生物化学

分类

    现刊
    In vitro RNA Cleavage Assays to Characterize IRE1-dependent RNA Decay
    通过体外RNA剪切检测IRE1依赖的RNA降解
    作者:G. Elif Karagöz, Jirka Peschek, Peter Walter and Diego Acosta-Alvear日期:07/20/2019,浏览量:11,Q&A: 0
    The kinase/RNase IRE1 is a key effector of the cellular response to endoplasmic reticulum stress. The RNase activity of IRE1 can be measured in cells or in the test tube. Here we describe a protocol for the in vitro cleavage and analysis of ...
    Isoelectric Focusing to Quantify Rhodopsin Phosphorylation in Mouse Retina
    等电聚焦用于小鼠视网膜中磷酸化视紫红质的定量分析
    作者:Sowmya Bekshe Lokappa, M. Carter Cornwall and Jeannie Chen日期:07/20/2019,浏览量:14,Q&A: 0
    Rhodopsin is a G-protein coupled receptor (GPCR) that mediates vision under dim light. Upon light exposure, rhodopsin is phosphorylated at multiple serine and threonine sites at its carboxyl-terminus by rhodopsin kinase (GRK1). This, in turn, ...
    Evaluation of Genotoxicity by Micronucleus Assay in vitro and by Allium cepa Test in vivo
    利用微核体外分析系统和洋葱体内实验进行遗传毒性测试
    作者:Christina N. Banti and Sotiris K. Hadjikakou日期:07/20/2019,浏览量:18,Q&A: 0
    The in vitro and in vivo genotoxicity of new metallodrugs either as Small Bioactive Molecules (SBAMs) or Conjugates of Metals with Drugs (CoMeDs) is evaluated by the micronucleus test and the Allium cepa assay, ...
    In vitro RNA Cleavage Assays to Characterize IRE1-dependent RNA Decay
    通过体外RNA剪切检测IRE1依赖的RNA降解
    作者:G. Elif Karagöz, Jirka Peschek, Peter Walter and Diego Acosta-Alvear日期:07/20/2019,浏览量:11,Q&A: 0
    [Abstract] The kinase/RNase IRE1 is a key effector of the cellular response to endoplasmic reticulum stress. The RNase activity of IRE1 can be measured in cells or in the test tube. Here we describe a protocol for the in vitro cleavage and analysis of RNA substrates of IRE1. The method consists of the in vitro transcription, purification ...
    Isoelectric Focusing to Quantify Rhodopsin Phosphorylation in Mouse Retina
    等电聚焦用于小鼠视网膜中磷酸化视紫红质的定量分析
    作者:Sowmya Bekshe Lokappa, M. Carter Cornwall and Jeannie Chen日期:07/20/2019,浏览量:14,Q&A: 0
    [Abstract] Rhodopsin is a G-protein coupled receptor (GPCR) that mediates vision under dim light. Upon light exposure, rhodopsin is phosphorylated at multiple serine and threonine sites at its carboxyl-terminus by rhodopsin kinase (GRK1). This, in turn, reduces its ability to activate the visual G-protein transducin. Binding of light-activated, ...
    Evaluation of Genotoxicity by Micronucleus Assay in vitro and by Allium cepa Test in vivo
    利用微核体外分析系统和洋葱体内实验进行遗传毒性测试
    作者:Christina N. Banti and Sotiris K. Hadjikakou日期:07/20/2019,浏览量:18,Q&A: 0
    [Abstract] The in vitro and in vivo genotoxicity of new metallodrugs either as Small Bioactive Molecules (SBAMs) or Conjugates of Metals with Drugs (CoMeDs) is evaluated by the micronucleus test and the Allium cepa assay, respectively. Fetal lung fibroblast cells (MRC-5), normal human corneal epithelial cells (HCEC) and ...
    Adhesive and Cohesive Peel Force Measurement of Human Airway Mucus
    人气道粘液的黏附力和粘结剥离力测定
    [Abstract] In health, the high-speed airflow associated with cough represents a vital backup mechanism for clearing accumulated mucus from our airways. However, alterations in the mucus layer in cystic fibrosis (CF) and chronic obstructive pulmonary disease (COPD) leads to the mucus layer adhered to the airway surfaces, representing the nidus of chronic lung ...
