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Rapid Profiling Cell Cycle by Flow Cytometry Using Concurrent Staining of DNA and Mitotic Markers
Authors:
Yuqing Shen
,
Paolo Vignali
and
Ruoning Wang
,
date:
08/20/2017,
view:
10389,
Q&A:
1
[Abstract] The flow cytometric quantitation of DNA content by DNA-binding fluorochrome, propidium iodide (PI) is the most widely used method for cell cycle analysis. However, the commonly used methods are time-consuming and labor-intensive and are incompatible with staining of mitotic markers by fluorescent-labeled antibodies. Here, we report an optimized ...
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