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Protocol for Isolation of Cardiomyocyte from Adult Mouse and Rat
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Direct Reprogramming of Mouse Embryonic Fibroblasts to Conventional Type 1 Dendritic Cells by Enforced Expression of Transcription Factors
Authors:
Fábio F. Rosa
,
Cristiana F. Pires
,
Olga Zimmermannova
and
Carlos-Filipe Pereira
,
date:
05/20/2020,
view:
4157,
Q&A:
0
[Abstract] Ectopic expression of transcription factor combinations has been recently demonstrated to reprogram differentiated somatic cells towards the dendritic cell (DC) lineage without reversion to a multipotent state. DCs have the ability to induce potent and long-lasting adaptive immune responses. In particular, conventional type 1 DCs (cDC1s) excel on ...
Isolation of Splenic Dendritic Cells Using Fluorescence-activated Cell Sorting
Authors:
Simon J Tavernier
,
Fabiola Osorio
,
Sophie Janssens
and
Bart N Lambrecht
,
date:
03/05/2015,
view:
15315,
Q&A:
0
[Abstract] The spleen is a vastly vasculated organ and consists of a complex organized network of innate and adaptive immune cells. This permits the specialized functions of the spleen such as antibacterial and antifungal immunity and iron metabolism among others (Mebius and Kraal, 2005). Different dendritic cell (DC) subsets reside in the spleen and can be ...
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