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Multiplex Gene Editing via CRISPR/Cas9 System in Sheep
Authors:
Yiyuan Niu
,
Yi Ding
,
Xiaolong Wang
and
Yulin Chen
,
date:
07/05/2017,
view:
9480,
Q&A:
0
[Abstract] Sheep is a major large animal model for studying development and disease in biomedical research. We utilized CRISPR/Cas9 system successfully to modify multiple genes in sheep. Here we provide a detailed protocol for one-cell-stage embryo manipulation by co-injecting Cas9 mRNA and RNA guides targeting three genes (
MSTN
,
ASIP
, and
...
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