Protocols in Current Issue
    Analysis of Isotopically-labeled Monogalactosyldiacylglycerol Molecular Species from [14C]Acetate-Labeled Tobacco Leaves
    Authors:  Hari Kiran Kotapati and Philip D. Bates, date: 12/20/2020, view: 1485, Q&A: 0

    Plant lipid metabolism is a dynamic network where synthesis of essential membrane lipids overlaps with synthesis of valuable storage lipids (e.g., vegetable oils). Monogalactosyldiacylglycerol (MGDG) is a key component of the chloroplast membrane system required for photosynthesis and is produced by multiple pathways within the lipid metabolic

    Application of Silicone Tubing for Robust, Simple, High-throughput, and Time-resolved Analysis of Plant Volatiles in Field Experiments
    Authors:  Mario Kallenbach, Daniel Veit, Elisabeth J. Eilers and Meredith C. Schuman, date: 02/05/2015, view: 12749, Q&A: 1
    [Abstract] Plant volatiles (PVs) mediate manifold interactions between plants and their biotic and abiotic environments (Dicke and Baldwin, 2010; Holopainen and Gershenzon, 2010). An understanding of the physiological and ecological functions of PVs must therefore be based on measurements of PV emissions under natural conditions. Yet sampling PVs in natural ...
    Detection and Measurement of ROS in Tobacco Leaves
    Authors:  Shunhua Ding and Congming Lu, date: 03/05/2013, view: 12008, Q&A: 1
    [Abstract] Leaf metabolism produces hydrogen peroxide (H2O2) at high rates, high level H2O2 accumulation can cause oxidative stress. This protocol describes a method for determining H2O2 concentration in tobacco leaves. In this method all extractions were performed with HClO4, ...

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