Protocols in Current Issue
    Aerotaxis Assay in Caenorhabditis elegans to Study Behavioral Plasticity
    Authors:  Qiaochu Li, Daniel-Cosmin Marcu, Paul H. Dear and Karl Emanuel Busch, date: 08/20/2022, view: 966, Q&A: 0

    C. elegans shows robust and reproducible behavioral responses to oxygen. Specifically, worms prefer O2 levels of 5–10% and avoid too high or too low O2. Their O2 preference is not fixed but shows plasticity depending on experience, context, or genetic background. We recently showed that this experience-dependent plasticity declines with age,

    Time-off-pick Assay to Measure Caenorhabditis elegans Motility
    Authors:  Alyssa C. Walker, Rohan Bhargava, Amanda S. Brust, Ali A. Owji and Daniel M. Czyż, date: 06/20/2022, view: 1592, Q&A: 0

    Caenorhabditis elegans is a simple metazoan that is often used as a model organism to study various human ailments with impaired motility phenotypes, including protein conformational diseases. Numerous motility assays that measure neuro-muscular function have been employed using C. elegans. Here, we describe “time-off-pick" (TOP), a novel

    Measuring Spatiotemporal Dynamics of Odor Gradient for Small Animals by Gas Chromatography
    Authors:  Akiko Yamazoe-Umemoto, Yuishi Iwasaki and Koutarou D. Kimura, date: 04/05/2018, view: 6305, Q&A: 0
    [Abstract] Odor is the most fundamental chemical stimulus that delivers information regarding food, mating partners, enemies, and danger in the surrounding environment. Research on odor response in animals is widespread, although studies on experimental systems in which the gradient of odor concentration is quantitatively measured has been quite limited. ...
    Plate Assay to Determine Caenorhabditis elegans Response to Water Soluble and Volatile Chemicals
    Authors:  Takashi Murayama and Ichiro N. Maruyama, date: 02/20/2018, view: 7942, Q&A: 0
    [Abstract] The nematode Caenorhabditis elegans is widely used for behavioral studies ranging from simple chemosensation to associative learning and memory. It is vital for such studies to determine optimal concentrations of attractive and aversive chemicals that C. elegans can sense. Here we describe a resource localization assay in which a ...
    Chemotaxis and Jumping Assays in Nematodes
    Authors:  Tiffany Baiocchi and Adler R. Dillman, date: 09/20/2015, view: 10450, Q&A: 0
    [Abstract] Nematodes have sensitive olfactory perception, which is used to detect and differentiate many volatile odorants. Some odorants are attractive, others repulsive, and yet others evoke no particular response. Chemotaxis assays can be used to determine the role of certain odors in many different behaviors including foraging, predator avoidance, and ...
    In situ Chemotaxis Assay in Caenorhabditis elegans (for the Study of Circadian Rhythms)
    Authors:  Martha Merrow and Maria Olmedo, date: 02/05/2014, view: 13179, Q&A: 0
    [Abstract] Olfaction is a well-studied sensory mechanism in Caenorhabditis elegans (C. elegans). The nematodes respond to a wide range of chemicals by either attraction, repulsion or a mixture thereof (Bargmann et al., 1993). We have used olfaction to characterize behavioural and molecular circadian rhythms in C. elegans. ...

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