Protocols in Current Issue
    Histone modification ChIP-seq on Arabidopsis thaliana Plantlets
    Authors:  Juan S. Ramirez-Prado, David Latrasse and Moussa Benhamed, date: 11/05/2021, view: 1663, Q&A: 1

    Characterizing the molecular mechanisms regulating gene expression is crucial for understanding the regulatory processes underlying physiological responses to environmental and developmental signals in eukaryotes. The covalent modification of histones contributes to the compaction levels of chromatin, as well as the recruitment of the

    Determination of Luciferase Activity in Arabidopsis seedling
    Authors:  Mohan TC, Gabriel Castrillo and Antonio Leyva, date: 06/20/2014, view: 11533, Q&A: 0
    [Abstract] There are different direct and indirect methods available to study gene expression in plant systems. In this protocol we describe a modified expression assay using transgenic plants expressing the luciferase gene under the control of phosphate transporter PHT1;1 promoter. This assay was originally optimized for analyzing the repression of PHT1;1 ...
    DNA Damage Sensitivity Assays with Arabidopsis Seedlings
    Authors:  Marisa Rosa and Ortrun Mittelsten Scheid, date: 04/05/2014, view: 9727, Q&A: 0
    [Abstract] We describe fast and reproducible sensitivity assays to quantify the response of Arabidopsis seedlings of different genotypes to a wide range of DNA damaging agents. We apply (1) γ-irradiation, which produces DNA breaks, (2) bleocin, a radiomimetic drug, (3) mitomycin C, a DNA intrastrand cross-linker, (4) hydroxyurea, an inhibitor of DNA ...
    Measuring Homologous Recombination Frequency in Arabidopsis Seedlings
    Authors:  Marisa Rosa and Ortrun Mittelsten Scheid, date: 04/05/2014, view: 11422, Q&A: 0
    [Abstract] Somatic homologous recombination (SHR) is a major pathway of DNA double-strand break (DSB) repair, in which intact homologous regions are used as a template for the removal of lesions. Its frequency in plants is generally low, as most DSB are removed by non-homologous mechanisms in higher eukaryotes. Nevertheless, SHR frequency has been shown to ...
    Micrococcal Nuclease (MNase) Assay of Arabidopsis thaliana Nuclei
    Authors:  Laia Armengot and Jordi Moreno-Romero, date: 04/05/2013, view: 22078, Q&A: 3
    [Abstract] Micrococcal nuclease (MNase) is able to produce double-strand breaks within nucleosome linker regions. The efficiency of MNase digestion depends on the degree of chromatin compaction, being more easily digested the regions of less compacted chromatin. The MNase protocol described here can be used to asses changes in the chromatin structure of ...

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