two Disparate Plant Lineages
    两个不同植物谱系根内真菌结构的酸化蓝墨水染色法观察流程
    作者:Jill Kowal, Elena Arrigoni and Sophie Lane日期:10/20/2020,浏览量:189,Q&A: 0
    Identifying microscopic mycorrhizal fungal structures in roots, i.e., hyphae, vesicles and arbuscules, requires root staining procedures that are often time consuming and involves chemicals known to present health risks from exposure. By ...
    Low-cost and High-throughput RNA-seq Library Preparation for Illumina Sequencing from Plant Tissue
    植物组织中用于Illumina测序的低成本、高通量RNA-seq文库制备
    作者:Marta Bjornson, Kaisa Kajala, Cyril Zipfel and Pingtao Ding日期:10/20/2020,浏览量:354,Q&A: 0
    Transcriptome analysis can provide clues to biological processes affected in different genetic backgrounds or/and under various conditions. The price of RNA sequencing (RNA-seq) has decreased enough so that medium- to large-scale transcriptome ...
    Quantification of Methylglyoxal Levels in Cowpea Leaves in Response to Cowpea Aphid Infestation
    豇豆蚜虫侵染对豇豆叶片中甲基乙二醛含量的定量影响
    Aphids are a serious pest of crops across the world. Aphids feed by inserting their flexible hypodermal needlelike mouthparts, or stylets, into their host plant tissues. They navigate their way to the phloem where they feed on its sap causing little ...
    Dual sgRNA-based Targeted Deletion of Large Genomic Regions and Isolation of Heritable Cas9-free Mutants in Arabidopsis
    基于双sgRNA的拟南芥大基因组区域靶向清除及可遗传的非Cas9突变体的分离
    作者:Yu Jin and Sebastian Marquardt日期:10/20/2020,浏览量:239,Q&A: 0
    CRISPR/Cas9 system directed by a gene-specific single guide RNA (sgRNA) is an effective tool for genome editing such as deletions of few bases in coding genes. However, targeted deletion of larger regions generate loss-of-function alleles ...
    Acidified Blue Ink-staining Procedure for the Observation of Fungal Structures Inside Roots of Two Disparate Plant Lineages
    两个不同植物谱系根内真菌结构的酸化蓝墨水染色法观察流程
    作者:Jill Kowal, Elena Arrigoni and Sophie Lane日期:10/20/2020,浏览量:189,Q&A: 0
    [Abstract] Identifying microscopic mycorrhizal fungal structures in roots, i.e., hyphae, vesicles and arbuscules, requires root staining procedures that are often time consuming and involves chemicals known to present health risks from exposure. By modifying established protocols, our root staining method stains roots using a safe ink- and ...
    Low-cost and High-throughput RNA-seq Library Preparation for Illumina Sequencing from Plant Tissue
    植物组织中用于Illumina测序的低成本、高通量RNA-seq文库制备
    作者:Marta Bjornson, Kaisa Kajala, Cyril Zipfel and Pingtao Ding日期:10/20/2020,浏览量:354,Q&A: 0
    [Abstract] Transcriptome analysis can provide clues to biological processes affected in different genetic backgrounds or/and under various conditions. The price of RNA sequencing (RNA-seq) has decreased enough so that medium- to large-scale transcriptome analyses in a range of conditions are feasible. However, the price and variety of options for library ...
    Quantification of Methylglyoxal Levels in Cowpea Leaves in Response to Cowpea Aphid Infestation
    豇豆蚜虫侵染对豇豆叶片中甲基乙二醛含量的定量影响
    [Abstract] Aphids are a serious pest of crops across the world. Aphids feed by inserting their flexible hypodermal needlelike mouthparts, or stylets, into their host plant tissues. They navigate their way to the phloem where they feed on its sap causing little mechanical damage to the plant. Additionally, while feeding, aphids secrete proteinaceous effectors ...
