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Construction and Screening of a Transposon Insertion Library of
Yersinia enterocolitica
(YeO3-R1)
Authors:
Maria Pajunen
,
Elise Pinta
and
Mikael Skurnik
,
date:
08/05/2012,
view:
13607,
Q&A:
0
[Abstract] The Mu-transposon system is one of the best characterized transposition systems. Under minimal
in vitro
set-up, Mu transposition requires only a simple reaction buffer, MuA transposase protein, mini-Mu transposon DNA (donor) and target DNA. The reaction proceeds via initial assembly of the transposition complex that directs transposon ...
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