• 卷册 11, 2021
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Image Credit: BioProtoc.3930

现期刊物(2021)

卷册: 11, 期号: 4

生物化学

Expression and Purification of Yeast-derived GPCR, Gα and Gβγ Subunits for Structural and Dynamic Studies 重组酵母的GPCR、Gα和Gβγ亚基的表达和纯化,用于结构和动力学研究 作者:Wenjie Zhao, Xudong Wang and Libin Ye日期:02/20/2021,浏览量:900,Q&A: 0

In the last several years, as evidence of a surged number of GPCR-G complex structures, the expressions of GPCRs and G proteins for structural biology have achieved tremendous successes, mostly in insect and mammalian cell systems, resulting in more than 370 structures of over 70 GPCRs have been resolved. However, the challenge remains,

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Extraction and Quantification of Sphingolipids from Hemiptera Insects by Ultra-Performance Liquid Chromatography Coupled to Tandem Mass Spectrometry 超高效液相色谱-串联质谱联用法提取和定量半翅目昆虫鞘脂质 作者:Ni Wang, Xiaoxiao Shi, Chao Zhang, Wenwu Zhou and Zeng-Rong Zhu日期:02/20/2021,浏览量:527,Q&A: 0

Sphingolipids are major structural components of endomembranes and have also been described as an intracellular second messenger involved in various biological functions in all eukaryotes and a few prokaryotes. Ceramides (Cer), the central molecules of sphingolipids, have been depicted in cell growth arrest, cell differentiation, and apoptosis.

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Tracing Nitrogen Metabolism in Mouse Tissues with Gas Chromatography-Mass Spectrometry 用气相色谱-质谱法追踪小鼠组织中的氮代谢 作者:Rong Xu, Yekai Wang and Jianhai Du日期:02/20/2021,浏览量:592,Q&A: 0

Nitrogen-containing metabolites including ammonia, amino acids, and nucleotides, are essential for cell metabolism, growth, and neural transmission. Nitrogen metabolism is tightly coordinated with carbon metabolism in the breakdown and biosynthesis of amino acids and nucleotides. Both nuclear magnetic resonance spectroscopy and mass spectrometry

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生物物理学

Live Cell FRET Analysis of the Conformational Changes of Human P-glycoprotein 人P-糖蛋白构象变化的活细胞FRET分析 作者:Ryota Futamata, Noriyuki Kioka and Kazumitsu Ueda日期:02/20/2021,浏览量:697,Q&A: 0

The molecular mechanisms of P-glycoprotein (P-gp; also known as MDR1 or ABCB1) have been mainly investigated using artificial membranes such as lipid-detergent mixed micelles, artificial lipid bilayers, and membrane vesicles derived from cultured cells. Although these in vitro experiments help illustrate details about the molecular mechanisms of

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细胞生物学

Bioorthogonal Labeling and Chemoselective Functionalization of Lung Extracellular Matrix 肺细胞外基质的生物正交标记和化学选择性功能化 作者:Zihan Ling, Yunhui Xing, Emily Reinoso Jacome, Shierly W. Fok and Xi Ren日期:02/20/2021,浏览量:559,Q&A: 0

Decellularized extracellular matrix (ECM) biomaterials derived from native tissues and organs are widely used for tissue engineering and wound repair. To boost their regenerative potential, ECM biomaterials can be functionalized via the immobilization of bioactive molecules. To enable ECM functionalization in a chemoselective manner, we have

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发育生物学

Molecular and Phenotypic Characterization Following RNAi Mediated Knockdown in Drosophila RNAi介导的基因敲除后果蝇的分子和表型特征 作者:Saurabh Jayesh Kumar Mehta, Pradyumna A. Joshi and Ram Kumar Mishra日期:02/20/2021,浏览量:607,Q&A: 0

Loss of function studies shed significant light on the involvement of a gene or gene product in different cellular processes. Short hairpin RNA (shRNA) mediated RNA interference (RNAi) is a classical yet straightforward technique frequently used to knock down a gene for assessing its function. Similar perturbations in gene expression can be

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免疫学

In vitro Measurement of Membrane Attack Complex in RPE Cells 视网膜色素上皮细胞膜攻击复合物的体外测定 作者:Kelly Mulfaul and Sarah L. Doyle日期:02/20/2021,浏览量:539,Q&A: 0

Initiation of the complement system results in the formation of a multiprotein pore termed the membrane attack complex (MAC, C5b-C9). MAC pores accumulate on a cell surface and can result in cell lysis. The retinal pigment epithelium (RPE) is a single monolayer of pigmented epithelial cells located at the posterior poll of the eye that forms the

