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    Protocols in Current Issue
    Fluorescence Microscopy Analysis of Drug Effect on Autophagosome Formation
    Authors:  Metodi Stankov, Diana Panayotova-Dimitrova, Martin Leverkus and Georg Behrens, date: 04/05/2014, view: 10195, Q&A: 0
    [Abstract] The autophagy protein, LC3 represents a reliable characteristic marker for autophagosomal structures. The initial LC3 is processed by the cysteine protease autophagy-related gene 4 (Atg4) at its C terminus in order to create LC3-I generally localized in the cytoplasm. Afterwards LC3-I is conjugated with phosphatidylethanolamine (PE) to become ...



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