分子生物学

分类

    现刊
    Novel Protein-oligonucleotide Conjugation Method Involving a High-affinity Capture HaloTag
    一种高亲和力捕获卤代烷脱氢酶标记的蛋白质寡核苷酸偶联新方法
    作者:Junshi Yazaki日期:09/20/2020,浏览量:62,Q&A: 0
    Highly sensitive quantitative protein profiling can play a key role in the early diagnosis of diseases, such as autoimmune diseases and cancer. We developed a modified protein-oligonucleotide conjugation method termed HaloTag-mediated barcoding, for ...
    Quantification of The Surface Expression of G Protein-coupled Receptors Using Intact Live-cell Radioligand Binding Assays
    利用完整活细胞放射配体结合分析定量G蛋白偶联受体的表面表达
    作者:Xin Xu and Guangyu Wu日期:09/20/2020,浏览量:64,Q&A: 0
    G protein-coupled receptors (GPCRs) are the most structurally diverse family of signaling proteins and regulate a variety of cell function. For most GPCRs, the cell surface is their functional destination where they are able to respond a wide range ...
    A Workflow for Ultra-rapid Analysis of Histone Post-translational Modifications with Direct-injection Mass Spectrometry
    使用直接进样质谱对组蛋白翻译后修饰进行超快速分析的工作流程
    作者:Natarajan V. Bhanu, Simone Sidoli and Benjamin A Garcia日期:09/20/2020,浏览量:63,Q&A: 0
    Chromatin modifications, like histone post translational modifications (PTMs), are critical for tuning gene expression and many other aspects of cell phenotype. Liquid chromatography coupled to mass spectrometry (LC-MS) has become the most suitable ...
    Immunohistochemistry of Kidney a-SMA, Collagen 1, and Collagen 3, in A Novel Mouse Model of Reno-cardiac Syndrome
    心肾综合征新型小鼠模型中肾脏a-SMA,胶原1和胶原3的免疫组化研究
    Cardiorenal syndrome defines a synergistic pathology of the heart and kidneys where failure of one organ causes failure in the other. The incidence of cardiovascular mortality caused by this syndrome, is 20 fold higher in the end stage renal disease ...
    Novel Protein-oligonucleotide Conjugation Method Involving a High-affinity Capture HaloTag
    一种高亲和力捕获卤代烷脱氢酶标记的蛋白质寡核苷酸偶联新方法
    作者:Junshi Yazaki日期:09/20/2020,浏览量:62,Q&A: 0
    [Abstract] Highly sensitive quantitative protein profiling can play a key role in the early diagnosis of diseases, such as autoimmune diseases and cancer. We developed a modified protein-oligonucleotide conjugation method termed HaloTag-mediated barcoding, for quantifying protein molecules at a higher sensitivity than conventional protein quantification ...
    Quantification of The Surface Expression of G Protein-coupled Receptors Using Intact Live-cell Radioligand Binding Assays
    利用完整活细胞放射配体结合分析定量G蛋白偶联受体的表面表达
    作者:Xin Xu and Guangyu Wu日期:09/20/2020,浏览量:64,Q&A: 0
    [Abstract] G protein-coupled receptors (GPCRs) are the most structurally diverse family of signaling proteins and regulate a variety of cell function. For most GPCRs, the cell surface is their functional destination where they are able to respond a wide range of extracellular stimuli, leading to the activation of intracellular signal transduction cascades. ...
    A Workflow for Ultra-rapid Analysis of Histone Post-translational Modifications with Direct-injection Mass Spectrometry
    使用直接进样质谱对组蛋白翻译后修饰进行超快速分析的工作流程
    作者:Natarajan V. Bhanu, Simone Sidoli and Benjamin A Garcia日期:09/20/2020,浏览量:63,Q&A: 0
    [Abstract] Chromatin modifications, like histone post translational modifications (PTMs), are critical for tuning gene expression and many other aspects of cell phenotype. Liquid chromatography coupled to mass spectrometry (LC-MS) has become the most suitable method to analyze histones and histone PTMs in a large-scale manner. Selected histone PTMs have ...
