Mammalia

Categories

    Protocols in Current Issue
    Measurement of Bone Metastatic Tumor Growth by a Tibial Tumorigenesis Assay
    Authors:  Baotong Zhang, Xin Li, Wei-Ping Qian, Daqing Wu and Jin-Tang Dong, date: 11/20/2021, view: 1603, Q&A: 0
    [Abstract]

    Bone metastasis is a frequent and lethal complication of many cancer types (i.e., prostate cancer, breast cancer, and multiple myeloma), and a cure for bone metastasis remains elusive. To recapitulate the process of bone metastasis and understand how cancer cells metastasize to bone, intracardiac injection and intracaudal arterial animal models

    ...
    Multi-color Flow Cytometry for Comprehensive Analysis of the Tumor Immune Infiltrate in a Murine Model of Breast Cancer
    Authors:  Ana S. Almeida, Miriam R. Fein and Mikala Egeblad, date: 06/05/2021, view: 2950, Q&A: 0
    [Abstract]

    Flow cytometry is a popular laser-based technology that allows the phenotypic and functional characterization of individual cells in a high-throughput manner. Here, we describe a detailed procedure for preparing a single-cell suspension from mammary tumors of the mouse mammary tumor virus-polyoma middle T (MMTV-PyMT) and analyzing these cells by

    ...
    In vivo Mouse Mammary Gland Formation
    Authors:  Heng Sun, Xin Zhang, Un In Chan, Sek Man Su, Sen Guo, Xiaoling Xu and Chuxia Deng, date: 07/05/2020, view: 2421, Q&A: 0
    [Abstract] For years, the mammary gland serves as a perfect example to study the self-renew and differentiation of adult stem cells, and the regulatory mechanisms of these processes as well. To assess the function of given genes and/or other factors on stemness of mammary cells, several In vitro assays were developed, such as mammospheres formation ...
    Preparation of Single Epithelial Cells Suspension from Mouse Mammary Glands
    Authors:  Heng Sun, Xiaoling Xu and Chuxia Deng, date: 02/20/2020, view: 3376, Q&A: 0
    [Abstract] Single cell RNA sequencing is a very powerful means for cellular heterogeneous studies and so becoming wildly utilized nowadays. To guarantee the success of such analysis, it is very important, though sometimes difficult, to obtain single cells suspension with high quality, especially from the primary solid organs like mammary glands. Digestion of ...
    Isolation of Primary Breast Cancer Cells from HER2 Transgenic Mice
    Authors:  Shou Liu and Hexin Chen, date: 10/05/2016, view: 12569, Q&A: 1
    [Abstract] HER2 is a tyrosine kinase receptor, which is overexpressed in about 30% of breast cancer patients. Its overexpression leads to mammary tumorigenesis and increased invasion and metastasis (Slamon et al., 1987). HER2 transgenic mouse (FVB/N-MMTVneu mouse) is a well-established model of mammary tumor in human (Fantozzi and Christofori, ...



    We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.