分子生物学

分类

    现刊
    Preparation of Yeast tRNA Sample for NMR Spectroscopy
    酵母tRNA样品的核磁共振制备
    作者:Marjorie Catala, Alexandre Gato, Carine Tisné and Pierre Barraud日期:06/20/2020,浏览量:444,Q&A: 0
    Transfer RNAs (tRNAs) are heavily decorated with post-transcriptional modifications during their biosynthesis. To fulfil their functions within cells, tRNAs undergo a tightly controlled biogenesis process leading to the formation of mature tRNAs. In ...
    Superresolution Microscopy of Drosophila Indirect Flight Muscle Sarcomeres
    果蝇间接飞行肌肌节的超分辨显微镜观察
    作者:Szilárd Szikora, Tibor Novák, Tamás Gajdos, Miklós Erdélyi and József Mihály日期:06/20/2020,浏览量:450,Q&A: 0
    Sarcomeres are extremely highly ordered macromolecular assemblies where proper structural organization is an absolute prerequisite to the functionality of these contractile units. Despite the wealth of information collected, the exact spatial ...
    Site-specific DNA Mapping of Protein Binding Orientation Using Azidophenacyl Bromide (APB)
    叠氮苯酰溴(APB)定位蛋白质结合方向的DNA图谱
    作者:Himasha M. Perera and Michael A. Trakselis日期:06/20/2020,浏览量:620,Q&A: 0
    The orientation of a DNA-binding protein bound on DNA is determinative in directing the assembly of other associated proteins in the complex for enzymatic action. As an example, in a replisome, the orientation of the DNA helicase at the replication ...
    Electrophoretic Mobility Shift Assay of in vitro Phosphorylated RNA Polymerase II Carboxyl-terminal Domain Substrates
    体外磷酸化的RNA聚合酶II羧基末端域底物的电泳迁移率测定
    作者:Joshua E. Mayfield, Seema Irani and Yan Zhang日期:06/20/2020,浏览量:638,Q&A: 0
    Eukaryotic RNA polymerase II transcribes all protein-coding mRNAs and is highly regulated. A key mechanism directing RNA polymerase II and facilitating the co-transcriptional processing of mRNAs is the phosphorylation of its highly repetitive ...
    Low-cost and Multiplexable Whole mRNA-Seq Library Preparation Method with Oligo-dT Magnetic Beads for Illumina Sequencing Platforms
    Oligo-dT磁珠用于Illumina测序平台的低成本和可复用的完整mRNA-Seq文库制备方法
    作者:Makoto Kashima, Ayumi Deguchi, Ayumi Tezuka and Atsushi J. Nagano日期:06/20/2020,浏览量:882,Q&A: 0
    RNA-Seq is a powerful method for transcriptome analysis used in varied field of biology. Although several commercial products and hand-made protocols enable us to prepare RNA-Seq library from total RNA, their cost are still expensive. Here, we ...
    Quantitative Nucleocytoplasmic Transport Assays in Cellular Models of Neurodegeneration
    神经退行性细胞模型中核质转运的定量分析
    作者:Joni Vanneste, Thomas Vercruysse, Philip Van Damme, Ludo Van Den Bosch and Dirk Daelemans日期:06/20/2020,浏览量:444,Q&A: 0
    Nucleocytoplasmic transport deficits are suggested to play a role in neurodegenerative disorders, including amyotrophic lateral sclerosis (ALS). Given the importance and complexity of this process, understanding when these aberrations occur and ...
    Genomic Edition of Ashbya gossypii Using One-vector CRISPR/Cas9
    利用一个CRISPR/Cas9载体对棉囊阿舒氏酵母进行基因组编辑
    作者:Gloria Muñoz-Fernández, Alberto Jiménez and José Luis Revuelta日期:06/20/2020,浏览量:371,Q&A: 0
    The CRISPR/Cas9 system is a novel genetic tool which allows the precise manipulation of virtually any genomic sequence. In this protocol, we use a specific CRISPR/Cas9 system for the manipulation of Ashbya gossypii. The filamentous fungus ...
    Preparation of Yeast tRNA Sample for NMR Spectroscopy
    酵母tRNA样品的核磁共振制备
    作者:Marjorie Catala, Alexandre Gato, Carine Tisné and Pierre Barraud日期:06/20/2020,浏览量:444,Q&A: 0
    [Abstract] Transfer RNAs (tRNAs) are heavily decorated with post-transcriptional modifications during their biosynthesis. To fulfil their functions within cells, tRNAs undergo a tightly controlled biogenesis process leading to the formation of mature tRNAs. In addition, functions of tRNAs are often modulated by their modifications. Although the biological ...
    Superresolution Microscopy of Drosophila Indirect Flight Muscle Sarcomeres
    果蝇间接飞行肌肌节的超分辨显微镜观察
    作者:Szilárd Szikora, Tibor Novák, Tamás Gajdos, Miklós Erdélyi and József Mihály日期:06/20/2020,浏览量:450,Q&A: 0
    [Abstract] Sarcomeres are extremely highly ordered macromolecular assemblies where proper structural organization is an absolute prerequisite to the functionality of these contractile units. Despite the wealth of information collected, the exact spatial arrangement of many of the H-zone and Z-disk proteins remained unknown. Recently, we developed a powerful ...
