Request a Protocol - Bio-protocol

Request a Protocol

If you would like a more detailed, step-by-step version of a protocol derived from a research article, use the Request a Protocol (RaP) feature, powered by Bio-protocol.

As part of our efforts to make science more reproducible, Bio-protocol has collaborated with partners such as Science/AAAS and eLife to make it easier to view and request detailed protocols directly from their published articles.

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“AAAS launches collaboration with Bio-protocol, in support of scientific reproducibility”
AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE

NEWS RELEASE 13-AUG-2019

eLife Latest: Request detailed protocols easily via Bio-protocol integration
Our new reproducibility-focused integration with Bio-protocol lets you request a detailed protocol from any article on elife.

Inside eLife. 21-Aug-2019

Please log in to fill out the request form, and we will contact the author(s) on your behalf. If the author agrees to share the protocol details you are requesting, we will let you know via email. Please do not request that authors share details that are already available in their research article or supplementary materials.

Research Article

Cystatin from Filarial Parasites Suppress the Clinical Symptoms and Pathology of Experimentally Induced Colitis in Mice by Inducing T-Regulatory Cells, B1-Cells, and Alternatively Activated Macrophages
Ramaswamy Kalyanasundaram et al.

DOI: 10.3390/biomedicines7040085

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Choose one protocol to request:

4.1. Expression and Purification of rBmaCys

4.2. Animals

4.8. Statistical Analysis

4.7. Flow Cytometric Analysis of Treg and B-1 Cells

4.6. Levels of Nitric Oxide in the Culture Supernatants of Peritoneal Macrophages

4.5. qPCR Analysis to Determine Relative Cytokine mRNA Expression in the Colon Tissue and in Peritoneal Macrophages

4.4. Evaluation of Colitis in the Mice Treated with rBmaCys

4.3. DSS-Induced Colitis Model

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