    Immunohistochemistry of Kidney a-SMA, Collagen 1, and Collagen 3, in A Novel Mouse Model of Reno-cardiac Syndrome
    心肾综合征新型小鼠模型中肾脏a-SMA,胶原1和胶原3的免疫组化研究
    [Abstract] Cardiorenal syndrome defines a synergistic pathology of the heart and kidneys where failure of one organ causes failure in the other. The incidence of cardiovascular mortality caused by this syndrome, is 20 fold higher in the end stage renal disease (ESRD) population compared to the population as a whole thus necessitating the need for improved ...
    Nonenzymatic RNA-templated Synthesis of N3′→P5′ Phosphoramidate DNA
    N3'→P5'磷酰胺DNA的非酶RNA模板合成
    作者:Derek K. O'Flaherty, Lijun Zhou and Jack W. Szostak日期:09/05/2020,浏览量:369,Q&A: 0
    [Abstract] The RNA world hypothesis describes a scenario where early life forms relied on RNA to govern both inheritance and catalyze useful chemical reactions. Prior to the emergence of enzymes capable of replicating the RNA genome, a nonenzymatic replication process would have been necessary to initiate Darwinian Evolution. However, the one-pot ...
    Screening Method for CRISPR/Cas9 Inhibition of a Human DNA Virus: Herpes Simplex Virus
    人单纯疱疹病毒CRISPR/Cas9抑制作用的筛选方法
    [Abstract] The efficiency of cleavage of individual CRISPR/Cas9-sgRNAs remains difficult to predict based on the CRISPR target sequence alone. Different intracellular environments (dependent on cell type or cell cycle state for example) may affect sgRNA efficiency by altering accessibility of genomic DNA through DNA modifications such as epigenetic marks and ...
    BRIDGE: An Open Platform for Reproducible Protein-Ligand Simulations and Free Energy of Binding Calculations
    BRIDGE:一个可重复的蛋白质配体模拟和结合自由能计算的开放平台
    作者:Tharindu Senapathi, Christopher B. Barnett and Kevin J. Naidoo日期:09/05/2020,浏览量:771,Q&A: 0
    [Abstract] Protein-ligand binding prediction is central to the drug-discovery process. This often follows an analysis of genomics data for protein targets and then protein structure discovery. However, the complexity of performing reproducible protein conformational analysis and ligand binding calculations, using vetted methods and protocols can be a ...
    Ratiometric Measurement of Protein Abundance after Transient Expression of a Transgene in Nicotiana benthamiana
    转基因烟草瞬时表达后蛋白质丰度的比例计量测定
    作者:Aashima Khosla and David C. Nelson日期:09/05/2020,浏览量:484,Q&A: 0
    [Abstract] Ratiometric reporters are tools to dynamically measure the relative abundance of a protein of interest. In these systems, a target protein fused to a fluorescent or bioluminescent reporter is expressed with fixed stoichiometry to a reference protein fused to a second reporter. Both fusion proteins are encoded on a single transcript but are ...
    Microtubule Seeded-assembly in the Presence of Poorly Nucleating Nucleotide Analogues
    存在不良成核核苷酸类似物的微管种晶组装
    作者:Siou Ku, Claire Heichette, Laurence Duchesne and Denis Chrétien日期:08/20/2020,浏览量:469,Q&A: 0
    [Abstract] Microtubule dynamic instability is driven by the hydrolysis of the GTP bound to the β-subunit of the α-β tubulin heterodimer. Nucleotide analogues are commonly used to mimic the different steps of the tubulin GTPase cycle, but most of them are poor microtubule nucleators. Usually, microtubule assembly is seeded by guanylyl-(α, ...
    Mapping mRNA-18S rRNA Contacts Within Translation Initation Complex by Means of Reverse Transcriptase Termination Sites and RNAseq
    用逆转录酶终止位点和RNAseq定位翻译起始复合体内的mRNA-18S rRNA接触点
    作者:Irene Díaz-López, René Toribio and Iván Ventoso日期:08/20/2020,浏览量:572,Q&A: 0
    [Abstract] The nucleotides involved in RNA-RNA interaction can be tagged by chemical- or UV-induced crosslinking, and further identified by classical or modern high throughput techniques. The contacts of mRNA with 18S rRNA that occur along the mRNA channel of 40S subunit have been mapped by site-specific UV crosslinking followed by reverse transcriptase ...