Prokaryotes

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    Protocols in Current Issue
    Nitrite Reduction Assay for Whole Pseudomonas Cells
    Authors:  Michael P. Thorgersen and Michael W. Adams, date: 05/20/2016, view: 10640, Q&A: 1
    [Abstract] The second step of the dissimilatory denitrification pathway in which nitrite (NO2-) is converted to nitric oxide (NO) is catalyzed by the enzyme nitrite reductase. Two distinct enzymes are found in nature that catalyze this reaction, and they contain different metal sites, either iron (Fe), in the form of heme, or copper ...
    Identification and Characterization of Bacterial Chemoreceptors Using Quantitative Capillary and Gradient Plate Chemotaxis Assays
    [Abstract] Bacterial chemotaxis is a motility-based response that biases cell movement toward beneficial molecules, called attractants, and away from harmful molecules, also known as repellents. Since the species of the genus Pseudomonas are characterized by a metabolic versatility, these bacteria have developed chemotactic behaviors towards a wide range of ...
    Quantification of Respiratory Activity in Biofilms
    Authors:  Cláudia N. H. Marques and Scott A. Craver, date: 09/20/2015, view: 9332, Q&A: 1
    [Abstract] Bacteria live mostly as biofilms, not as planktonic cell populations. Bacterial cells living as biofilms are known to be in different physiological status. Persister cells are one of such physiological conditions and they are recognized as to be a stochastically produced sub-population of non-growing bacterial cells. The following protocol ...
    Isolation of Persister Cells from Biofilm and Planktonic Populations of Pseudomonas aeruginosa
    Author:  Cláudia N. H. Marques, date: 09/20/2015, view: 11231, Q&A: 0
    [Abstract] Persister cells are a stochastically produced sub-population of non-growing bacterial cells. Recently these cells have been more widely studied due to the recognition that they are tolerant to antimicrobials and thus, play a major role in the resilience of bacterial populations to antimicrobials, particularly in chronic biofilm infections. The ...
    Cell Fractionation of Pseudomonas aeruginosa
    Authors:  Esteban Paredes-Osses and Kim R. Hardie, date: 10/05/2013, view: 11819, Q&A: 1
    [Abstract] Pseudomonas aeruginosa is a Gram negative bacterium. Separating the cell envelope compartments enables proteins to be localized to confirm where in the cell they function. Cell fractionation can also provide a first step in a protein purification strategy (Williams et al., 1998). This protocol has been designed to obtain the ...



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