    Oxygen Consumption Measurements in Caenorhabditis elegans Using the Seahorse XF24
    利用海马细胞能量代谢实时测定仪XF24 进行秀丽隐杆线虫中耗氧量测定
    作者:Suraiya Haroon and Marc Vermulst日期:07/05/2019,浏览量:179,Q&A: 0
    [Abstract] Mitochondria generate 90% of the energy required to sustain life. As a result, loss of mitochondrial function compromises almost every facet of human physiology. Accordingly, most mitochondrial diseases tend to present themselves as complex, multi-systemic disorders that can be difficult to diagnose. Depending on the severity of the mitochondrial ...
    In vitro and in vivo Assessment of Protein Acetylation Status in Mycobacteria
    分枝杆菌中蛋白乙酰化状态的体外和体内检测
    作者:Krishna K. Singh, Devendra P. Singh, Rambir Singh and Deepak K. Saini日期:07/05/2019,浏览量:205,Q&A: 0
    [Abstract] Protein acetylation is one of the standard post-translational modifications found in proteins across all organisms, along with phosphorylation which regulates diverse cellular processes. Acetylation of proteins can be enzymatically catalyzed through acetyltransferases, acetyl CoA synthetases or non-enzymatically through acyl carrier metabolic ...
    Preparation and Purification of Active Recombinant Human Pancreatic Lipase in Escherichia coli
    重组人胰脂肪酶在大肠杆菌中的表达和纯化
    作者:Nanami Kawaguchi, Haruko Ogawa and Kimie Date日期:07/05/2019,浏览量:194,Q&A: 0
    [Abstract] Human pancreatic lipase (HPL) is the main lipolytic enzyme involved in the digestion of dietary fat. An active recombinant human pancreatic lipase (recHPL) was successfully prepared for the first time in an Escherichia coli (E. coli) expression system using a short Strep-tag II (ST II). The recHPL-ST II was solubilized with 8 M urea from ...
    Capillary Nano-immunoassay for Quantification of Proteins from CD138-purified Myeloma Cells
    毛细管纳米免疫实验定量分析经CD138筛选的骨髓瘤细胞蛋白
    作者:Irena Misiewicz-Krzeminska, Isabel Isidro and Norma C. Gutiérrez日期:06/20/2019,浏览量:995,Q&A: 0
    [Abstract] Protein analysis in bone marrow samples from patients with multiple myeloma (MM) has been limited by the low concentration of proteins obtained after CD138+ cell selection. A novel approach based on capillary nano-immunoassay could make it possible to quantify dozens of proteins from each CD138+ purified MM sample in an ...
    Fluorescence HPLC Analysis of the in-vivo Activity of Glucosylceramide Synthase
    葡糖基神经酰胺合酶体内活性的荧光HPLC检测
    作者:Kartik R. Roy, Sachin K. Khiste, Zhijun Liu and Yong-Yu Liu日期:06/20/2019,浏览量:955,Q&A: 0
    [Abstract] Almost all functions of cells or organs rely on the activities of cellular enzymes. Indeed, the in-vivo activities that directly represent the cellular effects of enzymes in live organs are critical importance to appreciate the roles enzymes play in modulating physiological or pathological processes, although assessments of such in-vivo ...
    Probing Conformational States of a Target Protein in Escherichia coli Cells by in vivo Cysteine Cross-linking Coupled with Proteolytic Gel Analysis
    利用体内半胱氨酸交联结合蛋白水解凝胶分析检测大肠杆菌细胞中目的蛋白的构象状态
    作者:Sujeet Kumar and Natividad Ruiz 日期:06/20/2019,浏览量:278,Q&A: 0
    [Abstract] Transporters are dynamic membrane proteins that are essential to the physiology of cells. To function, transporters must cycle between various conformational states, so to understand their mechanistic details, it is critical to characterize how their structure changes during the transport cycle. One approach to studying the dynamics of ...