Cell Biology


    Protocols in Current Issue
    In vivo Ca2+ Imaging in Mouse Salivary Glands
    Authors:  Takahiro Takano and David I. Yule, date: 04/05/2022, view: 682, Q&A: 0

    Changes in intracellular calcium drive exocrine cell activity. In the salivary gland, acetylcholine released from parasympathetic neurons mobilizes endoplasmic reticulum calcium stores in acinar cells, which consequently initiates saliva secretion. However, our understanding of the signaling cascade is mainly based on ex vivo studies

    Measurement of Contractile Ring Tension Using Two-photon Laser Ablation during Drosophila Cellularization
    Authors:  Swati Sharma and Richa Rikhy, date: 03/20/2022, view: 394, Q&A: 0

    Cytokinesis occurs at the final step of cell division and leads to the separation of daughter cells. It requires assembly and constriction of the actomyosin contractile ring. The phases of assembly and constriction of the contractile ring show an increase in tension in the actomyosin complex. The measurement of tension in the contractile ring is

    Characterization of Immunological Niches within Peyer’s Patches by ex vivo Photoactivation and Flow Cytometry Analysis
    Authors:  Adi Biram and Ziv Shulman, date: 03/20/2020, view: 3120, Q&A: 0
    [Abstract] T follicular helper (Tfh) cells regulate B cell selection for entry into the germinal center (GC) reaction or for differentiation into antibody forming cells. This process takes place at the border between the T and B zones in lymphoid organs and involves physical contacts between T and B cells. During these interactions, T cells endow the B cells ...
    Photothrombotic Induction of Capillary Ischemia in the Mouse Cortex during in vivo Two-Photon Imaging
    Authors:  Robert G. Underly and Andy Y. Shih, date: 07/05/2017, view: 8093, Q&A: 0
    [Abstract] Photothrombosis of blood vessels refers to the activation of a circulating photosensitive dye with a green light to induce clotting in vivo (Watson et al., 1985). Previous studies have described how a focused green laser could be used to noninvasively occlude pial arterioles and venules at the brain surface (Schaffer et al. ...
    Measurement of Mechanical Tension at cell-cell junctions using two-photon laser ablation
    Authors:  Xuan Liang, Magdalene Michael and Guillermo A. Gomez, date: 12/20/2016, view: 8873, Q&A: 1
    [Abstract] The cortical actomyosin cytoskeleton is found in all non-muscle cells where a key function is to control mechanical force (Salbreux et al., 2012). When coupled to E-cadherin cell-cell adhesion, cortical actomyosin generates junctional tension that influences many aspects of tissue function, organization and morphogenesis (Lecuit and Yap, ...
    Analysis of Developing Pollen Grains within Intact Arabidopsis thaliana Anthers by Olympus Two-Photon Laser Scanning Microscopy
    Authors:  Teagen D. Quilichini, A. Lacey Samuels and Carl J. Douglas, date: 12/05/2015, view: 8824, Q&A: 0
    [Abstract] The method consists of imaging developing pollen grains as they form within intact, immature Arabidopsis thaliana anthers. Using two-photon excitation in the infrared wavelength range, the intrinsic fluorescence (autofluorescence) of developing pollen grains and surrounding sporophytic tissues of the anther wall, including the tapetum, ...
    Two-photon Photoactivation to Measure Histone Exchange Dynamics in Plant Root Cells
    Authors:  Stefanie Rosa and Peter Shaw, date: 10/20/2015, view: 7199, Q&A: 0
    [Abstract] Chromatin-binding proteins play a crucial role in chromatin structure and gene expression. Direct binding of chromatin proteins both maintains and regulates transcriptional states. It is therefore important to study the binding properties of these proteins in vivo within the natural environment of the nucleus. Photobleaching, ...

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