Protocols in Current Issue
    Microscopic Observation, Three-dimensional Reconstruction, and Volume Measurements of Sperm Nuclei
    Authors:  Yukinosuke Ohnishi and Takashi Okamoto, date: 04/05/2015, view: 7810, Q&A: 0
    [Abstract] Karyogamy, a migration of the sperm nucleus toward the egg nucleus and their subsequent nuclear fusion, is an important biological event for initiating zygote formation toward early embryogenesis in angiosperms. However, how the male nucleus approaches and fuses with the female nucleus still remains unclear. Recently, time-lapse measurement of ...
    Rice Meiotic Chromosome Spread Preparation of Pollen Mother Cells
    Authors:  Xingwang Li and Changyin Wu, date: 07/20/2014, view: 9946, Q&A: 0
    [Abstract] In this protocol, we describe a simple and efficient method for meiotic chromosome spread preparation in rice pollen mother cells. Meiotic chromosome preparation by spreading itself is an important technique for plant cytogenetics (Higgins et al., 2004; Chelysheva et al., 2012; Wang et al., 2009); furthermore, it is a ...

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