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Analysis of
N
-acetylmuramic acid-6-phosphate (MurNAc-6P) Accumulation by HPLC-MS
Authors:
Marina Borisova
and
Christoph Mayer
,
date:
08/05/2017,
view:
7506,
Q&A:
0
[Abstract] We describe here in detail a high-performance liquid chromatography-mass spectrometry (HPLC-MS)-based method to determine
N
-acetylmuramic acid-6-phosphate (MurNAc-6P) in bacterial cell extracts. The method can be applied to both Gram-negative and Gram-positive bacteria, and as an example we use
Escherichia coli
cells in this ...
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