    Dual sgRNA-based Targeted Deletion of Large Genomic Regions and Isolation of Heritable Cas9-free Mutants in Arabidopsis
    基于双sgRNA的拟南芥大基因组区域靶向清除及可遗传的非Cas9突变体的分离
    作者:Yu Jin and Sebastian Marquardt日期:10/20/2020,浏览量:239,Q&A: 0
    [Abstract] CRISPR/Cas9 system directed by a gene-specific single guide RNA (sgRNA) is an effective tool for genome editing such as deletions of few bases in coding genes. However, targeted deletion of larger regions generate loss-of-function alleles that offer a straightforward starting point for functional dissections of genomic loci. We present an ...
    Multitarget Immunohistochemistry for Confocal and Super-resolution Imaging of Plant Cell Wall Polysaccharides
    植物细胞壁多糖共聚焦和超分辨成像的多靶点免疫组织化学研究
    作者:Kalina T. Haas, Methieu Rivière, Raymond Wightman and Alexis Peaucelle日期:10/05/2020,浏览量:573,Q&A: 0
    [Abstract] The plant cell wall (PCW) is a pecto-cellulosic extracellular matrix that envelopes the plant cell. By integrating extra-and intra-cellular cues, PCW mediates a plethora of essential physiological functions. Notably, it permits controlled and oriented tissue growth by tuning its local mechano-chemical properties. To refine our knowledge of these ...
    A Protocol for Flavonols, Kaempferol and Quercetin, Staining in Plant Root Tips
    植物根尖中黄酮醇、山奈酚和槲皮素的染色方法
    作者:Nguyen Hoai Nguyen日期:10/05/2020,浏览量:371,Q&A: 0
    [Abstract] Flavonols are a subclass of flavonoids of the group of plant secondary metabolites. In planta, flavonols play various functions such as antioxidant and natural regulator of auxin polar transport. Many lines of evidence have shown that flavonols also contribute to human health in anti-oxidation, anti-inflammation, and even prevention some ...
    Expression and Purification of Arabidopsis Transmembrane Protein BCM1 in Saccharomyces cerevisiae
    拟南芥跨膜蛋白BCM1在酿酒酵母中的表达与纯化
    作者:Peng Wang and Bernhard Grimm日期:09/20/2020,浏览量:319,Q&A: 0
    [Abstract] Heterologous expression and purification of transmembrane proteins have remained a challenge for decades hampering detailed biochemical and structural characterization of key enzymes and their interacting regulators in multiple metabolic pathways. An in-depth study on the newly identified Arabidopsis thaliana integral membrane protein ...
    Production and Bioassay of a Diffusible Factor that Induces Gametophyte-to-Sporophyte Developmental Reprogramming in the Brown Alga Ectocarpus
    水云属褐藻配子体-孢子体发育重编程扩散因子的产生及生物测定
    [Abstract] The brown alga Ectocarpus has a haploid-diploid life cycle that involves alternation between two multicellular generations, the sporophyte and the gametophyte. Life cycle generation is not determined by ploidy but by a genetic system that includes two different three amino acid loop extension homeodomain transcription factors called ...
    Efficient Agrobacterium-mediated Transformation of the Elite–Indica Rice Variety Komboka
    农杆菌介导的优良籼稻品种Komboka的高效转化
    [Abstract] Genetic transformation is crucial for both investigating gene functions and for engineering of crops to introduce new traits. Rice (Oryza sativa L.) is an important model in plant research, since it is the staple food for more than half of the world’s population. As a result, numerous transformation methods have been developed for both ...
    An Efficient Inoculation Technique to Assess the Pathogenicity of Pantoea Species Associated to Bacterial Blight of Rice
    一种用于评估水稻白叶枯病泛菌致病性的高效接种技术
    作者:Kossi Kini, Raoul Agnimonhan, Issa Wonni and Drissa Silue日期:09/05/2020,浏览量:646,Q&A: 0
    [Abstract] Bacteria blight diseases of rice due to several genera of pathogenic bacteria are one of the major constraints worldwide for rice production. The disease can be best managed through host plant resistance sources. For most of these bacteria such as Xanthomonas oryzae pv. oryzae, X. oryzae pv. oryzicola, ...