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An Imaging Flow Cytometry Method to Measure Citrullination of H4 Histone as a Read-out for Neutrophil Extracellular Traps Formation 一种成像流式细胞术测定组蛋白H4瓜氨酸化的方法,作为中性粒细胞胞外陷阱形成的信号通路 作者:Emilia A. Barbu, Venina M. Dominical, Laurel Mendelsohn and Swee Lay Thein日期:02/20/2021,浏览量:537,Q&A: 0

The formation of neutrophil extracellular traps (NETs) is thought to play a critical role in infections and propagating sterile inflammation. Histone citrullination is an essential and early step in NETs formation, detectable prior to the formation of the hallmark extracellular DNA-scaffolded strands. In addition to the classical microscopy

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微生物学

Rapid Genome Engineering of Pseudomonas Assisted by Fluorescent Markers and Tractable Curing of Plasmids 受荧光标记和易于固化质粒影响的假单胞菌快速基因组工程 作者:Daniel C. Volke, Nicolas T. Wirth and Pablo I. Nikel日期:02/20/2021,浏览量:1152,Q&A: 0

Precise genome engineering has become a commonplace technique for metabolic engineering. Also, insertion, deletion and alteration of genes and other functional DNA sequences are essential for understanding and engineering cells. Several techniques have been developed to this end (e.g., CRISPR/Cas-assisted methods, homologous recombination, or λ

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分子生物学

RI-SEC-seq: Comprehensive Profiling of Nonvesicular Extracellular RNAs with Different Stabilities RI-SEC-seq:具有不同稳定性的非泡性细胞外RNA的综合分析 作者:Juan Pablo Tosar, Fabiana Gámbaro, Mauricio Castellano and Alfonso Cayota日期:02/20/2021,浏览量:700,Q&A: 0

Exosomes and other extracellular vesicles (EVs) are considered the main vehicles transporting RNAs in extracellular samples, including human bodily fluids. However, a major proportion of extracellular RNAs (exRNAs) do not copurify with EVs and remain in ultracentrifugation supernatants of cell-conditioned medium or blood serum. We have observed

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植物科学

Phytophthora infestans (Late blight) Infection Assay in a Detached Leaf of Potato 在离体叶片中的马铃薯疫霉(晚疫病)感染实验 作者:Hari S. Karki and Dennis A. Halterman日期:02/20/2021,浏览量:600,Q&A: 0

Phytophthora infestans is a hemibiotroph oomycete that primarily infects potato and tomato. It infects stems, leaves, and tubers and fruits of potato and tomato. High throughput and reproducible infection assays are prerequisites to find sources of resistance in any crop. In this protocol, we describe a detached leaf assay (DLA) for conducting the

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干细胞

Generation of the Compression-induced Dedifferentiated Adipocytes (CiDAs) Using Hypertonic Medium 使用高渗培养基压缩诱导的去分化脂肪细胞(CiDAs)的产生 作者:Yiwei Li, Angelo S. Mao, Bo Ri Seo, Xing Zhao, Satish Kumar Gupta, Maorong Chen, Yu Long Han, Ting-Yu Shih, David J. Mooney and Ming Guo日期:02/20/2021,浏览量:376,Q&A: 0

Current methods to obtain mesenchymal stem cells (MSCs) involve sampling, culturing, and expanding of primary MSCs from adipose, bone marrow, and umbilical cord tissues. However, the drawbacks are the limited numbers of total cells in MSC pools, and their decaying stemness during in vitro expansion. As an alternative resource, recent ceiling

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系统生物学

EmPC-seq: Accurate RNA-sequencing and Bioinformatics Platform to Map RNA Polymerases and Remove Background Error EmPC-seq: 使用精确的RNA测序和生物信息学平台绘制RNA聚合酶并消除背景错误 作者:Yuqing Wang, Tin Hang Chong, Ilona Christy Unarta, Xinzhou Xu, Gianmarco D. Suarez, Jiguang Wang, John T. Lis, Xuhui Huang and Peter Pak-Hang Cheung日期:02/20/2021,浏览量:400,Q&A: 0

Transcription errors can substantially affect metabolic processes in organisms by altering the epigenome and causing misincorporations in mRNA, which is translated into aberrant mutant proteins. Moreover, within eukaryotic genomes there are specific Transcription Error-Enriched genomic Loci (TEELs) which are transcribed by RNA polymerases with

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