Cancer Biology


    Protocols in Current Issue
    Analysis of Leukemia Cell Metabolism through Stable Isotope Tracing in Mice
    Authors:  Nick van Gastel, Jessica B. Spinelli, Marcia C. Haigis and David T. Scadden, date: 10/05/2021, view: 1930, Q&A: 0

    Once thought to be a mere consequence of the state of a cell, intermediary metabolism is now recognized as a key regulator of mammalian cell fate and function. In addition, cell metabolism is often disturbed in malignancies such as cancer, and targeting metabolic pathways can provide new therapeutic options. Cell metabolism is mostly studied in

    Stable Isotope Resolved Metabolomics Studies in ex vivo TIssue Slices
    Authors:  Teresa W-M. Fan, Andrew N. Lane and Richard M. Higashi, date: 02/05/2016, view: 9352, Q&A: 0
    [Abstract] An important component of this methodology is to assess the role of the tumor microenvironment on tumor growth and survival. To tackle this problem, we have adapted the original approach of Warburg (Warburg, 1923), by combining thin tissue slices with Stable Isotope Resolved Metabolomics (SIRM) to determine detailed metabolic activity of human ...
    13C Tracer Studies of Metabolism in Mouse Tumor Xenografts
    Authors:  Andrew N. Lane, Jun Yan and Teresa W-M. Fan, date: 11/20/2015, view: 10457, Q&A: 0
    [Abstract] Mice are widely used for human tumor xenograft studies of cancer development and drug efficacy and toxicity. Stable isotope tracing coupled with metabolomic analysis is an emerging approach for assaying metabolic network activity. In mouse models there are several routes of tracer introduction, which have particular advantages and disadvantages ...
    Hypoxia Studies with Pimonidazole in vivo
    Authors:  Kristina Y. Aguilera and Rolf A. Brekken, date: 10/05/2014, view: 24105, Q&A: 0
    [Abstract] Therapy-induced hypoxia drives changes in the tumor microenvironment that contribute to the poor response to therapy. Hypoxia is capable of driving the expression and/or activation of specific signaling cascades (e.g., c-Met, Axl, CTGF), the recruitment of tumor promoting immune cells, and the induction of cell survival pathways including ...

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