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Fluorescence Microscopy Analysis of Drug Effect on Autophagosome Formation
Authors:
Metodi Stankov
,
Diana Panayotova-Dimitrova
,
Martin Leverkus
and
Georg Behrens
,
date:
04/05/2014,
view:
10195,
Q&A:
0
[Abstract] The autophagy protein, LC3 represents a reliable characteristic marker for autophagosomal structures. The initial LC3 is processed by the cysteine protease autophagy-related gene 4 (Atg4) at its C terminus in order to create LC3-I generally localized in the cytoplasm. Afterwards LC3-I is conjugated with phosphatidylethanolamine (PE) to become ...
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