• 卷册 9, 2019
  • 卷册 8, 2018
  • 卷册 7, 2017
  • 卷册 6, 2016
  • 卷册 5, 2015
  • 卷册 4, 2014
  • 卷册 3, 2013
  • 卷册 2, 2012
  • 卷册 1, 2011

现期刊物(2019)

卷册: 9, 期号: 9

生物化学

Optimized Oxidative Stress Protocols for Low-microliter Volumes of Mammalian Plasma 可用于测定低微量哺乳动物血浆中氧化应激因子的优化实验方法 作者:Evan A. Langille日期:05/05/2019,浏览量:186,Q&A: 0
Small blood volumes commonly obtained from small mammals during field studies are only sufficient for a single biochemical assay. In this study, we used blood collected from a population of wild eastern chipmunks (Tamias striatus) and developed modified methods to improve analytical selectivity and sensitivity required for measuring ...
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Separation of Natural Collagen Crosslinks Using Buffer and Ion-pairing Agent Free Solvents on Silica Hydride Column for Mass Spectrometry Detection 二氧化硅氢化物柱配合使用缓冲液和离子配对剂分离获得可通过质谱检测交联的天然胶原蛋白 作者:Rafea Naffa and Joseph Pesek日期:05/05/2019,浏览量:185,Q&A: 0
In this protocol we describe the separation of collagen crosslinks in biological tissues and samples including skin, tendon, cartilage, bone and urine. The existing methods use either cation exchange chromatography followed by post-column derivatization with ninhydrin or reverse phase chromatography with mass spectrometry detection. The cation ...
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癌症生物学

3D Co-culture System of Mouse Prostatic Wild-type Fibroblasts with Human Prostate Cancer Epithelial Cells 小鼠前列腺野生型成纤维细胞和人前列腺癌上皮细胞的3D共培养系统 作者:Veronica R. Placencio-Hickok, Manabu Kato and Neil A. Bhowmick日期:05/05/2019,浏览量:258,Q&A: 0
Heterogeneous prostatic carcinoma-associated fibroblasts (CAF) contribute to tumor progression. This was established using transgenic mouse models. Paracrine interactions between fibroblasts and epithelial cells were further interrogated using isolated 2D cell culture systems, but 3D culture systems currently being developed can better mimic ...
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细胞生物学

Triple Fluorescence Anisotropy Reporter Imaging in Living Cells 三荧光各向异性报告基因活细胞成像 作者:Brian Ross, Shenq Huey Wong, Nicole E. Snell, Jin Zhang and M. A. Rizzo日期:05/05/2019,浏览量:233,Q&A: 0
FRET-based genetically encoded biosensors incorporate two fluorescent proteins into their design to enable ratiometric biosensing of signaling activities in live cells. While emission ratios are generally useful for quantitative studies, they leave little room in the optical spectrum for additional sensors and optogenetic tools. Homotransfer-based ...
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Quantification of Cutaneous Ionocytes in Small Aquatic Organisms 小型水生生物皮肤离子细胞的定量分析 作者:Garfield T. Kwan, Shane H. Finnerty, Nicholas C. Wegner and Martin Tresguerres日期:05/05/2019,浏览量:204,Q&A: 0
Aquatic organisms have specialized cells called ionocytes that regulate the ionic composition, osmolarity, and acid/base status of internal fluids. In small aquatic organisms such as fishes in their early life stages, ionocytes are typically found on the cutaneous surface and their abundance can change to help cope with various metabolic and ...
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微生物学

Analysis of Indole-3-acetic Acid (IAA) Production in Klebsiella by LC-MS/MS and the Salkowski Method 利用液相色谱-串联质谱法和萨尔科夫斯基氏法分析克雷伯菌中吲哚乙酸的产生 作者:Shraddha Gang, Sheetal Sharma, Meenu Saraf, Martin Buck and Jörg Schumacher日期:05/05/2019,浏览量:193,Q&A: 0
Many rhizobacteria isolated from plant rhizosphere produce various phytohormones in the form of secondary metabolites, the most common of which is Indole-3-acetic acid (IAA). Here, we detail analytical protocols of IAA detection and quantification, in vitro and in situ, as recently applied to Klebsiella SGM 81, a ...
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分子生物学

Improved HTGTS for CRISPR/Cas9 Off-target Detection 改进的HTGTS用于 CRISPR/Cas9脱靶检测 作者:Jianhang Yin, Mengzhu Liu, Yang Liu and Jiazhi Hu日期:05/05/2019,浏览量:129,Q&A: 0
Precise genome editing is essential for scientific research and clinical application. At present, linear amplification-mediated high-throughput genome-wide translocation sequencing (LAM-HTGTS) is one of most effective methods to evaluate the off-target activity of CRISPR-Cas9, which is based on chromosomal translocation and employs a “bait” DNA ...
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神经科学

Inducing Alcohol Dependence in Rats Using Chronic Intermittent Exposure to Alcohol Vapor 长期间歇性暴露于酒精蒸汽以诱导大鼠酒精依赖 作者:Elizabeth M. Avegno and Nicholas W. Gilpin日期:05/05/2019,浏览量:133,Q&A: 0
Alcohol use disorder (AUD) is a significant public health and economic burden and is often characterized by repeated bouts of alcohol intoxication and withdrawal. Virtually all organ systems are impacted by chronic alcohol exposure. These effects can be investigated using the rat as a model organism; however, rats typically will not ...
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植物科学

Determination of Root Exudate Concentration in the Rhizosphere Using 13C Labeling 13C标记测定根际根系分泌物浓度 作者:Laurent Simon and Feth el Zahar Haichar日期:05/05/2019,浏览量:156,Q&A: 0
One of the most remarkable metabolic features of plant roots is their ability to secrete a wide range of compounds into the rhizosphere, defined as the volume of soil around living roots. Around 5%-21% of total photosynthetically fixed carbon is transferred into the rhizosphere through root exudates. Until recently, studies on the quantity and ...
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