Stem Cell

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    Protocols in Current Issue
    Isolation of CD31+ Bone Marrow Endothelial Cells (BMECs) from Mice
    Authors:  Alhaji Osman Smith, Seyram Yao Adzraku, Wen Ju, Jianlin Qiao, Kailin Xu and Lingyu Zeng, date: 11/20/2021, view: 1371, Q&A: 0
    [Abstract]

    In the bone marrow microenvironment, endothelial cells (ECs) play a pivotal role in regulating the production of both growth and inhibiting factors. They are held together by adherence molecules that interact with hematopoietic progenitor cells. The study of ECs in the hematopoietic stem cell niche is limited due to the lack of efficient protocols

    ...
    In vitro Differentiation of Human iPSC-derived Cardiovascular Progenitor Cells (iPSC-CVPCs)
    Authors:  Agnieszka D’Antonio-Chronowska, Matteo D’Antonio and Kelly A. Frazer, date: 09/20/2020, view: 4861, Q&A: 1
    [Abstract] Induced pluripotent stem cell derived cardiovascular progenitor cells (iPSC-CVPCs) provide an unprecedented platform for examining the molecular underpinnings of cardiac development and disease etiology, but also have great potential to play pivotal roles in the future of regenerative medicine and pharmacogenomic studies. Biobanks like iPSCORE ( ...
    In vitro Co-culture of Mesenchymal Stem Cells and Endothelial Colony Forming Cells
    Authors:  Abbas Shafiee and Kiarash Khosrotehrani, date: 10/20/2017, view: 12453, Q&A: 0
    [Abstract] The discovery of endothelial colony forming cells (ECFCs) with robust self-renewal and de novo vessel formation potentials suggests that ECFCs can be an excellent cell source for cardiovascular diseases treatment through improving neovascularization in the ischemic tissues. However, their engraftment after transplantation resulted to be low. ...
    Mimicking Angiogenesis in vitro: Three-dimensional Co-culture of Vascular Endothelial Cells and Perivascular Cells in Collagen Type I Gels
    Authors:  Markus Auler, Lena Pitzler, Ernst Pöschl, Zhigang Zhou and Bent Brachvogel, date: 04/20/2017, view: 15729, Q&A: 0
    [Abstract] Angiogenesis defines the process of formation of new vascular structures form existing blood vessels, involved during development, repair processes like wound healing but also linked to pathological changes. During angiogenic processes, endothelial cells build a vascular network and recruit perivascular cells to form mature, stable vessels. ...
    Bone Marrow-derived Endothelial Progenitor Cell Intercellular Adhesion Assay
    Authors:  Yubo Tang, Angela Jacobi, Corina Vater, Xuenong Zou and Maik Stiehler, date: 08/20/2016, view: 9289, Q&A: 0
    [Abstract] Cell-cell adhesion ensures tight contacts between neighbouring cells, which is necessary for cell segregation, as well as for the morphological and functional differentiation of different tissues. Evidently there are cell-cell recognition systems that make cells of the same type preferentially adherent to one another. Homotypic cell adhesion is ...



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