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Protocol for Isolation of Cardiomyocyte from Adult Mouse and Rat

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Multi-color Flow Cytometry for Comprehensive Analysis of the Tumor Immune Infiltrate in a Murine Model of Breast Cancer

Authors: Ana S. Almeida, Miriam R. Fein and Mikala Egeblad, date: 06/05/2021, view: 2974, Q&A: 0

[Abstract]

Flow cytometry is a popular laser-based technology that allows the phenotypic and functional characterization of individual cells in a high-throughput manner. Here, we describe a detailed procedure for preparing a single-cell suspension from mammary tumors of the mouse mammary tumor virus-polyoma middle T (MMTV-PyMT) and analyzing these cells by

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In-vivo gp100-specific Cytotoxic CD8⁺ T Cell Killing Assay

Authors: Mahdia Benkhoucha, Nicolas Molnarfi, Elodie Belnoue, Madiha Derouazi and Patrice H Lalive, date: 11/20/2018, view: 6202, Q&A: 0

[Abstract] Cytotoxic CD8⁺ T lymphocytes (CTLs) represent a crucial component of the adaptive immune system and play a prominent role in the anti-tumor immune responses of both mice and humans.

Cytotoxic CD8⁺ T cells are responsible for the lysis of cells expressing peptides associated with MHC class I molecules and derived ...

Glioma Induction by Intracerebral Retrovirus Injection

Authors: Ravinder K Verma , Fanghui Lu and Qing Richard Lu, date: 07/20/2017, view: 8292, Q&A: 0

[Abstract] Glioblastoma (GBM) is the most common primary brain cancer in adults and has a poor prognosis. It is characterized by a high degree of cellular infiltration that leads to tumor recurrence, atypical hyperplasia, necrosis, and angiogenesis. Despite aggressive treatment modalities, current therapies are ineffective for GBM. Mouse GBM models not only ...

Determining Leukocyte Origins Using Parabiosis in the PyMT Breast Tumor Model

Authors: Ruth A. Franklin and Ming O. Li, date: 08/20/2015, view: 8931, Q&A: 0

[Abstract] Tumors develop in a complex microenvironment alongside numerous cell types that impact their survival. Immune cells make up a large proportion of these accessory cells and many are known to promote tumor progression. Macrophages, in particular, are associated with poor patient prognosis and are therefore potential candidates for therapeutic ...

Adoptive Transfer of Myeloid-Derived Suppressor Cells and T Cells in a Prostate Cancer Model

Authors: Libo Yan and Yan Xu, date: 08/20/2015, view: 8101, Q&A: 0

[Abstract] The adoptive transfer of immune cells for cancer, chronic infection, and autoimmunity is an emerging field that has shown promise in recent trials. The transgenic adenocarcinoma mouse prostate (TRAMP) is a classical mouse model of prostate cancer (PCa) and TRAMP cell lines were derived from a TRAMP mouse tumor. TRAMP-C2 is tumorigenic when ...

Purification of Tumor-Associated Macrophages (TAM) and Tumor-Associated Dendritic Cells (TADC)

Authors: Damya Laoui, Eva Van Overmeire, Jiri Keirsse, Kiavash Movahedi and Jo A Van Ginderachter, date: 11/20/2014, view: 25544, Q&A: 1

[Abstract] Tumors are heterogeneous microenvironments where complex interactions take place between neoplastic cells and infiltrating inflammatory cells, such as tumor-associated macrophages (TAM) and tumor-associated dendritic cells (TADC). The relevance of tumor-infiltrating mononuclear myeloid cells is underscored by clinical studies showing a correlation ...

Natural Killer Cell Transfer Assay

Authors: Lee-Hwa Tai, Christiano Tanese de Souza, Andrew P. Makrigiannis and Rebecca Ann C. Auer, date: 08/20/2013, view: 11115, Q&A: 0

[Abstract] Natural Killer (NK) cells are cytotoxic lymphocytes that constitute a major component of the innate immune system. Immunosurveillance of the host by NK cells for malignant and virally-infected cells results in direct cytotoxicity and the production of cytokines to enhance the immune response. This protocol will describe the adoptive transfer of ...

Lung Clearance Assay

Authors: Pinchas Tsukerman and Ofer Mandelboim, date: 03/05/2013, view: 9605, Q&A: 0

[Abstract] Lung clearance assay tests the ability of innate immune cells (mainly NK cells) to eradicate in vivo cells injected via the tail vein of the mice. This assay helps to elucidate the role played by NK cells and their receptors (if the mice are genetically modified) against various human and mouse targets in an in vivo setting ...

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