Protocols in Current Issue
    Quantification of HIV-1 DNA
    Authors:  Ferdinand Roesch and Olivier Schwartz, date: 04/05/2013, view: 10811, Q&A: 0
    [Abstract] The reverse transcription (RT) reaction is a critical step in HIV-1 life cycle. It is very strongly regulated and the target of several restriction factors (TRIM5α, APOBECs, SAMHD1, etc.). The progress of reverse transcription can be followed by measuring viral DNA by quantitative PCR (qPCR). This method is sensitive enough to allow detection of ...

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