    Site-specific DNA Mapping of Protein Binding Orientation Using Azidophenacyl Bromide (APB)
    叠氮苯酰溴(APB)定位蛋白质结合方向的DNA图谱
    作者:Himasha M. Perera and Michael A. Trakselis日期:06/20/2020,浏览量:620,Q&A: 0
    [Abstract] The orientation of a DNA-binding protein bound on DNA is determinative in directing the assembly of other associated proteins in the complex for enzymatic action. As an example, in a replisome, the orientation of the DNA helicase at the replication fork directs the assembly of the other associated replisome proteins. We have recently determined ...
    Electrophoretic Mobility Shift Assay of in vitro Phosphorylated RNA Polymerase II Carboxyl-terminal Domain Substrates
    体外磷酸化的RNA聚合酶II羧基末端域底物的电泳迁移率测定
    作者:Joshua E. Mayfield, Seema Irani and Yan Zhang日期:06/20/2020,浏览量:638,Q&A: 0
    [Abstract] Eukaryotic RNA polymerase II transcribes all protein-coding mRNAs and is highly regulated. A key mechanism directing RNA polymerase II and facilitating the co-transcriptional processing of mRNAs is the phosphorylation of its highly repetitive carboxyl-terminal domain (CTD) of its largest subunit, RPB1, at specific residues. A variety of techniques ...
    Low-cost and Multiplexable Whole mRNA-Seq Library Preparation Method with Oligo-dT Magnetic Beads for Illumina Sequencing Platforms
    Oligo-dT磁珠用于Illumina测序平台的低成本和可复用的完整mRNA-Seq文库制备方法
    作者:Makoto Kashima, Ayumi Deguchi, Ayumi Tezuka and Atsushi J. Nagano日期:06/20/2020,浏览量:882,Q&A: 0
    [Abstract] RNA-Seq is a powerful method for transcriptome analysis used in varied field of biology. Although several commercial products and hand-made protocols enable us to prepare RNA-Seq library from total RNA, their cost are still expensive. Here, we established a low-cost and multiplexable whole mRNA-Seq library preparation method for illumine ...
    Quantitative Nucleocytoplasmic Transport Assays in Cellular Models of Neurodegeneration
    神经退行性细胞模型中核质转运的定量分析
    作者:Joni Vanneste, Thomas Vercruysse, Philip Van Damme, Ludo Van Den Bosch and Dirk Daelemans日期:06/20/2020,浏览量:444,Q&A: 0
    [Abstract] Nucleocytoplasmic transport deficits are suggested to play a role in neurodegenerative disorders, including amyotrophic lateral sclerosis (ALS). Given the importance and complexity of this process, understanding when these aberrations occur and which pathways are involved is of great importance. Here, we make use of CRISPR-Cas9 technology to ...
    Genomic Edition of Ashbya gossypii Using One-vector CRISPR/Cas9
    利用一个CRISPR/Cas9载体对棉囊阿舒氏酵母进行基因组编辑
    作者:Gloria Muñoz-Fernández, Alberto Jiménez and José Luis Revuelta日期:06/20/2020,浏览量:371,Q&A: 0
    [Abstract] The CRISPR/Cas9 system is a novel genetic tool which allows the precise manipulation of virtually any genomic sequence. In this protocol, we use a specific CRISPR/Cas9 system for the manipulation of Ashbya gossypii. The filamentous fungus A. gossypii is currently used for the industrial production of riboflavin (vitamina B2). In ...
    Biochemical Pulldown of mRNAs and Long Noncoding RNAs from Cellular Lysates Coupled with Mass Spectrometry to Identify Protein Binding Partners
    通过细胞裂解液中mRNAs和长链非编码RNA的生化沉降结合质谱法鉴定蛋白结合物
    作者:Anca F. Savulescu, Stoyan Stoychev, Sipho Mamputha and Musa M. Mhlanga日期:06/05/2020,浏览量:795,Q&A: 0
    [Abstract] RNA binding proteins (RBPs) interact with cellular mRNAs, controlling various steps throughout the lifetime of these transcripts, including transcription, cellular transport, subcellular localization, translation and degradation. In addition to binding mRNA transcripts, a growing number of RBPs are shown to bind long noncoding RNAs (lncRNAs), ...
    Extracellular Vesicles Tracking and Quantification Using CT and Optical Imaging in Rats
    大鼠细胞外囊泡的CT和光学成像追踪与定量研究
    作者:Shaowei Guo, Oshra Betzer, Nisim Perets, Shira Landau, Daniel Offen, Rachela Popovtzer and Shulamit Levenberg日期:06/05/2020,浏览量:800,Q&A: 0
    [Abstract] Exosomes, a subtype of extracellular vesicles, are nanovesicles of endocytic origin. Exosomes contain a plethora of proteins, lipids, and genetic materials of parent cells to facilitate intercellular communications. Tracking exosomes in vivo is fundamentally important to understand their biodistribution pattern and the mechanism of ...
    Mouse Adipose Tissue Protein Extraction
    小鼠脂肪组织蛋白提取
    作者:Yu A. An and Philipp E. Scherer日期:06/05/2020,浏览量:655,Q&A: 0
    [Abstract] As obesity becomes a global epidemic, the metabolism research field is increasingly focusing on studying the physiological and pathological roles of adipose tissues (AT). However, extracting proteins from AT is challenging due to abundant fat content of intracellular lipid droplets. Several commercial kits for extraction of AT proteins are ...