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Experimental Pipeline for SNP and SSR Discovery and Genotyping Analysis of Mango (Mangifera indica L.)

Authors: Michal Sharabi-Schwager, Mor Rubinstein, Mazal Ish shalom, Ravit Eshed, Ada Rozen, Amir Sherman, Yuval Cohen and Ron Ophir, date: 08/20/2016, view: 9358, Q&A: 0

[Abstract] Establishing a reservoir of polymorphic markers is an important key for marker-assisted breeding. Many crops are still lack of such genomic infrastructure. Single nucleotide polymorphisms (SNPs) and simple sequence repeats (SSRs) are useful as markers because they are widespread over the genome and many technologies were developed for high ...

EST-SSR Analysis and Cross-species Transferability Study in Lavandula

Authors: Ayelign M. Adal, Zerihun A. Demissie and Soheil S. Mahmoud, date: 08/05/2016, view: 8716, Q&A: 0

[Abstract] The genus Lavandula comprises of several economically important lavender species that are mainly cultivated worldwide for essential oil production. Identification of lavender species and their cultivars has been a huge bottleneck in lavender industries due to lack of appropriate identification mechanisms. Recent advances in ...

Identification of Natural Hybrids by SSR Markers in Mussaenda

Authors: Zhonglai Luo, Tingting Duan, Shuai Yuan, Shi Chen, Xiufeng Bai and Dianxiang Zhang, date: 07/05/2016, view: 8194, Q&A: 0

[Abstract] Detection of natural hybrids is of great significance for plant taxonomy, reproductive biology, and population genetic studies. Compared with methods depending on morphological characters, molecular markers provide reliable and much more accurate results. This protocol describes approaches employing microsatellite (SSR) markers to identify ...

Pyrosequencing Approach for SNP Genotyping in Plants Using a M13 Biotinylated Primer

Authors: Cristina Silvar, Dragan Perovic, Anna M Casas, Ernesto Igartua and Frank Ordon, date: 05/20/2015, view: 9213, Q&A: 0

[Abstract] Single Nucleotide Polymorphisms (SNPs), which constitute single base-pair variations in the DNA sequence, are the most abundant molecular markers in plant and animal genomes. They are becoming the markers of choice for genotyping in all fields of molecular biology, as they are easily prone to automation and high throughput, for example through ...

Fluorescence-based CAPS Multiplex Genotyping on Capillary Electrophoresis Systems

Authors: Jelena Perovic, Cristina Silvar, Dragan Perovic, Nils Stein and Frank Ordon, date: 05/20/2015, view: 8665, Q&A: 0

[Abstract] Recent advances in next-generation sequencing techniques allow the detection of a large number of SNPs and their use in a high throughput manner. However, Cleaved Amplified Polymorphic Sequences (CAPSs) still play a significant role as complement to other high throughput methods for SNP genotyping. Therefore, new methods focusing on the ...

Detection of Transposable Element Insertion Site Polymorphisms by Sequence-Specific Amplification Polymorphism (SSAP)

Authors: Véronique Sarilar, Paulina Martinez Palacios and Karine Alix, date: 03/05/2014, view: 10072, Q&A: 0

[Abstract] Transposable elements represent a major part of any eukaryotic genomes. Notably in plants they can account for more than 80% of the whole genomic sequence (such as in maize). Due to their mobility across the genome, they can act as mutagens but can also be considered as an important source of genetic diversity. It has been shown that they may be ...

High Resolution Detection of Genetic Changes Associated with Transposons

Authors: Beery Yaakov and Khalil Kashkush, date: 06/05/2013, view: 9031, Q&A: 0

[Abstract] Transposable elements (TEs) are repetitive sequences, capable of inducing genetic mutations through their transpositional activity, or by non-homologous or illegitimate recombination. Because of their similarity and often high copy numbers, examining the effects of mutations caused by TEs in different samples (tissues, individuals, species, etc.) ...

High-throughput Method for Determination of Seed Paternity by Microsatellite Markers

Authors: Samik Bhattacharya and Ian T. Baldwin, date: 04/20/2013, view: 12236, Q&A: 0

[Abstract] In this protocol, determination of seed paternity by microsatellite markers in Nicotiana attenuata is described. However, this does not include a protocol for the novel marker selection/identification, but rather exploits the markers generated for a closely related species N. tabacum (Bindler et al., 2007). This is a ...

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