Protocols in Current Issue
    Preference Test of Plutella xylostella Larvae upon DMNT Treatment
    Authors:  Chen Chen, Zhen Tao, Chuanhong Wang, Tengyue Wang, Hongyi Chen, Taoshan Jiang and Peijin Li, date: 11/05/2021, view: 898, Q&A: 0

    We describe a method to test the preference of insects in response to (3E)-4,8-dimethyl-1,3,7-nonatriene (DMNT). We use a device that includes a horizontal glass tube, two grooves (with activated carbon), air flow, rubber stoppers/tubes, transparent glass containers (optional), and a holder for the glass tube (optional). Equal amounts of activated

    Quantification of Salicylic Acid (SA) and SA-glucosides in Arabidopsis thaliana
    [Abstract] Homeostasis between the cytoplasmic plant hormone salicylic acid (SA) and its’ inactive, vacuolar storage forms, SA-2-O-β-D-glucoside (SAG) and SA-β-D-Glucose Ester (SGE), regulates the fine-tuning of defense responses to biotrophic pathogens in Arabidopsis thaliana. This protocol describes a simplified, optimized procedure to ...
    Root Aliphatic Suberin Analysis Using Non-extraction or Solvent-extraction Methods
    Authors:  Camille Delude, Sollapura J. Vishwanath, Owen Rowland and Frédéric Domergue, date: 06/20/2017, view: 8566, Q&A: 1
    [Abstract] Here we describe both non-extraction and solvent-extraction methods for root aliphatic suberin analysis. The non-extraction method is fast as roots are directly depolymerized using acidic transmethylation. However, suberin aliphatic components are isolated together with all the other acyl chains making up the lipids (e.g., membranes) ...
    Simple Spectroscopic Determination of Nitrate, Nitrite, and Ammonium in Arabidopsis thaliana
    Authors:  Takushi Hachiya and Yuki Okamoto, date: 05/20/2017, view: 17442, Q&A: 2
    [Abstract] Plants use nitrate, nitrite, and ammonium as inorganic nitrogen (N) sources. These N compounds are included in plant tissues at various concentrations depending on the balance between their uptake and assimilation. Thus, the contents of nitrate, nitrite, and ammonium are physiological indicators of plant N economy. Here, we describe a protocol for ...
    Nitrate Assay for Plant Tissues
    Authors:  Lufei Zhao and Yong Wang, date: 01/20/2017, view: 15290, Q&A: 4
    [Abstract] Nitrogen is an essential macronutrient for plant growth and nitrate content in plants can reflect the nitrogen supply of soil. Here, we provide the salicylic acid method to evaluate the nitrate content in plant tissues. The method is reliable and stable, thus it can be a good choice for measurement of nitrate in plant tissues.
    Cell Wall-bound p-Coumaric and Ferulic Acid Analysis
    Author:  Nickolas Anderson, date: 10/05/2016, view: 6757, Q&A: 0
    [Abstract] Hydroxycinnamic acids, such as p-coumaric acid and ferulic acid, are a major class of compounds derived from the phenylpropanoid pathway. These compounds are widely conserved in plants and primarily accumulate in the secondary cell wall. They serve as important structural components that contribute to the overall strength and rigidity of ...
    Measurement of Uptake and Root-to-Shoot Distribution of Sulfate in Arabidopsis Seedlings
    Authors:  Naoko Yoshimoto, Tatsuhiko Kataoka, Akiko Maruyama-Nakashita and Hideki Takahashi, date: 01/05/2016, view: 8286, Q&A: 0
    [Abstract] Sulfur is an essential macronutrient required for growth and development of plants. Plants take up sulfate from the soil environment through the function of plasma membrane-bound sulfate transporters expressed at the root surface cell layers. Plants then utilize the incorporated sulfate as the main sulfur source to synthesize sulfur-containing ...
    Arabidopsis Metabolome Analysis Using Infusion ESI FT-ICR/MS
    Authors:  Reiko Motohashi, Masakazu Satou, Fumiyoshi Myouga, Akira Oikawa and Daisaku Ohta, date: 05/05/2015, view: 7499, Q&A: 0
    [Abstract] We made the method for Arabidopsis metabolome analysis based on direct-infusion Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR/MS) (IonSpec). This method was sufficiently applied to metabolic phenotyping of Arabidopsis. This method is simple in that after homogenizing samples, powdered samples are dissolved ...
    Lignin Extraction and Quantification, a Tool to Monitor Defense Reaction at the Plant Cell Wall Level
    Authors:  Sebastian T. Schenk and Adam Schikora, date: 03/20/2015, view: 13910, Q&A: 1
    [Abstract] Lignin is a complex polymer of phenolic compounds (monolignins), which contributes to the rigidity of the plant cell wall. Lignification is essential for plant development, however it is also one of the mechanisms of plant defense. Accumulation of lignin and the polymerization of monolignins at sides of pathogen attack protect the cell wall ...
    Endogenous ABA Extraction and Measurement from Arabidopsis Leaves
    Authors:  Ning Liu, Yong Ding, Michael Fromm and Zoya Avramova, date: 10/05/2014, view: 11047, Q&A: 0
    [Abstract] The endogenous messenger, the phytohormone abscisic acid (ABA) plays a major role in plant’s adaption to drought, salinity, cold and other abiotic stresses. In addition to abiotic stress signaling, ABA is involved also in developmental regulation and in responses to diverse biotic stresses. Dehydration stress results in a strong increase in ...

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