Cancer Biology


    Protocols in Current Issue
    A Fast and Reliable Method to Generate Pure, Single Cell-derived Clones of Mammalian Cells
    Authors:  Zhe Han, Bindhu K. Madhavan, Serap Kaymak, Peter Nawroth and Varun Kumar, date: 08/20/2022, view: 1115, Q&A: 0

    Stable cell cloning is an essential aspect of biological research. All advanced genome editing tools rely heavily on stable, pure, single cell-derived clones of genetically engineered cells. For years, researchers have depended on single-cell dilutions seeded in 96- or 192-well plates, followed by microscopic exclusion of the wells seeded with

    Soft Agar Colony Formation Assay as a Hallmark of Carcinogenesis
    Authors:  Feng Du, Xiaodi Zhao and Daiming Fan, date: 06/20/2017, view: 26957, Q&A: 0
    [Abstract] Soft agar colony formation assay is established to estimate the anchorage-independent growth ability of cells. In this assay, a bottom layer of agar with complete media is poured and solidified first, followed by an upper layer containing a specified number of cells suspended in medium-agar mixture. After two weeks of incubation, the number of ...
    Isolation of Murine Alveolar Type II Epithelial Cells
    Authors:  Fan Sun, Gutian Xiao and Zhaoxia Qu, date: 05/20/2017, view: 10290, Q&A: 0
    [Abstract] We have optimized a protocol for isolation of alveolar type II epithelial cells from mouse lung. Lung cell suspensions are prepared by intratracheal instillation of dispase and agarose followed by mechanical disaggregation of the lungs. Alveolar type II epithelial cells are purified from these lung cell suspensions through magnetic-based negative ...
    Murine Bronchoalveolar Lavage
    Authors:  Fan Sun, Gutian Xiao and Zhaoxia Qu, date: 05/20/2017, view: 23028, Q&A: 1
    [Abstract] A basic Bronchoalveolar lavage (BAL) procedure in mouse is described here. Cells and fluids obtained from BAL can be analyzed by Hema3-staining, immunostaining, Fluorescence-activated cell sorting (FACS), PCR, bicinchoninic acid protein assay, enzyme-linked immunosorbent assay (ELISA), luminex assays, etc., to examine the immune cells, ...
    Preparation of Primary Astrocyte Culture Derived from Human Glioblastoma Multiforme Specimen
    Authors:  Mansoureh Hashemi and Mahmoudreza Hadjighassem, date: 04/20/2017, view: 9644, Q&A: 0
    [Abstract] Glioblastoma multiforme (GBM) is a grade 4 astrocytoma tumor in central nervous system. Astrocytes can be isolated from human GBM. Study of astrocytes can provide insights about the formation, progression and recurrence of glioblastoma. For using isolated astrocytes, new studies can be designed in the fields of pharmacology, neuroscience and ...
    Generation of Mouse Thyroid Calcitonin-producing Cell Tumors from Primary Mouse Tumors
    Authors:  Shunsuke Kitajima, Fengkai Li and Chiaki Takahashi, date: 12/20/2015, view: 7660, Q&A: 0
    [Abstract] Medullary thyroid cancers (MTCs) are derived from calcitonin-producing cells (C cells) of neuroendocrine origin. Rb heterozygous mice develop low-grade C cell adenocarcinoma following biallelic inactivation of the Rb tumor suppressor gene loci. Additional inactivation of another tumor suppressor gene such as Trp53, Arf ...
    Analysis of Tumor-infiltrating Lymphocytes Following CD45 Enrichment
    [Abstract] Measuring antigen-specific T cell responses in the blood and lymphoid organs of vaccinated mice can give us a useful indication of the potency of a vaccine formulation. Unfortunately, systemic or even localized lymphoid T cell responses are not always predictive of the ability of a vaccine to induce tumor protection. Measuring the antigen-specific ...
    Transwell Co-culture of Bone Marrow Macrophages with Tumor Cells
    Authors:  Mackenzie K. Herroon and Izabela Podgorski, date: 05/05/2014, view: 20777, Q&A: 0
    [Abstract] Bone is a primary site of metastasis from prostate and breast cancers. Bone marrow macrophages (BMMs) are mediators of inflammatory processes and are thought to promote tumor growth in the skeletal sites. In order to elucidate how their interactions with tumor cells impact aggressiveness of metastatic tumors in bone in vitro methods are ...
    Pancreatic Acinar Cell 3-Dimensional Culture
    Authors:  Chunjing Qu and Stephen F. Konieczny, date: 10/05/2013, view: 13355, Q&A: 0
    [Abstract] Normal pancreatic acinar cells are difficult to maintain on traditional plastic culture surfaces due to their physical properties of housing large quantities of digestive enzymes and the formation of intercellular tight junctions and gap junctions (Apte and Wilson 2005; Rukstalis et al., 2003). However, placing primary acinar cells within ...
    Isolation of Mouse Embryo Fibroblasts
    Authors:  Marian E Durkin, Xiaolan Qian, Nicholas C Popescu and Douglas R Lowy, date: 09/20/2013, view: 17914, Q&A: 0
    [Abstract] Preparation of primary cultures of embryo fibroblasts from genetically engineered mouse strains can provide a valuable resource for analyzing the consequences of genetic alterations at the cellular level. Mouse embryo fibroblasts (MEFs) have been particularly useful in cancer research, as they have facilitated the identification of the genetic ...

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