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    Protocols in Current Issue
    Histochemical Staining of Suberin in Plant Roots
    Authors:  Peter Marhavý and Shahid Siddique, date: 02/05/2021, view: 3701, Q&A: 1
    [Abstract]

    Histological stains are useful tools for characterizing cell shape, arrangement and the material they are made from. Stains can be used individually or simultaneously to mark different cell structures or polymers within the same cells, and to visualize them in different colors. Histological stains can be combined with genetically-encoded

    ...
    A Protocol for Flavonols, Kaempferol and Quercetin, Staining in Plant Root Tips
    Author:  Nguyen Hoai Nguyen, date: 10/05/2020, view: 2722, Q&A: 0
    [Abstract] Flavonols are a subclass of flavonoids of the group of plant secondary metabolites. In planta, flavonols play various functions such as antioxidant and natural regulator of auxin polar transport. Many lines of evidence have shown that flavonols also contribute to human health in anti-oxidation, anti-inflammation, and even prevention some ...
    Ensifer-mediated Arabidopsis thaliana Root Transformation (E-ART): A Protocol to Analyse the Factors that Support Ensifer-mediated Transformation (EMT) of Plant Cells
    Authors:  Dheeraj Singh Rathore, Fiona M. Doohan and Ewen Mullins, date: 10/05/2017, view: 9315, Q&A: 0
    [Abstract] Ensifer adhaerens OV14, a soil borne alpha-proteobacteria of the Rhizobiaceae family, fortifies the novel plant transformation technology platform termed ‘Ensifer-mediated transformation’ (EMT). EMT can stably transform both monocot and dicot species, and the host range of EMT is continuously expanding across a diverse range of ...
    Measurement of 33P-PO4 Absorption Capacity and Root-to-leaf Transfer in Arabidopsis
    Authors:  Marie-Christine Thibaud and Elena Marin, date: 03/05/2016, view: 6423, Q&A: 0
    [Abstract] This method allows quantification of phosphate absorption capacity by Arabidopsis roots using very simple equipment, and can be scaled up or down.
    Measurement of 33P-PO4 Absorption Kinetic Constants in Arabidopsis
    Authors:  Elena Marin and Marie-Christine Thibaud, date: 03/05/2016, view: 6455, Q&A: 0
    [Abstract] Based on the Michaelis-Menten kinetics model (Hofstee, 1952), this method allows calculation of the kinetic parameters (Vmax, Km) of phosphate uptake by Arabidopsis roots. This method is based on the quantification of phosphate uptake by Arabidopsis roots as described in Thibaud and Marin (2016), except that ...
    High-throughput Quantification of Ammonium Content in Arabidopsis
    Authors:  Izargi Vega-Mas, Asier Sarasketa and Daniel Marino, date: 08/20/2015, view: 9796, Q&A: 0
    [Abstract] This protocol is a simple colorimetric assay for internal ammonium quantification in aqueous extracts from plant tissues. The method is based on the phenol hypochlorite assay (Berthelot reaction):

    NH4+ + hypochlorite + OH- + phenol → indophenol

    The oxidation of indophenol caused by phenol ...
    Localisation and Quantification of Reactive Oxygen Species and Nitric Oxide in Arabidopsis Roots in Response to Fungal Infection
    Authors:  Kapuganti J. Gupta, Yariv Brotman and Luis A. J. Mur, date: 10/05/2014, view: 10921, Q&A: 0
    [Abstract] Nitric oxide and reactive oxygen species have emerged as important signalling molecules in plants. The half-lives of NO and ROS are very short therefore rapid and precise measurements are required for the understanding biological roles of these redox active species. Various organelles and compartments generate NO and ROS thus it is important to ...



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