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Request a Protocol

If you would like a more detailed, step-by-step version of a protocol derived from a research article, use the Request a Protocol (RaP) feature, powered by Bio-protocol.

As part of our efforts to make science more reproducible, Bio-protocol has collaborated with partners such as Science/AAAS and eLife to make it easier to view and request detailed protocols directly from their published articles.

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“AAAS launches collaboration with Bio-protocol, in support of scientific reproducibility”
AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE

NEWS RELEASE 13-AUG-2019

eLife Latest: Request detailed protocols easily via Bio-protocol integration
Our new reproducibility-focused integration with Bio-protocol lets you request a detailed protocol from any article on elife.

Inside eLife. 21-Aug-2019

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Research Article

Thrombopoietin Promotes Cell Proliferation and Attenuates Apoptosis of Aplastic Anemia Serum-Treated 32D Cells via Activating STAT3/STAT5 Signaling Pathway and Modulating Apoptosis-Related Mediators
Juan Qian et al.

DOI: 10.1177/0963689720980367

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Choose one protocol to request:

Cell Line and Cell Culture

Lentivirus Infection and Serum and TPO Treatment

Analysis of 32D Cell Number by Trypan Blue Counting

Determination of 32D Cell Proliferation Using Cell Counting Kit-8 (CCK-8) Assay

Flow Cytometry to Detect Apoptosis of 32D Cells

RNA Extraction and Quantitative Real-Time PCR (qRT-PCR)

Western Blot Assay

Statistical Analysis

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