Protocols in Current Issue
    Multiplex Gene Editing via CRISPR/Cas9 System in Sheep
    Authors:  Yiyuan Niu, Yi Ding, Xiaolong Wang and Yulin Chen, date: 07/05/2017, view: 9480, Q&A: 0
    [Abstract] Sheep is a major large animal model for studying development and disease in biomedical research. We utilized CRISPR/Cas9 system successfully to modify multiple genes in sheep. Here we provide a detailed protocol for one-cell-stage embryo manipulation by co-injecting Cas9 mRNA and RNA guides targeting three genes (MSTN, ASIP, and ...

    We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.