Prokaryotes

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    Protocols in Current Issue
    Construction and Screening of a Transposon Insertion Library of Yersinia enterocolitica (YeO3-R1)
    Authors:  Maria Pajunen, Elise Pinta and Mikael Skurnik, date: 08/05/2012, view: 13607, Q&A: 0
    [Abstract] The Mu-transposon system is one of the best characterized transposition systems. Under minimal in vitro set-up, Mu transposition requires only a simple reaction buffer, MuA transposase protein, mini-Mu transposon DNA (donor) and target DNA. The reaction proceeds via initial assembly of the transposition complex that directs transposon ...



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