Prokaryotes

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    Protocols in Current Issue
    EMSA Analysis of DNA Binding By Rgg Proteins
    Authors:  Breah LaSarre and Michael J. Federle, date: 08/05/2013, view: 12514, Q&A: 0
    [Abstract] In bacteria, interaction of various proteins with DNA is essential for the regulation of specific target gene expression. Electrophoretic mobility shift assay (EMSA) is an in vitro approach allowing for the visualization of these protein-DNA interactions. Rgg proteins comprise a family of transcriptional regulators widespread among the ...



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